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. Author manuscript; available in PMC: 2009 Jan 8.
Published in final edited form as: Biochemistry. 2007 Dec 15;47(1):479–489. doi: 10.1021/bi7013882

Fig 3. Biological activity of PRL-Gluc and the relative ability of the intact and deleted PRLRs on HEK293 cells to bind and internalize PRL-Gluc.

Fig 3

Fig 3

Panel A compares diluted conditioned medium with unmodified human PRL. PRL-Gluc was biologically active, reaching the same maximum as unmodified PRL. OD 492 nm is absorbance in the MTS assay and is representative of relative cell number. A dilution of conditioned medium with bioactivity equivalent to 50ng/ml unmodified PRL was used for the studies in panel B. After a 3h incubation at 37°C, the cells were washed three times and cell-associated luminescence was determined. Non-specific binding/uptake (binding/uptake in the presence of excess unmodified PRL for each receptor type) was subtracted from each result. ** significantly different from their intact counterpart with p<0.01.