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. 2000 Oct 26;83(10):1309–1317. doi: 10.1054/bjoc.2000.1458

Molecular cytogenetic analysis of breast cancer cell lines

J M Davidson 1, K L Gorringe 2, S-F Chin 2, B Orsetti 3, C Besret 1, C Courtay-Cahen 1, I Roberts 1, C Theillet 3, C Caldas 2, P A W Edwards 1
PMCID: PMC2408781  PMID: 11044355

Abstract

The extensive chromosome rearrangements of breast carcinomas must contribute to tumour development, but have been largely intractable to classical cytogenetic banding. We report here the analysis by 24-colour karyotyping and comparative genomic hybridization (CGH) of 19 breast carcinoma cell lines and one normal breast epithelial cell line, which provide model examples of karyotype patterns and translocations present in breast carcinomas. The CGH was compared with CGH of 106 primary breast cancers. The lines varied from perfectly diploid to highly aneuploid. Translocations were very varied and over 98% were unbalanced. The most frequent in the carcinomas were 8;11 in five lines; and 8;17, 1;4 and 1;10 in four lines. The most frequently involved chromosome was 8. Several lines showed complex multiply-translocated chromosomes. The very aneuploid karyotypes appeared to fall into two groups that evolved by different routes: one that steadily lost chromosomes and at one point doubled their entire karyotype; and another that steadily gained chromosomes, together with abnormalities. All karyotypes fell within the range seen in fresh material and CGH confirmed that the lines were broadly representative of fresh tumours. The karyotypes provide a resource for the cataloguing and analysis of translocations in these tumours, accessible at http://www.path.cam.ac.uk/~pawefish. © 2000 Cancer Research Campaign

Keywords: chromosome translocations, karyotypes, fluorescence-in situ hybridization, spectral karyotyping, comparative genomicybridization

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Selected References

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  1. Adeyinka A., Kytola S., Mertens F., Pandis N., Larsson C. Spectral karyotyping and chromosome banding studies of primary breast carcinomas and their lymph node metastases. Int J Mol Med. 2000 Mar;5(3):235–240. doi: 10.3892/ijmm.5.3.235. [DOI] [PubMed] [Google Scholar]
  2. Bautista S., Theillet C. CCND1 and FGFR1 coamplification results in the colocalization of 11q13 and 8p12 sequences in breast tumor nuclei. Genes Chromosomes Cancer. 1998 Aug;22(4):268–277. [PubMed] [Google Scholar]
  3. Bernardino J., Apiou F., Gerbault-Seureau M., Malfoy B., Dutrillaux B. Characterization of recurrent homogeneously staining regions in 72 breast carcinomas. Genes Chromosomes Cancer. 1998 Oct;23(2):100–108. doi: 10.1002/(sici)1098-2264(199810)23:2<100::aid-gcc2>3.0.co;2-6. [DOI] [PubMed] [Google Scholar]
  4. Courjal F., Theillet C. Comparative genomic hybridization analysis of breast tumors with predetermined profiles of DNA amplification. Cancer Res. 1997 Oct 1;57(19):4368–4377. [PubMed] [Google Scholar]
  5. Courtay-Cahen C., Morris J. S., Edwards P. A. Chromosome translocations in breast cancer with breakpoints at 8p12. Genomics. 2000 May 15;66(1):15–25. doi: 10.1006/geno.2000.6178. [DOI] [PubMed] [Google Scholar]
  6. Dutrillaux B., Gerbault-Seureau M., Remvikos Y., Zafrani B., Prieur M. Breast cancer genetic evolution: I. Data from cytogenetics and DNA content. Breast Cancer Res Treat. 1991 Nov;19(3):245–255. doi: 10.1007/BF01961161. [DOI] [PubMed] [Google Scholar]
  7. Dutrillaux B. Pathways of chromosome alteration in human epithelial cancers. Adv Cancer Res. 1995;67:59–82. doi: 10.1016/s0065-230x(08)60710-1. [DOI] [PubMed] [Google Scholar]
  8. Forozan F., Veldman R., Ammerman C. A., Parsa N. Z., Kallioniemi A., Kallioniemi O. P., Ethier S. P. Molecular cytogenetic analysis of 11 new breast cancer cell lines. Br J Cancer. 1999 Dec;81(8):1328–1334. doi: 10.1038/sj.bjc.6695007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gioanni J., Le François D., Zanghellini E., Mazeau C., Ettore F., Lambert J. C., Schneider M., Dutrillaux B. Establishment and characterisation of a new tumorigenic cell line with a normal karyotype derived from a human breast adenocarcinoma. Br J Cancer. 1990 Jul;62(1):8–13. doi: 10.1038/bjc.1990.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hambly R. J., Double J. A., Thompson M. J., Bibby M. C. Establishment and characterisation of new cell lines from human breast tumours initially established as tumour xenografts in NMRI nude mice. Breast Cancer Res Treat. 1997 May;43(3):247–258. doi: 10.1023/a:1005756632293. [DOI] [PubMed] [Google Scholar]
  11. Kallioniemi O. P., Kallioniemi A., Piper J., Isola J., Waldman F. M., Gray J. W., Pinkel D. Optimizing comparative genomic hybridization for analysis of DNA sequence copy number changes in solid tumors. Genes Chromosomes Cancer. 1994 Aug;10(4):231–243. doi: 10.1002/gcc.2870100403. [DOI] [PubMed] [Google Scholar]
  12. Kytölä S., Rummukainen J., Nordgren A., Karhu R., Farnebo F., Isola J., Larsson C. Chromosomal alterations in 15 breast cancer cell lines by comparative genomic hybridization and spectral karyotyping. Genes Chromosomes Cancer. 2000 Jul;28(3):308–317. doi: 10.1002/1098-2264(200007)28:3<308::aid-gcc9>3.0.co;2-b. [DOI] [PubMed] [Google Scholar]
  13. Macville M., Schröck E., Padilla-Nash H., Keck C., Ghadimi B. M., Zimonjic D., Popescu N., Ried T. Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping. Cancer Res. 1999 Jan 1;59(1):141–150. [PubMed] [Google Scholar]
  14. McCallum H. M., Lowther G. W. Long-term culture of primary breast cancer in defined medium. Breast Cancer Res Treat. 1996;39(3):247–259. doi: 10.1007/BF01806153. [DOI] [PubMed] [Google Scholar]
  15. Morris J. S., Carter N. P., Ferguson-Smith M. A., Edwards P. A. Cytogenetic analysis of three breast carcinoma cell lines using reverse chromosome painting. Genes Chromosomes Cancer. 1997 Oct;20(2):120–139. doi: 10.1002/(sici)1098-2264(199710)20:2<120::aid-gcc3>3.0.co;2-5. [DOI] [PubMed] [Google Scholar]
  16. Muleris M., Salmon R. J., Dutrillaux B. Existence of two distinct processes of chromosomal evolution in near-diploid colorectal tumors. Cancer Genet Cytogenet. 1988 May;32(1):43–50. doi: 10.1016/0165-4608(88)90310-x. [DOI] [PubMed] [Google Scholar]
  17. Naundorf H., Fichtner I., Saul G. J., Haensch W., Büttner B. Establishment and characteristics of two new human mammary carcinoma lines serially transplantable in nude mice. J Cancer Res Clin Oncol. 1993;119(11):652–656. doi: 10.1007/BF01215983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Osborne C. K., Hobbs K., Trent J. M. Biological differences among MCF-7 human breast cancer cell lines from different laboratories. Breast Cancer Res Treat. 1987;9(2):111–121. doi: 10.1007/BF01807363. [DOI] [PubMed] [Google Scholar]
  19. Persson K., Pandis N., Mertens F., Borg A., Baldetorp B., Killander D., Isola J. Chromosomal aberrations in breast cancer: a comparison between cytogenetics and comparative genomic hybridization. Genes Chromosomes Cancer. 1999 Jun;25(2):115–122. [PubMed] [Google Scholar]
  20. Roberts I., Wienberg J., Nacheva E., Grace C., Griffin D., Coleman N. Novel method for the production of multiple colour chromosome paints for use in karyotyping by fluorescence in situ hybridisation. Genes Chromosomes Cancer. 1999 Jul;25(3):241–250. doi: 10.1002/(sici)1098-2264(199907)25:3<241::aid-gcc6>3.0.co;2-7. [DOI] [PubMed] [Google Scholar]
  21. Schröck E., du Manoir S., Veldman T., Schoell B., Wienberg J., Ferguson-Smith M. A., Ning Y., Ledbetter D. H., Bar-Am I., Soenksen D. Multicolor spectral karyotyping of human chromosomes. Science. 1996 Jul 26;273(5274):494–497. doi: 10.1126/science.273.5274.494. [DOI] [PubMed] [Google Scholar]
  22. Speicher M. R., Gwyn Ballard S., Ward D. C. Karyotyping human chromosomes by combinatorial multi-fluor FISH. Nat Genet. 1996 Apr;12(4):368–375. doi: 10.1038/ng0496-368. [DOI] [PubMed] [Google Scholar]
  23. Stamps A. C., Davies S. C., Burman J., O'Hare M. J. Analysis of proviral integration in human mammary epithelial cell lines immortalized by retroviral infection with a temperature-sensitive SV40 T-antigen construct. Int J Cancer. 1994 Jun 15;57(6):865–874. doi: 10.1002/ijc.2910570616. [DOI] [PubMed] [Google Scholar]
  24. Telenius H., Pelmear A. H., Tunnacliffe A., Carter N. P., Behmel A., Ferguson-Smith M. A., Nordenskjöld M., Pfragner R., Ponder B. A. Cytogenetic analysis by chromosome painting using DOP-PCR amplified flow-sorted chromosomes. Genes Chromosomes Cancer. 1992 Apr;4(3):257–263. doi: 10.1002/gcc.2870040311. [DOI] [PubMed] [Google Scholar]
  25. Tirkkonen M., Tanner M., Karhu R., Kallioniemi A., Isola J., Kallioniemi O. P. Molecular cytogenetics of primary breast cancer by CGH. Genes Chromosomes Cancer. 1998 Mar;21(3):177–184. [PubMed] [Google Scholar]
  26. Veldman T., Vignon C., Schröck E., Rowley J. D., Ried T. Hidden chromosome abnormalities in haematological malignancies detected by multicolour spectral karyotyping. Nat Genet. 1997 Apr;15(4):406–410. doi: 10.1038/ng0497-406. [DOI] [PubMed] [Google Scholar]
  27. Wang X. Z., Jolicoeur E. M., Conte N., Chaffanet M., Zhang Y., Mozziconacci M. J., Feiner H., Birnbaum D., Pébusque M. J., Ron D. gamma-heregulin is the product of a chromosomal translocation fusing the DOC4 and HGL/NRG1 genes in the MDA-MB-175 breast cancer cell line. Oncogene. 1999 Oct 7;18(41):5718–5721. doi: 10.1038/sj.onc.1202950. [DOI] [PubMed] [Google Scholar]
  28. Whitehead R. H., Bertoncello I., Webber L. M., Pedersen J. S. A new human breast carcinoma cell line (PMC42) with stem cell characteristics. I. Morphologic characterization. J Natl Cancer Inst. 1983 Apr;70(4):649–661. [PubMed] [Google Scholar]
  29. Widmaier R., Wildner G. P., Papsdorf G., Graffi I. Uber eine neue, in vitro unbegrenzt wachsende Zellinie, MaTu, von Mamma-Tumorzellen des Menschen. Arch Geschwulstforsch. 1974;44(1):1–10. [PubMed] [Google Scholar]
  30. Wistuba I. I., Behrens C., Milchgrub S., Syed S., Ahmadian M., Virmani A. K., Kurvari V., Cunningham T. H., Ashfaq R., Minna J. D. Comparison of features of human breast cancer cell lines and their corresponding tumors. Clin Cancer Res. 1998 Dec;4(12):2931–2938. [PubMed] [Google Scholar]

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