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. 1991 May;65(5):2702–2706. doi: 10.1128/jvi.65.5.2702-2706.1991

Assembly of empty capsids by using baculovirus recombinants expressing human parvovirus B19 structural proteins.

C S Brown 1, J W Van Lent 1, J M Vlak 1, W J Spaan 1
PMCID: PMC240632  PMID: 1901917

Abstract

Empty parvovirus B19 capsids were isolated from insect cells infected with a recombinant baculovirus expressing parvovirus B19 VP2 alone and also with a double-recombinant baculovirus expressing both VP1 and VP2. That VP2 alone can assemble to form capsids is a phenomenon not previously observed in parvoviruses. The stoichiometry of the capsids containing both VP1 and VP2 was similar to that previously observed in parvovirus B19-infected cells. The capsids were similar to native capsids in size and appearance, and their antigenicity was demonstrated by immunoprecipitation and enzyme-linked immunosorbent assay with B19-specific antibodies.

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