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. 1992 Mar;66(3):1795–1798. doi: 10.1128/jvi.66.3.1795-1798.1992

A chimera of EBNA1 and the estrogen receptor activates transcription but not replication.

T Middleton 1, B Sugden 1
PMCID: PMC240942  PMID: 1738208

Abstract

DNA replication from the plasmid origin of replication of Epstein-Barr virus requires one viral protein, EBNA1. This protein also acts as a transcriptional activator. Mutational analyses of EBNA1 have led to the conclusion that it supports transcription and DNA replication similarly. Such analyses have not probed the DNA-binding domain of EBNA1. To test whether domains of EBNA1 specifically required for either transcription or replication lie within its DNA-binding domain, we constructed a functional transcriptional activator by placing the EBNA1 DNA-binding domain in the context of the activation domains of the estrogen receptor. This hybrid protein did not support DNA replication, which indicates that the DNA-binding domain does not contain a replication-specific domain that can function along with heterologous transcriptional activating domains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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