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. 1992 May;66(5):3125–3130. doi: 10.1128/jvi.66.5.3125-3130.1992

Mapping genetic determinants for human immunodeficiency virus type 1 resistance to soluble CD4.

W A O'Brien 1, I S Chen 1, D D Ho 1, E S Daar 1
PMCID: PMC241075  PMID: 1373203

Abstract

Neutralization of human immunodeficiency virus type 1 (HIV-1) infection with soluble CD4 (sCD4) can be achieved over a broad range of concentrations for different virus strains. Laboratory virus strains passaged in transformed T-cell lines are typically sensitive to sCD4 neutralization, whereas primary virus isolates require over 100-fold-higher sCD4 concentrations. Using recombinant viruses generated from a laboratory strain, HIV-1NL4-3, and a primary macrophagetropic strain, HIV-1JR-FL, we mapped a region of gp120 important for determining sensitivity to sCD4 neutralization. This same region has previously been defined as important for macrophage and transformed T-cell line tropism and includes the V3 neutralization domain but does not include regions of gp120 that have been shown to be most important for CD4 binding.

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Selected References

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