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. 1984 May;47(5):901–904. doi: 10.1128/aem.47.5.901-904.1984

Growth of oral Streptococcus species and Actinomyces viscosus in human saliva.

M H de Jong, J S van der Hoeven, J H van OS, J H Olijve
PMCID: PMC240009  PMID: 6742834

Abstract

Microorganisms in dental plaque live in constant association with saliva. The role of saliva in the adherence of bacteria to the teeth and the antibacterial properties of saliva have been well investigated; less interest has been shown in the possible role of saliva as a substrate for oral microorganisms. In this study it was shown that saliva can serve as a growth medium for oral Streptococcus spp. and Actinomyces viscosus. The cell production of these organisms on saliva was carbohydrate limited. The doubling times for growth on glucose-supplemented saliva (4 to 5 mmol/liter) ranged from 1.6 to 4.0 h. The availability of carbohydrate sources for the oral microflora is discussed in relation to microbial growth in the oral cavity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beckers H. J., van der Hoeven J. S. Growth rates of Actinomyces viscosus and Streptococcus mutans during early colonization of tooth surfaces in gnotobiotic rats. Infect Immun. 1982 Feb;35(2):583–587. doi: 10.1128/iai.35.2.583-587.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brecher S. M., van Houte J. Relationship between host age and susceptibility to oral colonization by Actinomyces viscosus in Sprague-Dawley rats. Infect Immun. 1979 Dec;26(3):1137–1145. doi: 10.1128/iai.26.3.1137-1145.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carlsson J., Griffith C. J. Fermentation products and bacterial yields in glucose-limited and nitrogen-limited cultures of streptococci. Arch Oral Biol. 1974 Dec;19(12):1105–1109. doi: 10.1016/0003-9969(74)90238-6. [DOI] [PubMed] [Google Scholar]
  4. Cowman R. A., Fitzgerald R. J., Perrella M. M., Cornell A. H. Human saliva as a nitrogen source for oral streptococci. Caries Res. 1977;11(1):1–8. doi: 10.1159/000260242. [DOI] [PubMed] [Google Scholar]
  5. Cowman R. A., Perrella M. M., Adams B. O., Fitzgerald R. J. Amino acid requirements and proteolytic activity of Streptococcus sanguis. Appl Microbiol. 1975 Sep;30(3):374–380. doi: 10.1128/am.30.3.374-380.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cowman R. A., Perrella M. M., Fitzgerald R. J. Caseinolytic and glyoprotein hydrolase activity of Streptococcus mutans. J Dent Res. 1976 May-Jun;55(3):391–399. doi: 10.1177/00220345760550031701. [DOI] [PubMed] [Google Scholar]
  7. Cowman R. A., Schaefer S. J., Fitzgerald R. J. Specificity of utilization of human salivary proteins for growth by oral streptococci. Caries Res. 1979;13(4):181–189. doi: 10.1159/000260399. [DOI] [PubMed] [Google Scholar]
  8. Dawes C., Dowse C. M., Knull H. R. Stop-flow effects on human salivary composition and hydrostatic pressures. Arch Oral Biol. 1980;25(4):251–256. doi: 10.1016/0003-9969(80)90030-8. [DOI] [PubMed] [Google Scholar]
  9. Donkersloot J. A., Robrish S. A., Krichevsky M. I. Fluorometric determination of deoxyribonucleic acid in bacteria with ethidium bromide. Appl Microbiol. 1972 Aug;24(2):179–183. doi: 10.1128/am.24.2.179-183.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gibbons R. J., Houte J. V. Bacterial adherence in oral microbial ecology. Annu Rev Microbiol. 1975;29:19–44. doi: 10.1146/annurev.mi.29.100175.000315. [DOI] [PubMed] [Google Scholar]
  11. Jones M. G., Outlaw W. H., Lowry O. H. Enzymic assay of 10 to 10 moles of sucrose in plant tissues. Plant Physiol. 1977 Sep;60(3):379–383. doi: 10.1104/pp.60.3.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kelsay J. L., McCague K. E., Holden J. M. Variations in flow rate, metabolites and enzyme activities of fasting human parotid saliva. Arch Oral Biol. 1972 Mar;17(3):439–445. doi: 10.1016/0003-9969(72)90059-3. [DOI] [PubMed] [Google Scholar]
  13. Leach S. A., Critchley P. Bacterial degradation of glycoprotein sugars in human saliva. Nature. 1966 Jan 29;209(5022):506–506. doi: 10.1038/209506a0. [DOI] [PubMed] [Google Scholar]
  14. Leach S. A., Melville T. H. Investigation of some human oral organisms capable of releasing the carbohydrates from salivary glycoproteins. Arch Oral Biol. 1970 Jan;15(1):87–88. doi: 10.1016/0003-9969(70)90147-0. [DOI] [PubMed] [Google Scholar]
  15. Leah S. A., Hayes M. L. Isolation in pure culture of human oral organisms capable of producing neuraminidase. Nature. 1967 Nov 11;216(5115):599–600. doi: 10.1038/216599a0. [DOI] [PubMed] [Google Scholar]
  16. SHIOTA T., KUNKEL M. F., Jr In vitro chemical and bacterial changes in saliva. J Dent Res. 1958 Sep-Oct;37(5):780–787. doi: 10.1177/00220345580370050301. [DOI] [PubMed] [Google Scholar]
  17. Socransky S. S., Manganiello A. D., Propas D., Oram V., van Houte J. Bacteriological studies of developing supragingival dental plaque. J Periodontal Res. 1977 Mar;12(2):90–106. doi: 10.1111/j.1600-0765.1977.tb00112.x. [DOI] [PubMed] [Google Scholar]
  18. WILLIAMS N. B., POWLEN D. O. Human parotid saliva as a sole source of nutrient for micro-organisms. Arch Oral Biol. 1959 Aug;1:48–61. doi: 10.1016/0003-9969(59)90021-4. [DOI] [PubMed] [Google Scholar]
  19. van der Hoeven J. S., Franken H. C., Camp P. J., Dellebarre C. W. Analysis of bacterial fermentation products by isotachophoresis. Appl Environ Microbiol. 1978 Jan;35(1):17–23. doi: 10.1128/aem.35.1.17-23.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. van der Hoeven J. S., Franken H. C. Production of acids in rat dental plaque with or without Streptococcus mutans. Caries Res. 1982;16(5):375–383. doi: 10.1159/000260623. [DOI] [PubMed] [Google Scholar]

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