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. 1991 May;65(5):2714–2717. doi: 10.1128/jvi.65.5.2714-2717.1991

Encephalomyocarditis virus RNA polymerase preparations, with and without RNA helicase activity.

T M Dmitrieva 1, K B Norkina 1, V I Agol 1
PMCID: PMC240635  PMID: 1850044

Abstract

RNA template- and primer-dependent preparations of RNA polymerase were purified from encephalomyocarditis virus-infected Krebs-2 cells, using a three-step chromatographic procedure. The RNA duplex-unwinding activity of these preparations was investigated by two assays, using a partially double-stranded RNA template (encephalomyocarditis virus RNA annealed with a long segment of antisense transcript). Less purified preparations of the polymerase appeared to be able to efficiently displace, in an ATP-dependent and RNA elongation-dependent reaction, the antisense segment from the template. However, upon a more extensive purification, the unwinding activity of the RNA polymerase preparations was lost.

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Selected References

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