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. 1984 May;47(5):1012–1016. doi: 10.1128/aem.47.5.1012-1016.1984

Protein synthesis in Escherichia coli during recovery from exposure to low levels of Cd2+.

R S Mitra
PMCID: PMC240042  PMID: 6204593

Abstract

Exposure of Escherichia coli to 3 microM Cd2+ results in 84 to 95% of the cells losing their ability to form colonies on plates of nutrient agar. Transfer of the cells to Cd2+-free liquid medium results in a recovery of colony-forming ability without significant synthesis of DNA. As an early event in recovery, the cells exhibit a rapid uptake of [3H]leucine. Recovery and this incorporation are inhibited by chloramphenicol or rifampin. Sodium dodecyl sulfate-gel electrophoresis of proteins from recovering cells labeled with [3H]leucine for 1 min indicated the synthesis of at least two classes of proteins with apparent molecular weights of 55,000 to 65,000. One class bound Cd2+ and was absent in untreated cultures. The other class of proteins, which did not bind Cd2+, was synthesized at a rapid rate in recovering cells and may be a normal cellular protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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