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Journal of Virology logoLink to Journal of Virology
. 1991 Jun;65(6):3114–3121. doi: 10.1128/jvi.65.6.3114-3121.1991

Expression of a human cytomegalovirus receptor correlates with infectibility of cells.

D M Nowlin 1, N R Cooper 1, T Compton 1
PMCID: PMC240967  PMID: 1851872

Abstract

Previous studies have demonstrated that human cytomegalovirus (HCMV) specifically binds to a fibroblast membrane glycoprotein(s) with a molecular mass from 30 to 34 kDa. In this study, the distribution of the putative receptor proteins was analyzed in a variety of cell types, including cell types representative of those that are infected in vivo. Using a sensitive microbinding assay (to score virus attachment) and an indirect detection method (to score HCMV-binding proteins), we found that the 34- and 32-kDa HCMV binding proteins are ubiquitous molecules, broadly distributed among diverse cell types. In addition, the level of virus attachment was found to correlate with the abundance of the 34- and 32-kDa cellular proteins, while the ability of the virus to penetrate cells and initiate infection did not. The results support the hypothesis that the 34- and 32-kDa cellular proteins represent the HCMV (attachment) receptor. The data also support the notion that additional cellular components are required for virus entry and fusion.

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Selected References

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