Abstract
The successful nodulation of legumes by a Rhizobium strain is determined by the competitive ability of that strain against the mixture of other native and inoculant rhizobia. Competition among six Leucaena rhizobial strains in single and multistrain inoculants were studied. Field inoculation trials were conducted in an oxisol and a mollisol soil, both of which contained indigenous Leucaena-nodulating rhizobia. Strain-specific fluorescent antibodies were used for the identification of the strains in Leucaena nodules. Mixtures of three recommended inoculum strains for Leucaena spp. (TAL82, TAL582, and TAL1145) were used in peat-based inocula either alone or with one of the three other strains isolated from the sites, B213, B214, and B215. Each of these latter three strains was also used as single-strain inocula to study their competition with the native rhizobia in the two soil systems. In the oxisol soil, strains B213 and B215, when used as single-strain inocula, outcompeted the native rhizobia and formed 92 and 62% of the nodules, respectively. Strain B214 was the least competitive in oxisol soil, where it formed 30% of the nodules, and the best in mollisol soil, where it formed 70% of the nodules. The most successful competitor for nodulation in multistrain inocula was strain TAL1145, which outcompeted native and other inoculum Leucaena rhizobia in both soils. None of the strains in single or multistrain inoculants was capable of completely overcoming the resident rhizobia, which formed 4 to 70% of the total nodules in oxisol soil and 12 to 72% in mollisol soil. No strong relationship was detected between the size of the rhizosphere population of a strain and its successful occupation of nodules.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Belser L. W., Schmidt E. L. Serological diversity within a terrestrial ammonia-oxidizing population. Appl Environ Microbiol. 1978 Oct;36(4):589–593. doi: 10.1128/aem.36.4.589-593.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bohlool B. B., Schmidt E. L. Nonspecific staining: its control in immunofluorescence examination of soil. Science. 1968 Nov 29;162(3857):1012–1014. doi: 10.1126/science.162.3857.1012. [DOI] [PubMed] [Google Scholar]
- Kingsley M. T., Bohlool B. B. Release of Rhizobium spp. from Tropical Soils and Recovery for Immunofluorescence Enumeration. Appl Environ Microbiol. 1981 Aug;42(2):241–248. doi: 10.1128/aem.42.2.241-248.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- May S. N., Bohlool B. B. Competition Among Rhizobium leguminosarum Strains for Nodulation of Lentils (Lens esculenta). Appl Environ Microbiol. 1983 Mar;45(3):960–965. doi: 10.1128/aem.45.3.960-965.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moawad H. A., Ellis W. R., Schmidt E. L. Rhizosphere Response as a Factor in Competition Among Three Serogroups of Indigenous Rhizobium japonicum for Nodulation of Field-Grown Soybeans. Appl Environ Microbiol. 1984 Apr;47(4):607–612. doi: 10.1128/aem.47.4.607-612.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reyes V. G., Schmidt E. L. Population Densities of Rhizobium japonicum Strain 123 Estimated Directly in Soil and Rhizospheres. Appl Environ Microbiol. 1979 May;37(5):854–858. doi: 10.1128/aem.37.5.854-858.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robert F. M., Schmidt E. L. Population Changes and Persistence of Rhizobium phaseoli in Soil and Rhizospheres. Appl Environ Microbiol. 1983 Feb;45(2):550–556. doi: 10.1128/aem.45.2.550-556.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schmidt E. L., Bakole R. O., Bohlool B. B. Fluorescent-antibody approach to study of rhizobia in soil. J Bacteriol. 1968 Jun;95(6):1987–1992. doi: 10.1128/jb.95.6.1987-1992.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]