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. 1984 Jun;47(6):1348–1351. doi: 10.1128/aem.47.6.1348-1351.1984

Detection of streptococcal mutants presumed to be defective in sugar catabolism.

T W Feary, J A Mayo
PMCID: PMC240244  PMID: 6378096

Abstract

The tetrazolium method for detection of bacterial mutants defective in sugar catabolism was modified for use with streptococci. The critical factors were (i) the concentration of tetrazolium, which must be titrated to determine the optimum concentration for each species or even strain, and (ii) anaerobic incubation of tetrazolium-containing agar plates. When used with standard mutagenesis protocols, this method yielded lactose-negative mutants of nine streptococcal strains representing six species. A collection of lactose-negative mutants of streptococcus, sanguis Challis was characterized and contained phospho-beta-galactosidase, lactose phosphotransferase, and general phosphotransferase mutants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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