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. 1991 May;65(5):2699–2701. doi: 10.1128/jvi.65.5.2699-2701.1991

Formation and selection of intergenogroup reassortants during cell culture adaptation of rotaviruses from dually infected subjects.

R L Ward 1, O Nakagomi 1, D R Knowlton 1, M M McNeal 1, T Nakagomi 1, N Huda 1, J D Clemens 1, D A Sack 1
PMCID: PMC240631  PMID: 1850042

Abstract

A previous study showed that intergenogroup reassortants of human rotaviruses can persist in nature (R.L. Ward, O. Nakagomi, D.R. Knowlton, M.M. McNeal, T. Nakagomi, J.D. Clemens, D.A. Sack, and G.M. Schiff, J. Virol. 64:3219-3225, 1990), but the mechanisms involved in their formation and selection had not been determined. In this study it was shown that, during cell culture adaptation of rotaviruses belonging to different genogroups from stools of dually infected subjects, intergenogroup reassortants were formed and selected, presumably mimicking the processes that occur in nature.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Estes M. K., Cohen J. Rotavirus gene structure and function. Microbiol Rev. 1989 Dec;53(4):410–449. doi: 10.1128/mr.53.4.410-449.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Greenberg H. B., Flores J., Kalica A. R., Wyatt R. G., Jones R. Gene coding assignments for growth restriction, neutralization and subgroup specificities of the W and DS-1 strains of human rotavirus. J Gen Virol. 1983 Feb;64(Pt 2):313–320. doi: 10.1099/0022-1317-64-2-313. [DOI] [PubMed] [Google Scholar]
  3. Hoshino Y., Sereno M. M., Midthun K., Flores J., Kapikian A. Z., Chanock R. M. Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proc Natl Acad Sci U S A. 1985 Dec;82(24):8701–8704. doi: 10.1073/pnas.82.24.8701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kalica A. R., Greenberg H. B., Espejo R. T., Flores J., Wyatt R. G., Kapikian A. Z., Chanock R. M. Distinctive ribonucleic acid patterns of human rotavirus subgroups 1 and 2. Infect Immun. 1981 Sep;33(3):958–961. doi: 10.1128/iai.33.3.958-961.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kutsuzawa T., Konno T., Suzuki H., Ebina T., Ishida N. Two distinct electrophoretic migration patterns of RNA segments of human rotaviruses prevalent in Japan in relation to their serotypes. Microbiol Immunol. 1982;26(3):271–273. doi: 10.1111/j.1348-0421.1982.tb00177.x. [DOI] [PubMed] [Google Scholar]
  6. Nakagomi O., Nakagomi T., Akatani K., Ikegami N. Identification of rotavirus genogroups by RNA-RNA hybridization. Mol Cell Probes. 1989 Sep;3(3):251–261. doi: 10.1016/0890-8508(89)90006-6. [DOI] [PubMed] [Google Scholar]
  7. Nakagomi T., Nakagomi O. RNA-RNA hybridization identifies a human rotavirus that is genetically related to feline rotavirus. J Virol. 1989 Mar;63(3):1431–1434. doi: 10.1128/jvi.63.3.1431-1434.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Offit P. A., Blavat G. Identification of the two rotavirus genes determining neutralization specificities. J Virol. 1986 Jan;57(1):376–378. doi: 10.1128/jvi.57.1.376-378.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ward R. L., Knowlton D. R. Genotypic selection following coinfection of cultured cells with subgroup 1 and subgroup 2 human rotaviruses. J Gen Virol. 1989 Jul;70(Pt 7):1691–1699. doi: 10.1099/0022-1317-70-7-1691. [DOI] [PubMed] [Google Scholar]
  10. Ward R. L., Knowlton D. R., Hurst P. F. Reassortant formation and selection following coinfection of cultured cells with subgroup 2 human rotaviruses. J Gen Virol. 1988 Jan;69(Pt 1):149–162. doi: 10.1099/0022-1317-69-1-149. [DOI] [PubMed] [Google Scholar]
  11. Ward R. L., Knowlton D. R., Pierce M. J. Efficiency of human rotavirus propagation in cell culture. J Clin Microbiol. 1984 Jun;19(6):748–753. doi: 10.1128/jcm.19.6.748-753.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ward R. L., Nakagomi O., Knowlton D. R., McNeal M. M., Nakagomi T., Clemens J. D., Sack D. A., Schiff G. M. Evidence for natural reassortants of human rotaviruses belonging to different genogroups. J Virol. 1990 Jul;64(7):3219–3225. doi: 10.1128/jvi.64.7.3219-3225.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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