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. 1992 Feb;66(2):1273–1276. doi: 10.1128/jvi.66.2.1273-1276.1992

Replication of parvovirus B19 in hematopoietic progenitor cells generated in vitro from normal human peripheral blood.

T F Schwarz 1, S Serke 1, B Hottenträger 1, A von Brunn 1, H Baurmann 1, A Kirsch 1, W Stolz 1, D Huhn 1, F Deinhardt 1, M Roggendorf 1
PMCID: PMC240841  PMID: 1731104

Abstract

Erythroid progenitor cells generated in vitro from peripheral human blood in the presence of interleukin-3 and erythropoietin were infected with human parvovirus B19. B19 virus DNA replication was highest 48 to 72 h after infection, and maximum levels of B19 virus proteins were detected in culture supernatants at 72 to 96 h after infection. B19 virus propagated in vitro was infectious. This cell culture system with peripheral blood cells facilitates studies in vitro of B19 virus replication.

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Selected References

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  1. Anderson M. J., Lewis E., Kidd I. M., Hall S. M., Cohen B. J. An outbreak of erythema infectiosum associated with human parvovirus infection. J Hyg (Lond) 1984 Aug;93(1):85–93. doi: 10.1017/s0022172400060964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cohen B. J., Mortimer P. P., Pereira M. S. Diagnostic assays with monoclonal antibodies for the human serum parvovirus-like virus (SPLV). J Hyg (Lond) 1983 Aug;91(1):113–130. doi: 10.1017/s0022172400060095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cossart Y. E., Field A. M., Cant B., Widdows D. Parvovirus-like particles in human sera. Lancet. 1975 Jan 11;1(7898):72–73. doi: 10.1016/s0140-6736(75)91074-0. [DOI] [PubMed] [Google Scholar]
  4. Frickhofen N., Abkowitz J. L., Safford M., Berry J. M., Antunez-de-Mayolo J., Astrow A., Cohen R., Halperin I., King L., Mintzer D. Persistent B19 parvovirus infection in patients infected with human immunodeficiency virus type 1 (HIV-1): a treatable cause of anemia in AIDS. Ann Intern Med. 1990 Dec 15;113(12):926–933. doi: 10.7326/0003-4819-113-12-926. [DOI] [PubMed] [Google Scholar]
  5. Kurtzman G. J., Cohen B., Meyers P., Amunullah A., Young N. S. Persistent B19 parvovirus infection as a cause of severe chronic anaemia in children with acute lymphocytic leukaemia. Lancet. 1988 Nov 19;2(8621):1159–1162. doi: 10.1016/s0140-6736(88)90233-4. [DOI] [PubMed] [Google Scholar]
  6. Kurtzman G., Frickhofen N., Kimball J., Jenkins D. W., Nienhuis A. W., Young N. S. Pure red-cell aplasia of 10 years' duration due to persistent parvovirus B19 infection and its cure with immunoglobulin therapy. N Engl J Med. 1989 Aug 24;321(8):519–523. doi: 10.1056/NEJM198908243210807. [DOI] [PubMed] [Google Scholar]
  7. Mortimer P. P., Humphries R. K., Moore J. G., Purcell R. H., Young N. S. A human parvovirus-like virus inhibits haematopoietic colony formation in vitro. 1983 Mar 31-Apr 6Nature. 302(5907):426–429. doi: 10.1038/302426a0. [DOI] [PubMed] [Google Scholar]
  8. Ozawa K., Kurtzman G., Young N. Replication of the B19 parvovirus in human bone marrow cell cultures. Science. 1986 Aug 22;233(4766):883–886. doi: 10.1126/science.3738514. [DOI] [PubMed] [Google Scholar]
  9. Ozawa K., Young N. Characterization of capsid and noncapsid proteins of B19 parvovirus propagated in human erythroid bone marrow cell cultures. J Virol. 1987 Aug;61(8):2627–2630. doi: 10.1128/jvi.61.8.2627-2630.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Potter C. G., Potter A. C., Hatton C. S., Chapel H. M., Anderson M. J., Pattison J. R., Tyrrell D. A., Higgins P. G., Willman J. S., Parry H. F. Variation of erythroid and myeloid precursors in the marrow and peripheral blood of volunteer subjects infected with human parvovirus (B19). J Clin Invest. 1987 May;79(5):1486–1492. doi: 10.1172/JCI112978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schwarz T. F., Nerlich A., Hottenträger B., Jäger G., Wiest I., Kantimm S., Roggendorf H., Schultz M., Gloning K. P., Schramm T. Parvovirus B19 infection of the fetus. Histology and in situ hybridization. Am J Clin Pathol. 1991 Jul;96(1):121–126. doi: 10.1093/ajcp/96.1.121. [DOI] [PubMed] [Google Scholar]
  12. Schwarz T. F., Roggendorf M., Deinhardt F. Human parvovirus B19: ELISA and immunoblot assays. J Virol Methods. 1988 Jun;20(2):155–168. doi: 10.1016/0166-0934(88)90149-8. [DOI] [PubMed] [Google Scholar]
  13. Schwarz T. F., Roggendorf M., Hottenträger B., Modrow S., Deinhardt F., Middeldorp J. Immunoglobulins in the prophylaxis of parvovirus B19 infection. J Infect Dis. 1990 Nov;162(5):1214–1214. doi: 10.1093/infdis/162.5.1214. [DOI] [PubMed] [Google Scholar]
  14. Serjeant G. R., Topley J. M., Mason K., Serjeant B. E., Pattison J. R., Jones S. E., Mohamed R. Outbreak of aplastic crises in sickle cell anaemia associated with parvovirus-like agent. Lancet. 1981 Sep 19;2(8247):595–597. doi: 10.1016/s0140-6736(81)92739-2. [DOI] [PubMed] [Google Scholar]
  15. Serke S., Schwarz T. F., Baurmann H., Kirsch A., Hottenträger B., Von Brunn A., Roggendorf M., Huhn D., Deinhardt F. Productive infection of in vitro generated haemopoietic progenitor cells from normal human adult peripheral blood with parvovirus B19: studies by morphology, immunocytochemistry, flow-cytometry and DNA-hybridization. Br J Haematol. 1991 Sep;79(1):6–13. doi: 10.1111/j.1365-2141.1991.tb07999.x. [DOI] [PubMed] [Google Scholar]
  16. Serke S., Säuberlich S., Huhn D. A liquid culture method for the in vitro growth of hemopoietic progenitor cells from normal human adult peripheral blood allowing for analysis by multiparameter flow-cytometry. Eur J Haematol. 1991 Feb;46(2):85–92. doi: 10.1111/j.1600-0609.1991.tb00527.x. [DOI] [PubMed] [Google Scholar]
  17. Srivastava A., Lu L. Replication of B19 parvovirus in highly enriched hematopoietic progenitor cells from normal human bone marrow. J Virol. 1988 Aug;62(8):3059–3063. doi: 10.1128/jvi.62.8.3059-3063.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Takahashi T., Ozawa K., Mitani K., Miyazono K., Asano S., Takaku F. B19 parvovirus replicates in erythroid leukemic cells in vitro. J Infect Dis. 1989 Sep;160(3):548–549. doi: 10.1093/infdis/160.3.548. [DOI] [PubMed] [Google Scholar]
  19. Takahashi T., Ozawa K., Takahashi K., Asano S., Takaku F. Susceptibility of human erythropoietic cells to B19 parvovirus in vitro increases with differentiation. Blood. 1990 Feb 1;75(3):603–610. [PubMed] [Google Scholar]
  20. Yaegashi N., Shiraishi H., Takeshita T., Nakamura M., Yajima A., Sugamura K. Propagation of human parvovirus B19 in primary culture of erythroid lineage cells derived from fetal liver. J Virol. 1989 Jun;63(6):2422–2426. doi: 10.1128/jvi.63.6.2422-2426.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Young N. S., Mortimer P. P., Moore J. G., Humphries R. K. Characterization of a virus that causes transient aplastic crisis. J Clin Invest. 1984 Jan;73(1):224–230. doi: 10.1172/JCI111195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Young N., Harrison M., Moore J., Mortimer P., Humphries R. K. Direct demonstration of the human parvovirus in erythroid progenitor cells infected in vitro. J Clin Invest. 1984 Dec;74(6):2024–2032. doi: 10.1172/JCI111625. [DOI] [PMC free article] [PubMed] [Google Scholar]

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