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. 1992 May;66(5):3206–3209. doi: 10.1128/jvi.66.5.3206-3209.1992

Human herpesvirus 7 is a constitutive inhabitant of adult human saliva.

L S Wyatt 1, N Frenkel 1
PMCID: PMC241088  PMID: 1348548

Abstract

We report the frequent isolation of human herpesvirus 7 from the saliva of healthy adults. Virus isolates recovered from different individuals exhibited minimal restriction enzyme polymorphism, which was mostly confined to heterogeneous (het) sequences in the genome. DNAs of isolates recovered from the same individual over a period of several months showed the same characteristic het fragments, indicating the stability of the het sequences upon virus replication and shedding in vivo. In contrast to the results of previous reports, human herpesvirus 6, the causative agent of roseola infantum, could not be isolated from the saliva specimens, raising questions regarding oral transmission of human herpesvirus 6 and human herpesvirus 7 to young children.

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Selected References

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  1. Asano Y., Nakashima T., Yoshikawa T., Suga S., Yazaki T. Severity of human herpesvirus-6 viremia and clinical findings in infants with exanthem subitum. J Pediatr. 1991 Jun;118(6):891–895. doi: 10.1016/s0022-3476(05)82200-0. [DOI] [PubMed] [Google Scholar]
  2. Asano Y., Yoshikawa T., Suga S., Yazaki T., Kondo K., Yamanishi K. Fatal fulminant hepatitis in an infant with human herpesvirus-6 infection. Lancet. 1990 Apr 7;335(8693):862–863. doi: 10.1016/0140-6736(90)90983-c. [DOI] [PubMed] [Google Scholar]
  3. Balachandran N., Amelse R. E., Zhou W. W., Chang C. K. Identification of proteins specific for human herpesvirus 6-infected human T cells. J Virol. 1989 Jun;63(6):2835–2840. doi: 10.1128/jvi.63.6.2835-2840.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carrigan D. R., Drobyski W. R., Russler S. K., Tapper M. A., Knox K. K., Ash R. C. Interstitial pneumonitis associated with human herpesvirus-6 infection after marrow transplantation. Lancet. 1991 Jul 20;338(8760):147–149. doi: 10.1016/0140-6736(91)90137-e. [DOI] [PubMed] [Google Scholar]
  5. Di Luca D., Katsafanas G., Schirmer E. C., Balachandran N., Frenkel N. The replication of viral and cellular DNA in human herpesvirus 6-infected cells. Virology. 1990 Mar;175(1):199–210. doi: 10.1016/0042-6822(90)90200-b. [DOI] [PubMed] [Google Scholar]
  6. Downing R. G., Sewankambo N., Serwadda D., Honess R., Crawford D., Jarrett R., Griffin B. E. Isolation of human lymphotropic herpesviruses from Uganda. Lancet. 1987 Aug 15;2(8555):390–390. doi: 10.1016/s0140-6736(87)92403-2. [DOI] [PubMed] [Google Scholar]
  7. Frenkel N., Schirmer E. C., Wyatt L. S., Katsafanas G., Roffman E., Danovich R. M., June C. H. Isolation of a new herpesvirus from human CD4+ T cells. Proc Natl Acad Sci U S A. 1990 Jan;87(2):748–752. doi: 10.1073/pnas.87.2.748. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gopal M. R., Thomson B. J., Fox J., Tedder R. S., Honess R. W. Detection by PCR of HHV-6 and EBV DNA in blood and oropharynx of healthy adults and HIV-seropositives. Lancet. 1990 Jun 30;335(8705):1598–1599. doi: 10.1016/0140-6736(90)91433-b. [DOI] [PubMed] [Google Scholar]
  9. Groopman J. E., Salahuddin S. Z., Sarngadharan M. G., Markham P. D., Gonda M., Sliski A., Gallo R. C. HTLV-III in saliva of people with AIDS-related complex and healthy homosexual men at risk for AIDS. Science. 1984 Oct 26;226(4673):447–449. doi: 10.1126/science.6093247. [DOI] [PubMed] [Google Scholar]
  10. Harnett G. B., Farr T. J., Pietroboni G. R., Bucens M. R. Frequent shedding of human herpesvirus 6 in saliva. J Med Virol. 1990 Feb;30(2):128–130. doi: 10.1002/jmv.1890300209. [DOI] [PubMed] [Google Scholar]
  11. Ho D. D., Byington R. E., Schooley R. T., Flynn T., Rota T. R., Hirsch M. S. Infrequency of isolation of HTLV-III virus from saliva in AIDS. N Engl J Med. 1985 Dec 19;313(25):1606–1606. doi: 10.1056/NEJM198512193132512. [DOI] [PubMed] [Google Scholar]
  12. Huang L. M., Lee C. Y., Lin K. H., Chuu W. M., Lee P. I., Chen R. L., Chen J. M., Lin D. T. Human herpesvirus-6 associated with fatal haemophagocytic syndrome. Lancet. 1990 Jul 7;336(8706):60–61. doi: 10.1016/0140-6736(90)91580-4. [DOI] [PubMed] [Google Scholar]
  13. Jarrett R. F., Clark D. A., Josephs S. F., Onions D. E. Detection of human herpesvirus-6 DNA in peripheral blood and saliva. J Med Virol. 1990 Sep;32(1):73–76. doi: 10.1002/jmv.1890320113. [DOI] [PubMed] [Google Scholar]
  14. Kido S., Kondo K., Kondo T., Morishima T., Takahashi M., Yamanishi K. Detection of human herpesvirus 6 DNA in throat swabs by polymerase chain reaction. J Med Virol. 1990 Nov;32(3):139–142. doi: 10.1002/jmv.1890320302. [DOI] [PubMed] [Google Scholar]
  15. Levy J. A., Ferro F., Greenspan D., Lennette E. T. Frequent isolation of HHV-6 from saliva and high seroprevalence of the virus in the population. Lancet. 1990 May 5;335(8697):1047–1050. doi: 10.1016/0140-6736(90)92628-u. [DOI] [PubMed] [Google Scholar]
  16. Levy J. A., Kaminsky L. S., Morrow W. J., Steimer K., Luciw P., Dina D., Hoxie J., Oshiro L. Infection by the retrovirus associated with the acquired immunodeficiency syndrome. Clinical, biological, and molecular features. Ann Intern Med. 1985 Nov;103(5):694–699. doi: 10.7326/0003-4819-103-5-694. [DOI] [PubMed] [Google Scholar]
  17. Lindquester G. J., Pellett P. E. Properties of the human herpesvirus 6 strain Z29 genome: G + C content, length, and presence of variable-length directly repeated terminal sequence elements. Virology. 1991 May;182(1):102–110. doi: 10.1016/0042-6822(91)90653-s. [DOI] [PubMed] [Google Scholar]
  18. Lopez C., Pellett P., Stewart J., Goldsmith C., Sanderlin K., Black J., Warfield D., Feorino P. Characteristics of human herpesvirus-6. J Infect Dis. 1988 Jun;157(6):1271–1273. doi: 10.1093/infdis/157.6.1271. [DOI] [PubMed] [Google Scholar]
  19. Martin M. E., Thomson B. J., Honess R. W., Craxton M. A., Gompels U. A., Liu M. Y., Littler E., Arrand J. R., Teo I., Jones M. D. The genome of human herpesvirus 6: maps of unit-length and concatemeric genomes for nine restriction endonucleases. J Gen Virol. 1991 Jan;72(Pt 1):157–168. doi: 10.1099/0022-1317-72-1-157. [DOI] [PubMed] [Google Scholar]
  20. Okuno T., Higashi K., Shiraki K., Yamanishi K., Takahashi M., Kokado Y., Ishibashi M., Takahara S., Sonoda T., Tanaka K. Human herpesvirus 6 infection in renal transplantation. Transplantation. 1990 Mar;49(3):519–522. doi: 10.1097/00007890-199003000-00009. [DOI] [PubMed] [Google Scholar]
  21. Okuno T., Takahashi K., Balachandra K., Shiraki K., Yamanishi K., Takahashi M., Baba K. Seroepidemiology of human herpesvirus 6 infection in normal children and adults. J Clin Microbiol. 1989 Apr;27(4):651–653. doi: 10.1128/jcm.27.4.651-653.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Pietroboni G. R., Harnett G. B., Bucens M. R., Honess R. W. Isolation of human herpesvirus 6 from saliva. Lancet. 1988 May 7;1(8593):1059–1059. doi: 10.1016/s0140-6736(88)91884-3. [DOI] [PubMed] [Google Scholar]
  23. Salahuddin S. Z., Ablashi D. V., Markham P. D., Josephs S. F., Sturzenegger S., Kaplan M., Halligan G., Biberfeld P., Wong-Staal F., Kramarsky B. Isolation of a new virus, HBLV, in patients with lymphoproliferative disorders. Science. 1986 Oct 31;234(4776):596–601. doi: 10.1126/science.2876520. [DOI] [PubMed] [Google Scholar]
  24. Salahuddin S. Z., Markham P. D., Popovic M., Sarngadharan M. G., Orndorff S., Fladagar A., Patel A., Gold J., Gallo R. C. Isolation of infectious human T-cell leukemia/lymphotropic virus type III (HTLV-III) from patients with acquired immunodeficiency syndrome (AIDS) or AIDS-related complex (ARC) and from healthy carriers: a study of risk groups and tissue sources. Proc Natl Acad Sci U S A. 1985 Aug;82(16):5530–5534. doi: 10.1073/pnas.82.16.5530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Saxinger C., Polesky H., Eby N., Grufferman S., Murphy R., Tegtmeir G., Parekh V., Memon S., Hung C. Antibody reactivity with HBLV (HHV-6) in U.S. populations. J Virol Methods. 1988 Sep;21(1-4):199–208. doi: 10.1016/0166-0934(88)90066-3. [DOI] [PubMed] [Google Scholar]
  26. Schirmer E. C., Wyatt L. S., Yamanishi K., Rodriguez W. J., Frenkel N. Differentiation between two distinct classes of viruses now classified as human herpesvirus 6. Proc Natl Acad Sci U S A. 1991 Jul 1;88(13):5922–5926. doi: 10.1073/pnas.88.13.5922. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Ueda K., Kusuhara K., Hirose M., Okada K., Miyazaki C., Tokugawa K., Nakayama M., Yamanishi K. Exanthem subitum and antibody to human herpesvirus-6. J Infect Dis. 1989 Apr;159(4):750–752. doi: 10.1093/infdis/159.4.750. [DOI] [PubMed] [Google Scholar]
  28. Wyatt L. S., Balachandran N., Frenkel N. Variations in the replication and antigenic properties of human herpesvirus 6 strains. J Infect Dis. 1990 Oct;162(4):852–857. doi: 10.1093/infdis/162.4.852. [DOI] [PubMed] [Google Scholar]
  29. Wyatt L. S., Rodriguez W. J., Balachandran N., Frenkel N. Human herpesvirus 7: antigenic properties and prevalence in children and adults. J Virol. 1991 Nov;65(11):6260–6265. doi: 10.1128/jvi.65.11.6260-6265.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Yamanishi K., Okuno T., Shiraki K., Takahashi M., Kondo T., Asano Y., Kurata T. Identification of human herpesvirus-6 as a causal agent for exanthem subitum. Lancet. 1988 May 14;1(8594):1065–1067. doi: 10.1016/s0140-6736(88)91893-4. [DOI] [PubMed] [Google Scholar]
  31. Yoshikawa T., Suga S., Asano Y., Nakashima T., Yazaki T., Sobue R., Hirano M., Fukuda M., Kojima S., Matsuyama T. Human herpesvirus-6 infection in bone marrow transplantation. Blood. 1991 Sep 1;78(5):1381–1384. [PubMed] [Google Scholar]

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