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. 2008 Mar 13;149(6):3095–3108. doi: 10.1210/en.2007-0425

Figure 3.

Figure 3

A single PITX binding element exists within −61/−40 of the Fshb promoter. A, Sequences of the probes used in the gel shift analysis in B; − indicates no change in sequence from the WT probe. B, Gel shift assay with radiolabeled −61/−40 probe and nuclear extracts from CHO cells overexpressing PITX1. Complexes were competed with the indicated unlabeled probes (500-fold molar excess). Free probe is not pictured.