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. 1993 Apr;67(4):1976–1986. doi: 10.1128/jvi.67.4.1976-1986.1993

Characterization of a protein that binds multiple sequences in mammalian type C retrovirus enhancers.

W Sun 1, M O'Connell 1, N A Speck 1
PMCID: PMC240266  PMID: 8445719

Abstract

Mammalian type C retrovirus enhancer factor 1 (MCREF-1) is a nuclear protein that binds several directly repeated sequences (CNGGN6CNGG) in the Moloney and Friend murine leukemia virus (MLV) enhancers (N. R. Manley, M. O'Connell, W. Sun, N. A. Speck, and N. Hopkins, J. Virol. 67:1967-1975, 1993). In this paper, we describe the partial purification of MCREF-1 from calf thymus nuclei and further characterize the binding properties of MCREF-1. MCREF-1 binds four sites in the Moloney MLV enhancer and three sites in the Friend MLV enhancer. Ethylation interference analysis suggests that the MCREF-1 binding site spans two adjacent minor grooves of DNA.

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