Abstract
We attempted to shorten the required time for enrichment broth culture for the isolation of Campylobacter jejuni. Enrichment broths described by Doyle and Roman and Park and Stankiewicz and one developed during this study were compared for ability to isolate C. jejuni from raw chicken carcasses. Our medium was a modification of that of Doyle and Roman with the addition of filter-sterilized FBP (0.2% ferrous sulfate, 0.025% sodium metabisulfite, 0.05% sodium pyruvate), 0.1% sodium lauryl sulfate, and 0.075% agar. Initially, laboratory strains were employed in the development of this medium. Subsequently, an indigenous load of C. jejuni obtained from chickens was used to compare media. Isolation rate comparisons were as follows: direct plating, 40%; Doyle and Roman broth, 45% at 7 h and 61% at 16 h; Park and Stankiewicz broth, 53% at 7 h and 60% at 16 h; our broth, 48% at 7 h and 50% at 16 h. In addition to having the highest isolation rate, the enrichment broth of Doyle and Roman showed greatest selectivity. Our inoculation method of indigenous bacteria provided a controlled means for comparison of isolation procedures.
Full text
PDF


Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Doyle M. P., Roman D. J. Recovery of Campylobacter jejuni and Campylobacter coli from inoculated foods by selective enrichment. Appl Environ Microbiol. 1982 Jun;43(6):1343–1353. doi: 10.1128/aem.43.6.1343-1353.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- George H. A., Hoffman P. S., Smibert R. M., Krieg N. R. Improved media for growth and aerotolerance of Campylobacter fetus. J Clin Microbiol. 1978 Jul;8(1):36–41. doi: 10.1128/jcm.8.1.36-41.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pai C. H., Sorger S., Lackman L., Sinai R. E., Marks M. I. Campylobacter gastroenteritis in children. J Pediatr. 1979 Apr;94(4):589–591. doi: 10.1016/s0022-3476(79)80016-5. [DOI] [PubMed] [Google Scholar]
- Skirrow M. B. Campylobacter enteritis: a "new" disease. Br Med J. 1977 Jul 2;2(6078):9–11. doi: 10.1136/bmj.2.6078.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stern N. J., Kotula A. W. Survival of Campylobacter jejuni inoculated into ground beef. Appl Environ Microbiol. 1982 Nov;44(5):1150–1153. doi: 10.1128/aem.44.5.1150-1153.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Svedhem A., Kaijser B. Campylobacter fetus subspecies jejuni: a common cause of diarrhea in Sweden. J Infect Dis. 1980 Sep;142(3):353–359. doi: 10.1093/infdis/142.3.353. [DOI] [PubMed] [Google Scholar]