Abstract
We report the use of charge-shift electrophoresis to define the behavior of the trichothecene mycotoxin T-2 in aqueous solutions. We found that T-2 behaves in a hydrophobic manner and that this technique can be adapted for the separation of small hydrophobic molecules such as T-2 and cholesterol.
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Selected References
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- Armstrong D. W., Fendler J. H. Differential partitioning of tRNAs between micellar and aqueous phases: a convenient gel filtration method for separation of tRNAs. Biochim Biophys Acta. 1977 Sep 6;478(1):75–80. doi: 10.1016/0005-2787(77)90245-3. [DOI] [PubMed] [Google Scholar]
- Bjerrum O. J., Bhakdi S., Rieneck K. Monitoring of detergent binding to amphiphilic proteins by means of micelles containing the hydrophobic dye Sudan Black B. J Biochem Biophys Methods. 1980 Dec;3(6):355–366. doi: 10.1016/0165-022x(80)90044-5. [DOI] [PubMed] [Google Scholar]
- Dalgliesh C. E., Horning E. C., Horning M. G., Knox K. L., Yarger K. A gas-liquid-chromatographic procedure for separating a wide range of metabolites occuring in urine or tissue extracts. Biochem J. 1966 Dec;101(3):792–810. doi: 10.1042/bj1010792. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Helenius A., Simons K. Charge shift electrophoresis: simple method for distinguishing between amphiphilic and hydrophilic proteins in detergent solution. Proc Natl Acad Sci U S A. 1977 Feb;74(2):529–532. doi: 10.1073/pnas.74.2.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Helenius A., Simons K. Solubilization of membranes by detergents. Biochim Biophys Acta. 1975 Mar 25;415(1):29–79. doi: 10.1016/0304-4157(75)90016-7. [DOI] [PubMed] [Google Scholar]
- Schappert K. T., Khachatourians G. G. Effects of Fusariotoxin T-2 on Saccharomyces cerevisiae and Saccharomyces carlsbergensis. Appl Environ Microbiol. 1983 Mar;45(3):862–867. doi: 10.1128/aem.45.3.862-867.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schappert K. T., Khachatourians G. G. Influence of the membrane on T-2 toxin toxicity in Saccharomyces spp. Appl Environ Microbiol. 1984 Apr;47(4):681–684. doi: 10.1128/aem.47.4.681-684.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ueno Y., Nakajima M., Sakai K., Ishii K., Sato N. Comparative toxicology of trichothec mycotoxins: inhibition of protein synthesis in animal cells. J Biochem. 1973 Aug;74(2):285–296. [PubMed] [Google Scholar]
