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. 1992 May;66(5):2860–2864. doi: 10.1128/jvi.66.5.2860-2864.1992

The insertion site of the temperate phage HB-746 is located near the phage remnant in the pneumococcal host chromosome.

A Romero 1, R Lopez 1, P Garcia 1
PMCID: PMC241044  PMID: 1560530

Abstract

Combined Southern blot hybridization analyses of DNA digests of several clinical strains of Streptococcus pneumoniae have revealed the presence of a gene (hblR), or part of it, similar to the hbl7 gene coding for the cell wall lytic enzyme of the temperate HB-746 phage. The results confirmed that the genome of HB-746, which contains protein covalently linked to the 5' ends of its DNA, becomes integrated into the host strain 8R1 and showed that both the host and phage attachment sites, attB and attP, lie downstream of the 3' end of the structural region of the hblR and hbl7 genes, respectively. The data reported also highlight some evolutionary relationships between phage and bacteria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anilionis A., Riley M. Conservation and variation of nucleotide sequences within related bacterial genomes: Escherichia coli strains. J Bacteriol. 1980 Jul;143(1):355–365. doi: 10.1128/jb.143.1.355-365.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bernheimer H. P. Lysogenic pneumococci and their bacteriophages. J Bacteriol. 1979 May;138(2):618–624. doi: 10.1128/jb.138.2.618-624.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Craig N. L. The mechanism of conservative site-specific recombination. Annu Rev Genet. 1988;22:77–105. doi: 10.1146/annurev.ge.22.120188.000453. [DOI] [PubMed] [Google Scholar]
  4. Dowson C. G., Hutchison A., Brannigan J. A., George R. C., Hansman D., Liñares J., Tomasz A., Smith J. M., Spratt B. G. Horizontal transfer of penicillin-binding protein genes in penicillin-resistant clinical isolates of Streptococcus pneumoniae. Proc Natl Acad Sci U S A. 1989 Nov;86(22):8842–8846. doi: 10.1073/pnas.86.22.8842. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. García P., García J. L., García E., López R. Nucleotide sequence and expression of the pneumococcal autolysin gene from its own promoter in Escherichia coli. Gene. 1986;43(3):265–272. doi: 10.1016/0378-1119(86)90215-5. [DOI] [PubMed] [Google Scholar]
  6. García P., García J. L., García E., Sánchez-Puelles J. M., López R. Modular organization of the lytic enzymes of Streptococcus pneumoniae and its bacteriophages. Gene. 1990 Jan 31;86(1):81–88. doi: 10.1016/0378-1119(90)90116-9. [DOI] [PubMed] [Google Scholar]
  7. Gasc A. M., Kauc L., Barraillé P., Sicard M., Goodgal S. Gene localization, size, and physical map of the chromosome of Streptococcus pneumoniae. J Bacteriol. 1991 Nov;173(22):7361–7367. doi: 10.1128/jb.173.22.7361-7367.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Laible G., Spratt B. G., Hakenbeck R. Interspecies recombinational events during the evolution of altered PBP 2x genes in penicillin-resistant clinical isolates of Streptococcus pneumoniae. Mol Microbiol. 1991 Aug;5(8):1993–2002. doi: 10.1111/j.1365-2958.1991.tb00821.x. [DOI] [PubMed] [Google Scholar]
  9. Lee C. Y., Iandolo J. J. Integration of staphylococcal phage L54a occurs by site-specific recombination: structural analysis of the attachment sites. Proc Natl Acad Sci U S A. 1986 Aug;83(15):5474–5478. doi: 10.1073/pnas.83.15.5474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lee C. Y., Iandolo J. J. Structural analysis of staphylococcal bacteriophage phi 11 attachment sites. J Bacteriol. 1988 May;170(5):2409–2411. doi: 10.1128/jb.170.5.2409-2411.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Moynet D. J., Tiraby G. J. Inhibition of transformation in Streptococcus pneumoniae by lysogeny. J Bacteriol. 1980 Mar;141(3):1298–1304. doi: 10.1128/jb.141.3.1298-1304.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Muckerman C. C., Springhorn S. S., Greenberg B., Lacks S. A. Transformation of restriction endonuclease phenotype in Streptococcus pneumoniae. J Bacteriol. 1982 Oct;152(1):183–190. doi: 10.1128/jb.152.1.183-190.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Reanney D. C., Ackermann H. W. Comparative biology and evolution of bacteriophages. Adv Virus Res. 1982;27:205–280. doi: 10.1016/s0065-3527(08)60436-4. [DOI] [PubMed] [Google Scholar]
  14. Romero A., Lopez R., Garcia P. Characterization of the pneumococcal bacteriophage HB-3 amidase: cloning and expression in Escherichia coli. J Virol. 1990 Jan;64(1):137–142. doi: 10.1128/jvi.64.1.137-142.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Romero A., Lopez R., Garcia P. Sequence of the Streptococcus pneumoniae bacteriophage HB-3 amidase reveals high homology with the major host autolysin. J Bacteriol. 1990 Sep;172(9):5064–5070. doi: 10.1128/jb.172.9.5064-5070.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Romero A., Lopez R., Lurz R., Garcia P. Temperate bacteriophages of Streptococcus pneumoniae that contain protein covalently linked to the 5' ends of their DNA. J Virol. 1990 Oct;64(10):5149–5155. doi: 10.1128/jvi.64.10.5149-5155.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  18. Stroynowski I. T. Distribution of bacteriophage phi 3T homologous deoxyribonucleic acid sequences in Bacillus subtilis 168, related bacteriophages, and other Bacillus species. J Bacteriol. 1981 Oct;148(1):91–100. doi: 10.1128/jb.148.1.91-100.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Waldman A. S., Goodman S. D., Scocca J. J. Nucleotide sequences and properties of the sites involved in lysogenic insertion of the bacteriophage HP1c1 genome into the Haemophilus influenzae chromosome. J Bacteriol. 1987 Jan;169(1):238–246. doi: 10.1128/jb.169.1.238-246.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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