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. 1992 Mar;66(3):1432–1441. doi: 10.1128/jvi.66.3.1432-1441.1992

Functional characterization of a U5 ribozyme: intracellular suppression of human immunodeficiency virus type 1 expression.

B Dropulić 1, N H Lin 1, M A Martin 1, K T Jeang 1
PMCID: PMC240867  PMID: 1738200

Abstract

We have designed a ribozyme that cleaves human immunodeficiency virus type 1 (HIV-1) RNA in U5 (at nucleotide +115). This ribozyme was tested in vitro and was found to give efficient and specific digestion of RNA containing the HIV-1 U5 sequence. When the U5 ribozyme was placed into the HIV-1 genome, virus replication was suppressed in tissue culture. Introduction of this ribozyme into cells by using an amphotropic retrovirus vector significantly reduced expression of U5-containing RNA in cells chronically infected with HIV-1. Naive T cells were cocultivated with packaging cells that produce defective amphotropic retroviruses containing the U5 ribozyme. These lymphocytes were found to be partially protected from HIV-1 infection.

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