Abstract
Competitive polymerase chain reaction was used to quantitate latent varicella-zoster virus (VZV) DNA in human trigeminal ganglia. Ganglionic DNA from five subjects was amplified with oligonucleotide primers specific for VZV gene 28. Two of the samples were also analyzed with primers specific for VZV gene 62. Our results indicated that there are 6 to 31 copies of the VZV genome in every 100,000 ganglionic cells.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Davison A. J., Scott J. E. The complete DNA sequence of varicella-zoster virus. J Gen Virol. 1986 Sep;67(Pt 9):1759–1816. doi: 10.1099/0022-1317-67-9-1759. [DOI] [PubMed] [Google Scholar]
- Efstathiou S., Minson A. C., Field H. J., Anderson J. R., Wildy P. Detection of herpes simplex virus-specific DNA sequences in latently infected mice and in humans. J Virol. 1986 Feb;57(2):446–455. doi: 10.1128/jvi.57.2.446-455.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Forghani B., Dupuis K. W., Schmidt N. J. Varicella-zoster viral glycoproteins analyzed with monoclonal antibodies. J Virol. 1984 Oct;52(1):55–62. doi: 10.1128/jvi.52.1.55-62.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gilden D. H., Dueland A. N., Devlin M. E., Mahalingam R., Cohrs R. Varicella-zoster virus reactivation without rash. J Infect Dis. 1992 Aug;166 (Suppl 1):S30–S34. doi: 10.1093/infdis/166.supplement_1.s30. [DOI] [PubMed] [Google Scholar]
- Gilliland G., Perrin S., Blanchard K., Bunn H. F. Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Proc Natl Acad Sci U S A. 1990 Apr;87(7):2725–2729. doi: 10.1073/pnas.87.7.2725. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOPE-SIMPSON R. E. THE NATURE OF HERPES ZOSTER: A LONG-TERM STUDY AND A NEW HYPOTHESIS. Proc R Soc Med. 1965 Jan;58:9–20. doi: 10.1177/003591576505800106. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mahalingam R., Wellish M. C., Dueland A. N., Cohrs R. J., Gilden D. H. Localization of herpes simplex virus and varicella zoster virus DNA in human ganglia. Ann Neurol. 1992 Apr;31(4):444–448. doi: 10.1002/ana.410310417. [DOI] [PubMed] [Google Scholar]
- Mahalingam R., Wellish M., Wolf W., Dueland A. N., Cohrs R., Vafai A., Gilden D. Latent varicella-zoster viral DNA in human trigeminal and thoracic ganglia. N Engl J Med. 1990 Sep 6;323(10):627–631. doi: 10.1056/NEJM199009063231002. [DOI] [PubMed] [Google Scholar]
- Saiki R. K., Scharf S., Faloona F., Mullis K. B., Horn G. T., Erlich H. A., Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350–1354. doi: 10.1126/science.2999980. [DOI] [PubMed] [Google Scholar]