Primary breast sarcoma: clinicopathologic series from the Mayo Clinic and review of the literature

Abstract

Primary sarcomas of the breast are extremely rare, with less than 0.1% of all malignant tumours of the breast. Mayo Clinic Surgical Pathology database was searched for all breast sarcoma from 1910 to 2000. Pathology reports and slides were reviewed and tumour types were determined. Metaplastic carcinomas and phyllodes tumours were excluded. There were 25 women ranging in age 24–81 years (mean 45 years). All but one patient presented with a palpable lump. Mastectomy was performed in 19 patients and lumpectomy in five patients. Histopathological diagnoses were fibrosarcoma (six), angiosarcoma (six), pleomorphic sarcoma (six), leiomyosarcoma (two), myxofibrosarcoma (three), hemangiopericytoma (one) and osteosarcoma (one). Tumour size ranged from 0.3 to 12 cm (mean 5.7). Low-grade lesions were observed in 10 cases and high-grade in 15. Overall, mean follow-up was 10.5 years. Local recurrence was observed in 11 patients and ranged from 2 to 36 months (mean 15 m), while distant metastasis was observed in 10 patients (40%) affecting lungs, bones, liver, spleen, and skin. Of the 25 patients, 12 have died of disease and six of other causes. Five-year overall (OS) and cause-specific survival (CSS) were 66 and 70%, respectively. OS and DFS at 5 years were 91% for tumours ⩽5 cm and 50% for tumours >5 cm. Tumour size was significantly associated with OS (risk ratio=1.3 per 1 cm increase; 95% CI, 1.02–1.7; P=0.036). There was no significant difference in OS or CSS between low- and high-grade lesions. In this series, tumour size was a more valuable prognostic factor than tumour grade.

Primary sarcomas of the breast are rare, malignant tumours arising from the mesenchymal tissue of the mammary gland (Oberman, 1965; Barnes and Pietruszka, 1977; Callery et al, 1985), with an approximate incidence of 17 new cases per million women (Moore and Kinne, 1996). At the Mayo Clinic, 27 881 malignant breast tumours were seen between 1940 and 1999 (C Adem, personal unpublished data) and 18 breast sarcomas were diagnosed accounting for 0.0006% of breast malignancies.

Breast sarcomas should be distinguished from metaplastic carcinomas (Adem et al, 2002). When facing a spindle cell neoplasm in an epithelial organ such as the breast one should be careful in rendering the diagnosis of sarcoma. In this setting, immunohistochemistry using the right antibodies is of major input. Berg et al defined stromal sarcomas of the breast in 1962 as a group of mesenchymal malignant tumours with fibrous, myxoid and adipose components, excluding malignant cystosarcoma phyllodes, lymphomas and angiosarcomas (Berg et al, 1962). However, series in the literature have included many different entities under the rubric of sarcomas such as cystosarcoma phyllodes, lymphosarcoma and carcinosarcoma (Botham et al, 1958; Donegan, 1967; Fawcett, 1967; Kennedy and Biggart, 1967; Rissanen and Holsti, 1968; Gogas et al, 1976; Ludgate et al, 1977; Khanna et al, 1981; Christensen et al, 1988; Terrier et al, 1989; Pitts et al, 1991; Ciatto et al, 1992; Luna Vega et al, 1992; McGregor et al, 1994; Moore and Kinne, 1996; McGowan et al, 2000). For this review, we choose to categorise primary breast sarcomas in histogenic terms, similar to other soft-tissue sarcomas, thus including angiosarcomas, and excluding malignant cystosarcomas phyllodes, as reported by others (see Table 1 ) (Berg et al, 1962; Oberman, 1965; Norris and Taylor, 1968; Barnes and Pietruszka, 1977; Callery et al, 1985; Stanley et al, 1988; Pollard et al, 1990; Johnstone et al, 1993; Smola et al, 1993; Gutman et al, 1994; North et al, 1998; Barrow et al, 1999).

Table 1. Major breast sarcomas comparable series in the English literature.

Author	N cases/period	Median age (years)	Median size (cm)	Diagnosis	Prognostic factors
Barnes and Pietruszka (1977)	10/31 years	51	6.3	5F, 1RMS, 1Le, 2OGS, 1 Li	Tumour contour, atypia, mitosis
Barrow et al (1999)	59/43 years	45	UK	32F, 17A, 1OGS, 7 NOS	Size, margins status, type
Berg et al (1962)	25/UK	48	6.0	Li and F	Positive margins
Callery et al (1985)	25/33 years	54	4.0	9F, 5M, 1HPC, 2Le, 2D, 3Desmoid, 1Li, 2 SS	UK
Gutman et al (1994)	60/51 years	48	6.5	17A, 16SS, 10F, 6M, 3O, 2Li, 2Le, 1R, 3U	Size, multifocal lesions, vascular, lymphatic, skin or chest wall invasion
Johnstone et al (1993)	10/12 years	28	UK	4A, 2M, 1R, 1Li, 1SS, 1Sc	UK
Norris and Taylor (1968)	32/UK	49	4.0	5 OGS, 1 Le/R, 3 Li, 1D, 22F	Size, contour, atypia, mitotic activity
North et al (1998)	25/31 years	55	6.0	10A, 5SS, 3F, 2Li, 2Le, 1M, 1OGS, 1 U	Type of surgery
Oberman (1965)	13/30 y	56	7.1	7f, 3R, 2D, 1MM	Size, type of surgery
Pollard et al (1990)	25/81 years	55.4	5.9	11M, 6Li, 4F, 1CC, 1NS, 1Le, 1ASP	Type of surgery
Smola et al (1993)	8/23 years	56	12.8	2CHS, 1M, 2Li, 2F, 1A	UK
Stanley et al (1988)	4/UK	61	UK	2M, 2A	UK
Zelek et al (2003)	83/37 years	47	6.5	58M, 8A, 7L, 2Sc, 2R, 2OGS, 2Le, 2O	Grade, size

A = angiosarcoma; SS = stromal sarcoma; F = fibrosarcoma; M = malignant fibrous histiocytoma; Li = liposarcoma; D = dermatofibrosarcoma protuberans; Sc = spindle cell sarcoma; Cs = carcinosarcoma; Le = leiomyosarcoma; R = rhabdomyosarcoma; U = unspecified; CC = clear cell sarcoma; ASP = alveolar soft part sarcoma; MM = malignant mesenchymoma; OGS = osteosarcoma; Others = O.

MATERIALS AND METHODS

All cases diagnosed pathologically at our institution from 1910 to 2000 as breast sarcomas and stromal sarcomas were retrieved from Mayo Clinic Surgical Pathology files.

The H&E-stained sections were examined in all cases to confirm the diagnosis. An average of seven (range, 1–28) H&E slides per case were available. Clinical charts and surgical notes were retrospectively reviewed and the following information was collected: age, gender, size of tumour, clinical presentation, duration of symptom, history of radiation, type of surgery, local recurrences and systemic metastases. Follow-up information was obtained from patient records and death certificates. Patients with other prior primary malignancy in the breast, radiation therapy and metastatic disease to the breast were excluded.

Patients with cystosarcoma phyllodes were excluded, as well as patients with metaplastic carcinoma. For this purpose, immunoperoxidase studies were performed using two primary antibodies, vimentin, to determine immunocompetence and wide spectrum screening keratin, to diagnose a metaplastic carcinoma as reported earlier (Adem et al, 2002). In regards of the fact that some cases were diagnosed at the beginning of the century, if immunoperoxidase study with vimentin was negative, another block representative of the tumour was chosen for further immunostaining. If vimentin staining was still negative, search for an internal control such as normal or carcinomatous component was done in each case.

Size, diagnosis, infiltrative or nodular pattern, presence of heterologous elements, grade according to Broders' scheme of grading used at the Mayo Clinic, mitotic index (in 10 high-power fields, using a Leitz microscope, field diameter 0.45 mm), and necrosis were assessed.

Overall survival (OS) and cause-specific survival (CSS) following diagnosis were estimated based on the Kaplan–Meier method, overall and separately for morphological features. Associations between morphologic features and survival were evaluated univariately based on fitting Cox proportional hazards models. All calculated P-values were two-sided and P-values less than 0.05 were considered statistically significant.

RESULTS

In all, 42 patients were retrieved between 1910 and 2000. Six were excluded after morphological review for the following reasons: cystosarcoma phyllodes (n=4), fibromatosis (n=1), benign haemangioma (n=1). Totally, 11 cases were also excluded after showing a positive stain with wide spectrum screening keratin, and being considered metaplastic carcinoma.

Clinical data

Overall, 25 remaining patients constituted the study group and are summarised in Table 2 . There were 25 women age range 24–81 (mean 45 years). In total, 24 cases presented with lump, two of them associated with pain. In one case, it presented as an incidental mammographic finding. Contralateral breast sarcoma had been diagnosed elsewhere 3 years earlier in one case, renal cell carcinoma 5 years later in one case, colon cancer 4 years earlier in one case, skin melanoma and uterine cancer in one case 16 and 27 years earlier, respectively. No history of prior radiation was found in any case, therefore excluding postradiation sarcoma. The duration of symptoms for 16 patients ranged between 1 month to 40 years (mean 3.2 years).

Table 2. Patients clinical and pathological characteristics in our series.

	Age (years)	Diagnosis	Duration	Surgery	Adjuvant therapy	Size	Gross	Margins	Grade	Local recurrence	Metastases	Last follow-up
Case 1	38	MXFS	15 m	R Mast	N	UK	C	N	2	3 y, S	N	DUK, 45 m
Case 2	38	F	UK	R Mast	RT	5	UK	UK	2	N	N	DOC, 5.5 y
Case 3	31	PS	2 m	Excision	N	UK	UK	I	4	1 y, R Mast	N	DOC, 1 y
Case 4	38	A	UK	Excision	N	UK	UK	I	2	5 times, 3 to 6 y, S/RT	L, 6 y	DOD, 84 m
Case 5	72	PS	UK	R Mast	N	3	I	I	4	N	N	Alive, 18 y
Case 6	49	F	1 m	Mast	UK	3	UK	I	3	N	N	DOC, 37 y
Case 7	43	A	1 m	Mast	N	8	C	I	1	N	B/L, 1 y, S/CT	DOD, 16 m
Case 8	48	MXFS	96 m	Mast	RT	5.5	I	I	3	4 m, No TTT	N	AWD, 6 m
Case 9	55	F	2 m	Mast	UK	UK	UK	N	2	Twice, 11 m and 17 m, S	N	DOD, 76 m
Case 10	67	Le	UK	Excision	CT	2	UK	UK	4	N	Li/B/Skin, at presentation, CT	DOD, 7 m
Case 11	39	A	2 m	Excision	N	8	I	I	2	20 m, S	Li/Jejunum, 9 y, None	DOD, 114 m
Case 12	32	PS	2 m	Excision	N	UK	UK	I	4	2 m, S	N	DOD, 23 m
Case 13	52	F	4 m	M R Mast	N	4.5	C	I	3	N	N	Alive, NED, 23.5 y
Case 14	27	A	11 m	S Mast	N	12	I	I	2	11 m, UK	L/Li/S, 22 m, UK	DOD, 32 m
Case 15	63	MXFS	30 y	S Mast	N	4	C	N	2	N	N	DOC, 21 y
Case 16	60	PS	12 m	R Mast	RT	10	C	N	4	N	B/Lu, 6 y, RT	DOD, 88 m
Case 17	55	Le	UK	Mast	N	4	UK	N	4	N	Multiple sites, 6 y, CT	DOD, 77 m
Case 18	33	A	12 m	R Mast	RT	10	C	I	2	10 m, UK	B, 10 m, RT	DOD, 13 m
Case 19	33	HPC	UK	UK	UK	UK	UK	N	4	N	L/Li/Pelvis, UK, RT	DOD, 41 m
Case 20	24	PS	12	S Mast	N	5	C	I	3	N	N	Alive, NED, 11 y
Case 21	32	A	11 m	S Mast	N	UK	UK	I	3	14 m, RT	B/L, 14 m, RT/CT	DOD, 26 m
Case 22	42	F	UK	S Mast	N	3	UK	I	3	8 m, S	N	DOC, 49 y
Case 23	54	F	1 m	M R Mast	N	5	UK	N	2	N	N	Alive, NED, 13 y
Case 24	81	PS	UK	M R Mast	N	0.3	UK	I	4	N	N	Alive, NED, 14 m
Case 25	54	OGS	UK	M R Mast	N	10	C	I	3	N	N	Alive, NED, 4 y

Abbreviations: MXFS = myxofibrosarcoma; F = fibrosarcoma; PS = pleomorphic sarcoma; AGS = angiosarcoma; Le = leiomyosarcoma; HPC = hemangiopericytoma; OGS = osteosarcoma; UK = unknown; R = radical; Mast = mastectomy; S = simple; M = modified; RT = radiotherapy; CT = chemotherapy; C = circumscribed; I = infiltrative; y = year; m = month; DOC = dead of other causes; DUK = dead of unknown cause; NED = no evidence of disease; DOD = dead of disease; Lu = lung; B = bone; Li = liver; S = spleen; N = nodular or pushing margins; I = infiltrative.

Surgical treatment was excision in five cases, mastectomy in 19 cases (modified, four; simple, five; radical, five; not specified, five), and unknown in one case. Adjuvant therapy was administered in five cases (radiation, four; chemotherapy, one).

The right breast was affected in 10 cases, while the left was affected in 15 cases.

Pathological data

Gross description was available in 12 cases. Eight tumours were described as well-circumscribed, four as infiltrative of which two were angiosarcoma. Tumour size was available on 18 patients, and the mean tumour size was 5.7 cm (range 0.3–12.0). Angiosarcomas tended to be larger in size with a mean of 10 cm (range, 8–12 cm).

After present review, histopathological diagnoses were fibrosarcoma (n=6), angiosarcoma (n=6), pleomorphic sarcoma (n=6), leiomyosarcoma (n=2), myxofibrosarcoma (n=3), hemangiopericytoma (n=1) and osteosarcoma (n=1). Tumours were graded as low grade (grade 1, one; grade 2, nine), and high grade (grade 3, seven; grade 4, eight). Necrosis was observed in four cases (three high-grade tumours). In all, 11 (range, 0–43) mitoses were found on average in 10 HPF. Heterologous component was seen in one case of osteosarcoma. Seven had pushing margins while 16 had infiltrative ones.

An in situ ductal carcinoma component was observed in one case. In this case of pleomorphic sarcoma, keratin staining was negative in neoplastic cells with adequate internal control (the in situ component as well as benign entrapped ducts).

There was no metastasis in the 15 cases where axillary node dissection was performed.

Follow-up and survival analysis (Figure 1)

Figure 1.

Overall survival following surgery, according to tumour size (⩽5 vs >5 cm). The numbers in parentheses indicate the number of patients still at risk at selected time points.

Overall mean and median follow-up were, respectively, 10.5 and 6.4 years (range, 7 months–41 years). Local recurrence was observed in 11 patients and ranged from 2 to 36 months (mean 15 months), while distant metastasis was observed in 10 patients, in order of frequency affecting the lungs (n=7), bones (n=6), liver (n=5), spleen (two) and skin (two). In one case, other sites were also kidney, pancreas, adrenal, omentum, epicardium and mediastinum. Of the 25 patients, 12 have died of disease and six of other causes. At the last follow-up, seven patients were still alive with a mean and median follow-up of 10.2 and 10.9 years, respectively.

Five-year overall (OS) and cause-specific survival (CSS) were 66 and 70%, respectively. Five-year OS and CSS were both 91% for tumours ⩽5 cm, and 50% for tumours >5 cm. Tumour size was significantly associated with OS (risk ratio=1.3 per 1 cm increase; 95% CI, 1.02–1.7; P=0.036). There was no significant difference between low- and high-grade lesions (OS were 60 and 70%, P=0.14, CSS were 70 and 70%, P=0.5, respectively) or tumours showing infiltrative compared to pushing margins (OS were 65 and 71%, P=0.47, CSS were 65 and 86%, P=0.94, respectively) in terms of OS or CSS.

Although there was no statistically significant association between tumour size and metastasis or recurrence, mean tumour size of patients with recurrence or metastasis was 7.7 cm, compared to 4.9 and 4.3 cm, respectively, for patients without recurrence or metastasis. Four out of five patients treated with simple excision had recurrence or metastasis.

By the most common histopathologic types, all but one patient with angiosarcoma (4/5), one patient with fibrosarcoma, and two patients with pleomorphic sarcoma, died of disease.

DISCUSSION

Primary breast sarcomas are extremely rare (Moore and Kinne, 1996). In our institution, they compose 0.0006% of breast malignancies. They constitute a specific clinicopathologic entity and, therefore should be differentiated from the two main entities in differential diagnosis, cystosarcoma phyllodes and metaplastic carcinoma. Specific morphological features (biphasic tumour, with leaf-like architecture and epithelial component) recognise the former, and extensive sampling of the tumour can help when a stromal overgrowth is present. The latter is recognised on H&E sections by the presence of a carcinomatous component, or based on a cytokeratin immunopositivity of the neoplastic spindle cells.

Reported series in the English literature had included all three entities as breast sarcomas, and include in their reports angiosarcomas, desmoid tumours, and lymphosarcomas (Botham et al, 1958; Berg et al, 1962; Oberman, 1965; Donegan, 1967; Fawcett, 1967; Kennedy and Biggart, 1967; Norris and Taylor, 1968; Rissanen and Holsti, 1968; Gogas et al, 1976; Barnes and Pietruszka, 1977; Ludgate et al, 1977; Khanna et al, 1981; Callery et al, 1985; Christensen et al, 1988; Stanley et al, 1988; Terrier et al, 1989; Pollard et al, 1990; Pitts et al, 1991; Ciatto et al, 1992; Luna Vega et al, 1992; Johnstone et al, 1993; Smola et al, 1993; Gutman et al, 1994; McGregor et al, 1994; Moore and Kinne, 1996; North et al, 1998; Barrow et al, 1999; McGowan et al, 2000). Therefore, reliable assessments of prognostic factors are difficult to make based on the published literature. Table 1 depicts comparable major series using soft-tissue tumours as basis for classification.

Tumour size seems to be the most frequently reliable prognostic factor in many of these series, as in breast carcinomas and soft-tissue sarcomas (Oberman, 1965; Norris and Taylor, 1968; Gutman et al, 1994; Barrow et al, 1999; Zelek et al, 2003) Other reported prognostic factors are the histopathological diagnosis (Barrow et al, 1999), the infiltrative features (Norris and Taylor, 1968; Barnes and Pietruszka, 1977), the histopathologic grading (Norris and Taylor, 1968; Barnes and Pietruszka, 1977; Gutman et al, 1994; Barrow et al, 1999; Zelek et al, 2003), presence of positive margins (Berg et al, 1962; Barrow et al, 1999), and extent of surgery for local recurrence (Pollard et al, 1990; North et al, 1998). Some authors found age to be of prognostic importance (Ludgate et al, 1977). Margins status is a major risk factor for recurrence as it occurs in any neoplastic entity, and some authors advised adjuvant radiotherapy for cases with positive margins (Callery et al, 1985; Smola et al, 1993), or less than 2 cm of clear margins (McGowan et al, 2000). Treatment is generally based on a wide local excision, without axillary dissection (Barrow et al, 1999). Breast sarcomas bear different histogenesis than breast carcinomas as shown by cytogenetic studies (Garcia-Palazzo et al, 1992), and biological behaviour (Berg et al, 1962).

We believe that breast sarcomas are comparable to soft-tissue sarcomas seen elsewhere. They present mainly as a lump and size is a prognostic marker with 5 cm serving as a valuable cut point. Tumour grade did not correlate with the outcome in our series but statistical power was limited and this finding could be related to the small size of the series. Lymphatic spread is uncommon as shown by the absence of axillary lymph node metastasis in our cases, and therefore axillary node dissection is not necessary. When lymph node metastasis is present, the diagnosis of a metaplastic carcinoma should be considered even in the presence of a pure spindle cell neoplasm.