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. 1992 Jul;66(7):4085–4097. doi: 10.1128/jvi.66.7.4085-4097.1992

The surface envelope protein gene region of equine infectious anemia virus is not an important determinant of tropism in vitro.

S T Perry 1, M T Flaherty 1, M J Kelley 1, D L Clabough 1, S R Tronick 1, L Coggins 1, L Whetter 1, C R Lengel 1, F Fuller 1
PMCID: PMC241211  PMID: 1318398

Abstract

Virulent, wild-type equine infectious anemia virus (EIAV) is restricted in one or more early steps in replication in equine skin fibroblast cells compared with cell culture-adapted virus, which is fully competent for replication in this cell type. We compared the sequences of wild-type EIAV and a full-length infectious proviral clone of the cell culture-adapted EIAV and found that the genomes were relatively well conserved with the exception of the envelope gene region, which showed extensive sequence differences. We therefore constructed several wild-type and cell culture-adapted virus chimeras to examine the role of the envelope gene in replication in different cell types in vitro. Unlike wild-type virus, which is restricted by an early event(s) for replication in equine dermis cells, the wild-type outer envelope gene chimeras are replication competent in this cell type. We conclude that even though there are extensive sequence differences between wild-type and cell culture-adapted viruses in the surface envelope gene region, this domain is not a determinant of the differing in vitro cell tropisms.

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Selected References

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