Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1991 Jun;65(6):3388–3394. doi: 10.1128/jvi.65.6.3388-3394.1991

Improvement of avian leukosis virus (ALV)-based retrovirus vectors by using different cis-acting sequences from ALVs.

F L Cosset 1, C Legras 1, J L Thomas 1, R M Molina 1, Y Chebloune 1, C Faure 1, V M Nigon 1, G Verdier 1
PMCID: PMC241003  PMID: 1851887

Abstract

Production and expression of double-expression vectors which transduce both Neo(r) and lacZ genes and are based on the structure of avian leukosis virus were enhanced by using cis-acting sequences (long terminal repeats and noncoding sequences) from Rous-associated virus-1 and Rous-associated virus-2 rather than those of avian erythroblastosis virus previously used in our constructs. Polyclonal producer cells obtained after transfection of these vectors into the Isolde packaging cell line gave rise to titers as high as 3 x 10(5) lacZ CFU/ml, whereas it was possible to isolate clones of producer cells giving rise to titers of more than 10(6) resistance focus-forming units per ml.

Full text

PDF
3388

Images in this article

3392Image
on p.3392

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armentano D., Yu S. F., Kantoff P. W., von Ruden T., Anderson W. F., Gilboa E. Effect of internal viral sequences on the utility of retroviral vectors. J Virol. 1987 May;61(5):1647–1650. doi: 10.1128/jvi.61.5.1647-1650.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arrigo S., Beemon K. Regulation of Rous sarcoma virus RNA splicing and stability. Mol Cell Biol. 1988 Nov;8(11):4858–4867. doi: 10.1128/mcb.8.11.4858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Arrigo S., Yun M., Beemon K. cis-acting regulatory elements within gag genes of avian retroviruses. Mol Cell Biol. 1987 Jan;7(1):388–397. doi: 10.1128/mcb.7.1.388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Auffray C., Rougeon F. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem. 1980 Jun;107(2):303–314. doi: 10.1111/j.1432-1033.1980.tb06030.x. [DOI] [PubMed] [Google Scholar]
  5. Bandyopadhyay P. K., Watanabe S., Temin H. M. Recombination of transfected DNAs in vertebrate cells in culture. Proc Natl Acad Sci U S A. 1984 Jun;81(11):3476–3480. doi: 10.1073/pnas.81.11.3476. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Benchaibi M., Mallet F., Thoraval P., Savatier P., Xiao J. H., Verdier G., Samarut J., Nigon V. Avian retroviral vectors derived from avian defective leukemia virus: role of the translational context of the inserted gene on efficiency of the vectors. Virology. 1989 Mar;169(1):15–26. doi: 10.1016/0042-6822(89)90036-6. [DOI] [PubMed] [Google Scholar]
  7. Bender M. A., Palmer T. D., Gelinas R. E., Miller A. D. Evidence that the packaging signal of Moloney murine leukemia virus extends into the gag region. J Virol. 1987 May;61(5):1639–1646. doi: 10.1128/jvi.61.5.1639-1646.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Bieth E., Gabus C., Darlix J. L. A study of the dimer formation of Rous sarcoma virus RNA and of its effect on viral protein synthesis in vitro. Nucleic Acids Res. 1990 Jan 11;18(1):119–127. doi: 10.1093/nar/18.1.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Bizub D., Katz R. A., Skalka A. M. Nucleotide sequence of noncoding regions in Rous-associated virus-2: comparisons delineate conserved regions important in replication and oncogenesis. J Virol. 1984 Feb;49(2):557–565. doi: 10.1128/jvi.49.2.557-565.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Carlberg K., Beemon K. Proposed gag-encoded transcriptional activator is not necessary for Rous sarcoma virus replication or transformation. J Virol. 1988 Nov;62(11):4003–4008. doi: 10.1128/jvi.62.11.4003-4008.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Cepko C. L., Roberts B. E., Mulligan R. C. Construction and applications of a highly transmissible murine retrovirus shuttle vector. Cell. 1984 Jul;37(3):1053–1062. doi: 10.1016/0092-8674(84)90440-9. [DOI] [PubMed] [Google Scholar]
  12. Cosset F. L., Legras C., Chebloune Y., Savatier P., Thoraval P., Thomas J. L., Samarut J., Nigon V. M., Verdier G. A new avian leukosis virus-based packaging cell line that uses two separate transcomplementing helper genomes. J Virol. 1990 Mar;64(3):1070–1078. doi: 10.1128/jvi.64.3.1070-1078.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Cullen B. R., Raymond K., Ju G. Functional analysis of the transcription control region located within the avian retroviral long terminal repeat. Mol Cell Biol. 1985 Mar;5(3):438–447. doi: 10.1128/mcb.5.3.438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Cullen B. R., Raymond K., Ju G. Transcriptional activity of avian retroviral long terminal repeats directly correlates with enhancer activity. J Virol. 1985 Feb;53(2):515–521. doi: 10.1128/jvi.53.2.515-521.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Cullen B. R., Skalka A. M., Ju G. Endogenous avian retroviruses contain deficient promoter and leader sequences. Proc Natl Acad Sci U S A. 1983 May;80(10):2946–2950. doi: 10.1073/pnas.80.10.2946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Darlix J. L. Control of Rous sarcoma virus RNA translation and packaging by the 5' and 3' untranslated sequences. J Mol Biol. 1986 Jun 5;189(3):421–434. doi: 10.1016/0022-2836(86)90314-1. [DOI] [PubMed] [Google Scholar]
  17. Darlix J. L., Zuker M., Spahr P. F. Structure-function relationship of Rous sarcoma virus leader RNA. Nucleic Acids Res. 1982 Sep 11;10(17):5183–5196. doi: 10.1093/nar/10.17.5183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Dougherty J. P., Temin H. M. A promoterless retroviral vector indicates that there are sequences in U3 required for 3' RNA processing. Proc Natl Acad Sci U S A. 1987 Mar;84(5):1197–1201. doi: 10.1073/pnas.84.5.1197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ficht T. A., Chang L. J., Stoltzfus C. M. Avian sarcoma virus gag and env gene structural protein precursors contain a common amino-terminal sequence. Proc Natl Acad Sci U S A. 1984 Jan;81(2):362–366. doi: 10.1073/pnas.81.2.362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Galileo D. S., Gray G. E., Owens G. C., Majors J., Sanes J. R. Neurons and glia arise from a common progenitor in chicken optic tectum: demonstration with two retroviruses and cell type-specific antibodies. Proc Natl Acad Sci U S A. 1990 Jan;87(1):458–462. doi: 10.1073/pnas.87.1.458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Gandrillon O., Jurdic P., Benchaibi M., Xiao J. H., Ghysdael J., Samarut J. Expression of the v-erbA oncogene in chicken embryo fibroblasts stimulates their proliferation in vitro and enhances tumor growth in vivo. Cell. 1987 Jun 5;49(5):687–697. doi: 10.1016/0092-8674(87)90545-9. [DOI] [PubMed] [Google Scholar]
  22. Garver R. I., Jr, Chytil A., Karlsson S., Fells G. A., Brantly M. L., Courtney M., Kantoff P. W., Nienhuis A. W., Anderson W. F., Crystal R. G. Production of glycosylated physiologically "normal" human alpha 1-antitrypsin by mouse fibroblasts modified by insertion of a human alpha 1-antitrypsin cDNA using a retroviral vector. Proc Natl Acad Sci U S A. 1987 Feb;84(4):1050–1054. doi: 10.1073/pnas.84.4.1050. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Gowda S., Rao A. S., Kim Y. W., Guntaka R. V. Identification of sequences in the long terminal repeat of avian sarcoma virus required for efficient transcription. Virology. 1988 Jan;162(1):243–247. doi: 10.1016/0042-6822(88)90415-1. [DOI] [PubMed] [Google Scholar]
  24. Gray G. E., Glover J. C., Majors J., Sanes J. R. Radial arrangement of clonally related cells in the chicken optic tectum: lineage analysis with a recombinant retrovirus. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7356–7360. doi: 10.1073/pnas.85.19.7356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Hawley R. G., Covarrubias L., Hawley T., Mintz B. Handicapped retroviral vectors efficiently transduce foreign genes into hematopoietic stem cells. Proc Natl Acad Sci U S A. 1987 Apr;84(8):2406–2410. doi: 10.1073/pnas.84.8.2406. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Katz R. A., Cullen B. R., Malavarca R., Skalka A. M. Role of the avian retrovirus mRNA leader in expression: evidence for novel translational control. Mol Cell Biol. 1986 Feb;6(2):372–379. doi: 10.1128/mcb.6.2.372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Katz R. A., Terry R. W., Skalka A. M. A conserved cis-acting sequence in the 5' leader of avian sarcoma virus RNA is required for packaging. J Virol. 1986 Jul;59(1):163–167. doi: 10.1128/jvi.59.1.163-167.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. McIvor R. S., Johnson M. J., Miller A. D., Pitts S., Williams S. R., Valerio D., Martin D. W., Jr, Verma I. M. Human purine nucleoside phosphorylase and adenosine deaminase: gene transfer into cultured cells and murine hematopoietic stem cells by using recombinant amphotropic retroviruses. Mol Cell Biol. 1987 Feb;7(2):838–846. doi: 10.1128/mcb.7.2.838. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Miller A. D., Ong E. S., Rosenfeld M. G., Verma I. M., Evans R. M. Infectious and selectable retrovirus containing an inducible rat growth hormone minigene. Science. 1984 Sep 7;225(4666):993–998. doi: 10.1126/science.6089340. [DOI] [PubMed] [Google Scholar]
  30. Moscovici C., Moscovici M. G., Jimenez H., Lai M. M., Hayman M. J., Vogt P. K. Continuous tissue culture cell lines derived from chemically induced tumors of Japanese quail. Cell. 1977 May;11(1):95–103. doi: 10.1016/0092-8674(77)90320-8. [DOI] [PubMed] [Google Scholar]
  31. Overell R. W., Weisser K. E., Cosman D. Stably transmitted triple-promoter retroviral vectors and their use in transformation of primary mammalian cells. Mol Cell Biol. 1988 Apr;8(4):1803–1808. doi: 10.1128/mcb.8.4.1803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Pugatsch T., Stacey D. W. Identification of a sequence likely to be required for avian retroviral packaging. Virology. 1983 Jul 30;128(2):505–511. doi: 10.1016/0042-6822(83)90279-9. [DOI] [PubMed] [Google Scholar]
  33. Savatier P., Bagnis C., Thoraval P., Poncet D., Belakebi M., Mallet F., Legras C., Cosset F. L., Thomas J. L., Chebloune Y. Generation of a helper cell line for packaging avian leukosis virus-based vectors. J Virol. 1989 Feb;63(2):513–522. doi: 10.1128/jvi.63.2.513-522.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Schwartz D. E., Tizard R., Gilbert W. Nucleotide sequence of Rous sarcoma virus. Cell. 1983 Mar;32(3):853–869. doi: 10.1016/0092-8674(83)90071-5. [DOI] [PubMed] [Google Scholar]
  35. Simon J. A., Lis J. T. A germline transformation analysis reveals flexibility in the organization of heat shock consensus elements. Nucleic Acids Res. 1987 Apr 10;15(7):2971–2988. doi: 10.1093/nar/15.7.2971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Sorge J., Kuhl W., West C., Beutler E. Complete correction of the enzymatic defect of type I Gaucher disease fibroblasts by retroviral-mediated gene transfer. Proc Natl Acad Sci U S A. 1987 Feb;84(4):906–909. doi: 10.1073/pnas.84.4.906. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Sorge J., Ricci W., Hughes S. H. cis-Acting RNA packaging locus in the 115-nucleotide direct repeat of Rous sarcoma virus. J Virol. 1983 Dec;48(3):667–675. doi: 10.1128/jvi.48.3.667-675.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Stoker A. W., Hatier C., Bissell M. J. The embryonic environment strongly attenuates v-src oncogenesis in mesenchymal and epithelial tissues, but not in endothelia. J Cell Biol. 1990 Jul;111(1):217–228. doi: 10.1083/jcb.111.1.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Stoltzfus C. M., Fogarty S. J. Multiple regions in the Rous sarcoma virus src gene intron act in cis to affect the accumulation of unspliced RNA. J Virol. 1989 Apr;63(4):1669–1676. doi: 10.1128/jvi.63.4.1669-1676.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Thoraval P., Savatier P., Xiao J. H., Mallet F., Samarut J., Verdier G., Nigon V. Partial nucleotide sequence of the avian erythroblastosis virus (AEV ES4). Nucleic Acids Res. 1987 Nov 25;15(22):9612–9612. doi: 10.1093/nar/15.22.9612. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Yee J. K., Moores J. C., Jolly D. J., Wolff J. A., Respess J. G., Friedmann T. Gene expression from transcriptionally disabled retroviral vectors. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5197–5201. doi: 10.1073/pnas.84.15.5197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Yu S. F., von Rüden T., Kantoff P. W., Garber C., Seiberg M., Rüther U., Anderson W. F., Wagner E. F., Gilboa E. Self-inactivating retroviral vectors designed for transfer of whole genes into mammalian cells. Proc Natl Acad Sci U S A. 1986 May;83(10):3194–3198. doi: 10.1073/pnas.83.10.3194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. van der Putten H., Botteri F. M., Miller A. D., Rosenfeld M. G., Fan H., Evans R. M., Verma I. M. Efficient insertion of genes into the mouse germ line via retroviral vectors. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6148–6152. doi: 10.1073/pnas.82.18.6148. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES