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. 1991 Jan;65(1):499–504. doi: 10.1128/jvi.65.1.499-504.1991

The 32-kilodalton envelope protein of vaccinia virus synthesized in Escherichia coli binds with specificity to cell surfaces.

C F Lai 1, S C Gong 1, M Esteban 1
PMCID: PMC240546  PMID: 1985213

Abstract

The nature of interaction between vaccinia virus and the surface of host cells as the first step in virus infection is undefined. A 32-kDa virus envelope protein has been identified as a cell surface binding protein (J.-S. Maa, J. F. Rodriguez, and M. Esteban, J. Biol. Chem. 265:1569-1577, 1990). To carry out studies on the structure-function relationship of this protein, the 32-kDa protein was obtained from Escherichia coli cells harboring the expression plasmid pT7Ek32. The recombinant polypeptide was found to have structural properties similar to those of the native virus envelope protein. Binding studies of 125I-labeled 32-kDa protein to cultured cells of various origins revealed that the E. coli-produced 32-kDa protein exhibited selectivity, specificity, and saturability. Scatchard analysis indicated about 4.5 x 10(4) sites per cell with a high affinity (Kd = 1.8 x 10(-9) M), suggesting interaction of the 32-kDa protein with a specific receptor. The availability of large quantities of the 32-kDa virus protein in bacteria will permit further structural and functional studies of this virus envelope protein and facilitate identification of the specific cell surface receptor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong J. A., Metz D. H., Young M. R. The mode of entry of vaccinia virus into L cells. J Gen Virol. 1973 Dec;21(3):533–537. doi: 10.1099/0022-1317-21-3-533. [DOI] [PubMed] [Google Scholar]
  2. Clayson E. T., Compans R. W. Entry of simian virus 40 is restricted to apical surfaces of polarized epithelial cells. Mol Cell Biol. 1988 Aug;8(8):3391–3396. doi: 10.1128/mcb.8.8.3391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Co M. S., Gaulton G. N., Fields B. N., Greene M. I. Isolation and biochemical characterization of the mammalian reovirus type 3 cell-surface receptor. Proc Natl Acad Sci U S A. 1985 Mar;82(5):1494–1498. doi: 10.1073/pnas.82.5.1494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Colonno R. J., Condra J. H., Mizutani S., Callahan P. L., Davies M. E., Murcko M. A. Evidence for the direct involvement of the rhinovirus canyon in receptor binding. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5449–5453. doi: 10.1073/pnas.85.15.5449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DALES S., KAJIOKA R. THE CYCLE OF MULTIPLICATION OF VACCINIA VIRUS IN EARLE'S STRAIN L CELLS. I. UPTAKE AND PENETRATION. Virology. 1964 Nov;24:278–294. doi: 10.1016/0042-6822(64)90167-9. [DOI] [PubMed] [Google Scholar]
  6. Dales S. Early events in cell-animal virus interactions. Bacteriol Rev. 1973 Jun;37(2):103–135. doi: 10.1128/br.37.2.103-135.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dalgleish A. G., Beverley P. C., Clapham P. R., Crawford D. H., Greaves M. F., Weiss R. A. The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus. Nature. 1984 Dec 20;312(5996):763–767. doi: 10.1038/312763a0. [DOI] [PubMed] [Google Scholar]
  8. Dimmock N. J. Review article initial stages in infection with animal viruses. J Gen Virol. 1982 Mar;59(Pt 1):1–22. doi: 10.1099/0022-1317-59-1-1. [DOI] [PubMed] [Google Scholar]
  9. Eppstein D. A., Marsh Y. V., Schreiber A. B., Newman S. R., Todaro G. J., Nestor J. J., Jr Epidermal growth factor receptor occupancy inhibits vaccinia virus infection. Nature. 1985 Dec 19;318(6047):663–665. doi: 10.1038/318663a0. [DOI] [PubMed] [Google Scholar]
  10. Essani K., Dales S. Biogenesis of vaccinia: evidence for more than 100 polypeptides in the virion. Virology. 1979 Jun;95(2):385–394. doi: 10.1016/0042-6822(79)90493-8. [DOI] [PubMed] [Google Scholar]
  11. Fingeroth J. D., Weis J. J., Tedder T. F., Strominger J. L., Biro P. A., Fearon D. T. Epstein-Barr virus receptor of human B lymphocytes is the C3d receptor CR2. Proc Natl Acad Sci U S A. 1984 Jul;81(14):4510–4514. doi: 10.1073/pnas.81.14.4510. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gong S. C., Lai C. F., Esteban M. Vaccinia virus induces cell fusion at acid pH and this activity is mediated by the N-terminus of the 14-kDa virus envelope protein. Virology. 1990 Sep;178(1):81–91. doi: 10.1016/0042-6822(90)90381-z. [DOI] [PubMed] [Google Scholar]
  13. Greve J. M., Davis G., Meyer A. M., Forte C. P., Yost S. C., Marlor C. W., Kamarck M. E., McClelland A. The major human rhinovirus receptor is ICAM-1. Cell. 1989 Mar 10;56(5):839–847. doi: 10.1016/0092-8674(89)90688-0. [DOI] [PubMed] [Google Scholar]
  14. Herz R. E., Ojakian G. K. Differential targeting of an epithelial plasma membrane glycoprotein in polarized Madin-Darby canine kidney cells. J Biol Chem. 1989 Mar 15;264(8):4605–4612. [PubMed] [Google Scholar]
  15. Ichihashi Y., Oie M. Adsorption and penetration of the trypsinized vaccinia virion. Virology. 1980 Feb;101(1):50–60. doi: 10.1016/0042-6822(80)90482-1. [DOI] [PubMed] [Google Scholar]
  16. Johnson D. C., Burke R. L., Gregory T. Soluble forms of herpes simplex virus glycoprotein D bind to a limited number of cell surface receptors and inhibit virus entry into cells. J Virol. 1990 Jun;64(6):2569–2576. doi: 10.1128/jvi.64.6.2569-2576.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kaner R. J., Baird A., Mansukhani A., Basilico C., Summers B. D., Florkiewicz R. Z., Hajjar D. P. Fibroblast growth factor receptor is a portal of cellular entry for herpes simplex virus type 1. Science. 1990 Jun 15;248(4961):1410–1413. doi: 10.1126/science.2162560. [DOI] [PubMed] [Google Scholar]
  18. Lasky L. A., Nakamura G., Smith D. H., Fennie C., Shimasaki C., Patzer E., Berman P., Gregory T., Capon D. J. Delineation of a region of the human immunodeficiency virus type 1 gp120 glycoprotein critical for interaction with the CD4 receptor. Cell. 1987 Sep 11;50(6):975–985. doi: 10.1016/0092-8674(87)90524-1. [DOI] [PubMed] [Google Scholar]
  19. Lentz T. L., Burrage T. G., Smith A. L., Crick J., Tignor G. H. Is the acetylcholine receptor a rabies virus receptor? Science. 1982 Jan 8;215(4529):182–184. doi: 10.1126/science.7053569. [DOI] [PubMed] [Google Scholar]
  20. Maa J. S., Rodriguez J. F., Esteban M. Structural and functional characterization of a cell surface binding protein of vaccinia virus. J Biol Chem. 1990 Jan 25;265(3):1569–1577. [PubMed] [Google Scholar]
  21. Moss B., Flexner C. Vaccinia virus expression vectors. Annu Rev Immunol. 1987;5:305–324. doi: 10.1146/annurev.iy.05.040187.001513. [DOI] [PubMed] [Google Scholar]
  22. Niles E. G., Seto J. Vaccinia virus gene D8 encodes a virion transmembrane protein. J Virol. 1988 Oct;62(10):3772–3778. doi: 10.1128/jvi.62.10.3772-3778.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Oie M., Ichihashi Y. Modification of vaccinia virus penetration proteins analyzed by monoclonal antibodies. Virology. 1987 Apr;157(2):449–459. doi: 10.1016/0042-6822(87)90287-x. [DOI] [PubMed] [Google Scholar]
  24. Rodriguez J. F., Paez E., Esteban M. A 14,000-Mr envelope protein of vaccinia virus is involved in cell fusion and forms covalently linked trimers. J Virol. 1987 Feb;61(2):395–404. doi: 10.1128/jvi.61.2.395-404.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Stern W., Dales S. Biogenesis of vaccinia: isolation and characterization of a surface component that elicits antibody suppressing infectivity and cell-cell fusion. Virology. 1976 Nov;75(1):232–241. doi: 10.1016/0042-6822(76)90022-2. [DOI] [PubMed] [Google Scholar]
  26. Tardieu M., Epstein R. L., Weiner H. L. Interaction of viruses with cell surface receptors. Int Rev Cytol. 1982;80:27–61. doi: 10.1016/S0074-7696(08)60366-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Taylor H. P., Cooper N. R. Human cytomegalovirus binding to fibroblasts is receptor mediated. J Virol. 1989 Sep;63(9):3991–3998. doi: 10.1128/jvi.63.9.3991-3998.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Verdin E. M., King G. L., Maratos-Flier E. Characterization of a common high-affinity receptor for reovirus serotypes 1 and 3 on endothelial cells. J Virol. 1989 Mar;63(3):1318–1325. doi: 10.1128/jvi.63.3.1318-1325.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. White J. M., Littman D. R. Viral receptors of the immunoglobulin superfamily. Cell. 1989 Mar 10;56(5):725–728. doi: 10.1016/0092-8674(89)90674-0. [DOI] [PubMed] [Google Scholar]

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