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. 1984 Jul;48(1):199–205. doi: 10.1128/aem.48.1.199-205.1984

Interaction of Pseudomonas solanacearum with Suspension-Cultured Tobacco Cells and Tobacco Leaf Cell Walls In Vitro

Jonathan P Duvick 1,†,*, Luis Sequeira 1
PMCID: PMC240366  PMID: 16346588

Abstract

Attachment of radiolabeled Pseudomonas solanacearum cells to suspension-cultured tobacco cells and tobacco leaf cell walls was measured in vitro by a filtration technique that allowed separation of attached and unattached bacteria. An avirulent strain (B1) attached more rapidly to suspension-cultured cells than did the virulent parent strain (K60), and B1 attachment was less sensitive to inhibition by high ionic strength than was K60. Attachment of B1 bacteria to suspension-cultured cells and to leaf cell walls was comparable (50 to 70%), but only a small proportion (10 to 20%) of K60 bacteria attached to leaf cell walls under optimal conditions. With high bacterial populations (108 bacteria per ml), attachment of K60 to suspension-cultured cells was greatly reduced. Attachment of both strains was completely inhibited by pretreating bacterial cells with heat (41°C) or azide and was partially inhibited by EDTA and kanamycin. The mechanism of attachment is not known, but ionic forces may be involved.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Douglas C. J., Halperin W., Nester E. W. Agrobacterium tumefaciens mutants affected in attachment to plant cells. J Bacteriol. 1982 Dec;152(3):1265–1275. doi: 10.1128/jb.152.3.1265-1275.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Duvick J. P., Sequeira L. Interaction of Pseudomonas solanacearum Lipopolysaccharide and Extracellular Polysaccharide with Agglutinin from Potato Tubers. Appl Environ Microbiol. 1984 Jul;48(1):192–198. doi: 10.1128/aem.48.1.192-198.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gubish E. R., Jr, Mace M. L., Jr, Steiner S. M., Williams R. P. Assessment of attachment of Neisseria gonorrhoeae to HeLa cells by double radiolabeling. Infect Immun. 1979 Sep;25(3):1043–1050. doi: 10.1128/iai.25.3.1043-1050.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Heckels J. E., Blackett B., Everson J. S., Ward M. E. The influence of surface charge on the attachment of Neisseria gonorrhoeae to human cells. J Gen Microbiol. 1976 Oct;96(2):359–364. doi: 10.1099/00221287-96-2-359. [DOI] [PubMed] [Google Scholar]
  5. Kotarski S. F., Savage D. C. Models for study of the specificity by which indigenous lactobacilli adhere to murine gastric epithelia. Infect Immun. 1979 Dec;26(3):966–975. doi: 10.1128/iai.26.3.966-975.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Leach J. E., Cantrell M. A., Sequeira L. Hydroxyproline-rich bacterial agglutinin from potato : extraction, purification, and characterization. Plant Physiol. 1982 Nov;70(5):1353–1358. doi: 10.1104/pp.70.5.1353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lippincott B. B., Whatley M. H., Lippincott J. A. Tumor induction by agrobacterium involves attachment of the bacterium to a site on the host plant cell wall. Plant Physiol. 1977 Mar;59(3):388–390. doi: 10.1104/pp.59.3.388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Matthysse A. G., Holmes K. V., Gurlitz R. H. Elaboration of cellulose fibrils by Agrobacterium tumefaciens during attachment to carrot cells. J Bacteriol. 1981 Jan;145(1):583–595. doi: 10.1128/jb.145.1.583-595.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mellon J. E., Helgeson J. P. Interaction of a hydroxyproline-rich glycoprotein from tobacco callus with potential pathogens. Plant Physiol. 1982 Aug;70(2):401–405. doi: 10.1104/pp.70.2.401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ohyama K., Pelcher L. E., Schaefer A. In Vitro Binding of Agrobacterium tumefaciens to Plant Cells from Suspension Culture. Plant Physiol. 1979 Feb;63(2):382–387. doi: 10.1104/pp.63.2.382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sing V. O., Schroth M. N. Bacteria--plant cell surface interactions: active immobilization of saprophytic bacteria in plant leaves. Science. 1977 Aug 19;197(4305):759–761. doi: 10.1126/science.197.4305.759. [DOI] [PubMed] [Google Scholar]
  12. Whatley M. H., Hunter N., Cantrell M. A., Hendrick C., Keegstra K., Sequeira L. Lipopolysaccharide Composition of the Wilt Pathogen, Pseudomonas solanacearum: CORRELATION WITH THE HYPERSENSITIVE RESPONSE IN TOBACCO. Plant Physiol. 1980 Mar;65(3):557–559. doi: 10.1104/pp.65.3.557. [DOI] [PMC free article] [PubMed] [Google Scholar]

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