Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1992 Nov;66(11):6813–6818. doi: 10.1128/jvi.66.11.6813-6818.1992

Transcription of human respiratory syncytial virus genome RNA in vitro: requirement of cellular factor(s).

S Barik 1
PMCID: PMC240184  PMID: 1404620

Abstract

Extracts made from human respiratory syncytial virus (RSV)-infected Hep-2 cells synthesized mRNAs encoded by all known viral genes. In contrast, RSV ribonucleoproteins purified from infected cells failed to transcribe in vitro; transcription was restored by addition of a cytoplasmic extract of uninfected Hep-2 cells, demonstrating that a cellular factor(s) has a role in RSV gene expression. Quantitation of the individual gene mRNAs transcribed in vitro revealed polarity of transcription of the genome.

Full text

PDF
6813

Images in this article

6814Image
on p.6814
6815Image
on p.6815
6815Image
on p.6815
6816Image
on p.6816

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banerjee A. K., Barik S., De B. P. Gene expression of nonsegmented negative strand RNA viruses. Pharmacol Ther. 1991;51(1):47–70. doi: 10.1016/0163-7258(91)90041-j. [DOI] [PubMed] [Google Scholar]
  2. Banerjee A. K., Barik S. Gene expression of vesicular stomatitis virus genome RNA. Virology. 1992 Jun;188(2):417–428. doi: 10.1016/0042-6822(92)90495-b. [DOI] [PubMed] [Google Scholar]
  3. Barik S., Banerjee A. K. Cloning and expression of the vesicular stomatitis virus phosphoprotein gene in Escherichia coli: analysis of phosphorylation status versus transcriptional activity. J Virol. 1991 Apr;65(4):1719–1726. doi: 10.1128/jvi.65.4.1719-1726.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berger S. L. Quantifying 32P-labeled and unlabeled nucleic acids. Methods Enzymol. 1987;152:49–54. doi: 10.1016/0076-6879(87)52009-2. [DOI] [PubMed] [Google Scholar]
  5. Carlsen S. R., Peluso R. W., Moyer S. A. In vitro replication of Sendai virus wild-type and defective interfering particle genome RNAs. J Virol. 1985 May;54(2):493–500. doi: 10.1128/jvi.54.2.493-500.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Collins P. L., Dickens L. E., Buckler-White A., Olmsted R. A., Spriggs M. K., Camargo E., Coelingh K. V. Nucleotide sequences for the gene junctions of human respiratory syncytial virus reveal distinctive features of intergenic structure and gene order. Proc Natl Acad Sci U S A. 1986 Jul;83(13):4594–4598. doi: 10.1073/pnas.83.13.4594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Collins P. L., Hightower L. E., Ball L. A. Transcription and translation of Newcastle disease virus mRNA's in vitro. J Virol. 1978 Oct;28(1):324–336. doi: 10.1128/jvi.28.1.324-336.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Collins P. L., Huang Y. T., Wertz G. W. Identification of a tenth mRNA of respiratory syncytial virus and assignment of polypeptides to the 10 viral genes. J Virol. 1984 Feb;49(2):572–578. doi: 10.1128/jvi.49.2.572-578.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Collins P. L., Wertz G. W. Nucleotide sequences of the 1B and 1C nonstructural protein mRNAs of human respiratory syncytial virus. Virology. 1985 Jun;143(2):442–451. doi: 10.1016/0042-6822(85)90384-8. [DOI] [PubMed] [Google Scholar]
  10. Collins P. L., Wertz G. W. The 1A protein gene of human respiratory syncytial virus: nucleotide sequence of the mRNA and a related polycistronic transcript. Virology. 1985 Mar;141(2):283–291. doi: 10.1016/0042-6822(85)90259-4. [DOI] [PubMed] [Google Scholar]
  11. Collins P. L., Wertz G. W. The envelope-associated 22K protein of human respiratory syncytial virus: nucleotide sequence of the mRNA and a related polytranscript. J Virol. 1985 Apr;54(1):65–71. doi: 10.1128/jvi.54.1.65-71.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Collins P. L., Wertz G. W. cDNA cloning and transcriptional mapping of nine polyadenylylated RNAs encoded by the genome of human respiratory syncytial virus. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3208–3212. doi: 10.1073/pnas.80.11.3208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. De B. P., Lesoon A., Banerjee A. K. Human parainfluenza virus type 3 transcription in vitro: role of cellular actin in mRNA synthesis. J Virol. 1991 Jun;65(6):3268–3275. doi: 10.1128/jvi.65.6.3268-3275.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Dickens L. E., Collins P. L., Wertz G. W. Transcriptional mapping of human respiratory syncytial virus. J Virol. 1984 Nov;52(2):364–369. doi: 10.1128/jvi.52.2.364-369.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Elango N., Satake M., Venkatesan S. mRNA sequence of three respiratory syncytial virus genes encoding two nonstructural proteins and a 22K structural protein. J Virol. 1985 Jul;55(1):101–110. doi: 10.1128/jvi.55.1.101-110.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Fernie B. F., Gerin J. L. Immunochemical identification of viral and nonviral proteins of the respiratory syncytial virus virion. Infect Immun. 1982 Jul;37(1):243–249. doi: 10.1128/iai.37.1.243-249.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Garcia-Barreno B., Jorcano J. L., Aukenbauer T., López-Galíndez C., Melero J. A. Participation of cytoskeletal intermediate filaments in the infectious cycle of human respiratory syncytial virus (RSV). Virus Res. 1988 Mar;9(4):307–321. doi: 10.1016/0168-1702(88)90090-1. [DOI] [PubMed] [Google Scholar]
  18. Hamaguchi M., Nishikawa K., Toyoda T., Yoshida T., Hanaichi T., Nagai Y. Transcriptive complex of Newcastle disease virus. II. Structural and functional assembly associated with the cytoskeletal framework. Virology. 1985 Dec;147(2):295–308. doi: 10.1016/0042-6822(85)90132-1. [DOI] [PubMed] [Google Scholar]
  19. Herman R. C. Synthesis of respiratory syncytial virus RNA in cell-free extracts. J Gen Virol. 1989 Mar;70(Pt 3):755–761. doi: 10.1099/0022-1317-70-3-755. [DOI] [PubMed] [Google Scholar]
  20. Huang Y. T., Collins P. L., Wertz G. W. Characterization of the 10 proteins of human respiratory syncytial virus: identification of a fourth envelope-associated protein. Virus Res. 1985 Mar;2(2):157–173. doi: 10.1016/0168-1702(85)90246-1. [DOI] [PubMed] [Google Scholar]
  21. Huang Y. T., Wertz G. W. The genome of respiratory syncytial virus is a negative-stranded RNA that codes for at least seven mRNA species. J Virol. 1982 Jul;43(1):150–157. doi: 10.1128/jvi.43.1.150-157.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Johnson P. R., Collins P. L. The 1B (NS2), 1C (NS1) and N proteins of human respiratory syncytial virus (RSV) of antigenic subgroups A and B: sequence conservation and divergence within RSV genomic RNA. J Gen Virol. 1989 Jun;70(Pt 6):1539–1547. doi: 10.1099/0022-1317-70-6-1539. [DOI] [PubMed] [Google Scholar]
  23. Johnson P. R., Spriggs M. K., Olmsted R. A., Collins P. L. The G glycoprotein of human respiratory syncytial viruses of subgroups A and B: extensive sequence divergence between antigenically related proteins. Proc Natl Acad Sci U S A. 1987 Aug;84(16):5625–5629. doi: 10.1073/pnas.84.16.5625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Lehrach H., Diamond D., Wozney J. M., Boedtker H. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry. 1977 Oct 18;16(21):4743–4751. doi: 10.1021/bi00640a033. [DOI] [PubMed] [Google Scholar]
  25. López J. A., Villanueva N., Melero J. A., Portela A. Nucleotide sequence of the fusion and phosphoprotein genes of human respiratory syncytial (RS) virus Long strain: evidence of subtype genetic heterogeneity. Virus Res. 1988 May;10(2-3):249–261. doi: 10.1016/0168-1702(88)90020-2. [DOI] [PubMed] [Google Scholar]
  26. Moyer S. A., Baker S. C., Horikami S. M. Host cell proteins required for measles virus reproduction. J Gen Virol. 1990 Apr;71(Pt 4):775–783. doi: 10.1099/0022-1317-71-4-775. [DOI] [PubMed] [Google Scholar]
  27. Moyer S. A., Baker S. C., Lessard J. L. Tubulin: a factor necessary for the synthesis of both Sendai virus and vesicular stomatitis virus RNAs. Proc Natl Acad Sci U S A. 1986 Aug;83(15):5405–5409. doi: 10.1073/pnas.83.15.5405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Peeples M., Levine S. Respiratory syncytial virus polypeptides: their location in the virion. Virology. 1979 May;95(1):137–145. doi: 10.1016/0042-6822(79)90408-2. [DOI] [PubMed] [Google Scholar]
  29. Pringle C. R., Shirodaria P. V., Gimenez H. B., Levine S. Antigen and polypeptide synthesis by temperature-sensitive mutants of respiratory syncytial virus. J Gen Virol. 1981 May;54(Pt 1):173–183. doi: 10.1099/0022-1317-54-1-173. [DOI] [PubMed] [Google Scholar]
  30. Ray J., Fujinami R. S. Characterization of in vitro transcription and transcriptional products of measles virus. J Virol. 1987 Nov;61(11):3381–3387. doi: 10.1128/jvi.61.11.3381-3387.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Stec D. S., Hill M. G., 3rd, Collins P. L. Sequence analysis of the polymerase L gene of human respiratory syncytial virus and predicted phylogeny of nonsegmented negative-strand viruses. Virology. 1991 Jul;183(1):273–287. doi: 10.1016/0042-6822(91)90140-7. [DOI] [PubMed] [Google Scholar]
  32. Wertz G. W., Davis N. L. RNase III cleaves vesicular stomatitis virus genome-length RNAs but fails to cleave viral mRNA's. J Virol. 1979 Apr;30(1):108–115. doi: 10.1128/jvi.30.1.108-115.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Wunner W. H., Pringle C. R. Respiratory syncytial virus proteins. Virology. 1976 Aug;73(1):228–243. doi: 10.1016/0042-6822(76)90077-5. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES