Abstract
Ruminococcus albus 8 was cultured with isolated alfalfa cell walls as the carbon source. The culture broth was assayed for muralytic enzyme activities. The effect, with respect to the production of such muralytic enzymes, of growing the microorganism on different carbon sources was also investigated. Also, the rates of solubilization and utilization by R. albus of individual alfalfa cell wall sugars during a 96-h growth period were examined.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Blumenkrantz N., Asboe-Hansen G. New method for quantitative determination of uronic acids. Anal Biochem. 1973 Aug;54(2):484–489. doi: 10.1016/0003-2697(73)90377-1. [DOI] [PubMed] [Google Scholar]
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
- Clarke R. T., Bailey R. W., Gaillard B. D. Growth of rumen bacteria on plant cell wall polysaccharides. J Gen Microbiol. 1969 Apr;56(1):9–86. [PubMed] [Google Scholar]
- Dehority B. A. Hemicellulose degradation by rumen bacteria. Fed Proc. 1973 Jul;32(7):1819–1825. [PubMed] [Google Scholar]
- Greve L. C., Labavitch J. M., Hungate R. E. alpha-L-arabinofuranosidase from Ruminococcus albus 8: purification and possible role in hydrolysis of alfalfa cell wall. Appl Environ Microbiol. 1984 May;47(5):1135–1140. doi: 10.1128/aem.47.5.1135-1140.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HUNGATE R. E. Microorganisms in the rumen of cattle fed a constant ration. Can J Microbiol. 1957 Mar;3(2):289–311. doi: 10.1139/m57-034. [DOI] [PubMed] [Google Scholar]
- Hungate R. E., Stack R. J. Phenylpropanoic Acid: Growth Factor for Ruminococcus albus. Appl Environ Microbiol. 1982 Jul;44(1):79–83. doi: 10.1128/aem.44.1.79-83.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Labavitch J. M., Freeman L. E., Albersheim P. Structure of plant cell walls. Purification and characterization of a beta-1,4-galactanase which degrades a structural component of the primary cell walls of dicots. J Biol Chem. 1976 Oct 10;251(19):5904–5910. [PubMed] [Google Scholar]
- SMOGYI M. Notes on sugar determination. J Biol Chem. 1952 Mar;195(1):19–23. [PubMed] [Google Scholar]
- Smith W. R., Yu I., Hungate R. E. Factors affecting cellulolysis by Ruminococcus albus. J Bacteriol. 1973 May;114(2):729–737. doi: 10.1128/jb.114.2.729-737.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stack R. J., Hungate R. E., Opsahl W. P. Phenylacetic acid stimulation of cellulose digestion by Ruminococcus albus 8. Appl Environ Microbiol. 1983 Sep;46(3):539–544. doi: 10.1128/aem.46.3.539-544.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taiz L., Honigman W. A. Production of cell wall hydrolyzing enzymes by barley aleurone layers in response to gibberellic Acid. Plant Physiol. 1976 Sep;58(3):380–386. doi: 10.1104/pp.58.3.380. [DOI] [PMC free article] [PubMed] [Google Scholar]