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. 1992 Aug;66(8):4893–4900. doi: 10.1128/jvi.66.8.4893-4900.1992

Partial reverse transcripts in virions from human immunodeficiency and murine leukemia viruses.

D Trono 1
PMCID: PMC241327  PMID: 1378513

Abstract

Reverse transcription of the retroviral genome is thought to start after virions enter target cells. Purified preparations of human immunodeficiency virus were found to contain virus-specific DNA, detectable by polymerase chain reaction amplification. This DNA resulted from reverse transcription in newly assembled virus particles and not from contamination by cellular DNA, because virions contained a striking excess of early versus late transcripts and because the accumulation of these products was sensitive to 3'-azido-3'-deoxythymidine (zidovudine) treatment of producer cells. A similar observation was made with murine amphotropic retrovirus particles. It is therefore likely that all retroviruses contain partial reverse transcripts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D. RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature. 1970 Jun 27;226(5252):1209–1211. doi: 10.1038/2261209a0. [DOI] [PubMed] [Google Scholar]
  2. Besansky N. J., Butera S. T., Sinha S., Folks T. M. Unintegrated human immunodeficiency virus type 1 DNA in chronically infected cell lines is not correlated with surface CD4 expression. J Virol. 1991 May;65(5):2695–2698. doi: 10.1128/jvi.65.5.2695-2698.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Biswal N., McCain B., Maccain B., Benyeshh-Melnick M. The DNA of murine sarcoma-leukemia virus. Virology. 1971 Sep;45(3):697–706. doi: 10.1016/0042-6822(71)90183-8. [DOI] [PubMed] [Google Scholar]
  4. Collett M. S., Faras A. J. Evidence for circularization of the avian oncornavirus RNA genome during proviral DNA synthesis from studies of reverse transcription in vitro. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1329–1332. doi: 10.1073/pnas.73.4.1329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Crawford S., Goff S. P. A deletion mutation in the 5' part of the pol gene of Moloney murine leukemia virus blocks proteolytic processing of the gag and pol polyproteins. J Virol. 1985 Mar;53(3):899–907. doi: 10.1128/jvi.53.3.899-907.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Daley G. Q., Van Etten R. A., Baltimore D. Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome. Science. 1990 Feb 16;247(4944):824–830. doi: 10.1126/science.2406902. [DOI] [PubMed] [Google Scholar]
  7. Feinberg M. B., Baltimore D., Frankel A. D. The role of Tat in the human immunodeficiency virus life cycle indicates a primary effect on transcriptional elongation. Proc Natl Acad Sci U S A. 1991 May 1;88(9):4045–4049. doi: 10.1073/pnas.88.9.4045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Folks T. M., Clouse K. A., Justement J., Rabson A., Duh E., Kehrl J. H., Fauci A. S. Tumor necrosis factor alpha induces expression of human immunodeficiency virus in a chronically infected T-cell clone. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2365–2368. doi: 10.1073/pnas.86.7.2365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Junghans R. P., Duesberg P. H., Knight C. A. In vitro synthesis of full-length DNA transcripts of Rous sarcoma virus RNA by viral DNA polymerase. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4895–4899. doi: 10.1073/pnas.72.12.4895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kappes J. C., Conway J. A., Lee S. W., Shaw G. M., Hahn B. H. Human immunodeficiency virus type 2 vpx protein augments viral infectivity. Virology. 1991 Sep;184(1):197–209. doi: 10.1016/0042-6822(91)90836-z. [DOI] [PubMed] [Google Scholar]
  11. Katoh I., Yoshinaka Y., Rein A., Shibuya M., Odaka T., Oroszlan S. Murine leukemia virus maturation: protease region required for conversion from "immature" to "mature" core form and for virus infectivity. Virology. 1985 Sep;145(2):280–292. doi: 10.1016/0042-6822(85)90161-8. [DOI] [PubMed] [Google Scholar]
  12. Kim S. Y., Byrn R., Groopman J., Baltimore D. Temporal aspects of DNA and RNA synthesis during human immunodeficiency virus infection: evidence for differential gene expression. J Virol. 1989 Sep;63(9):3708–3713. doi: 10.1128/jvi.63.9.3708-3713.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Levinson W. E., Varmus H. E., Garapin A. C., Bishop J. M. DNA of Rous sarcoma virus: its nature and significance. Science. 1972 Jan 7;175(4017):76–78. doi: 10.1126/science.175.4017.76. [DOI] [PubMed] [Google Scholar]
  14. Levinson W., Bishop J. M., Quintrell N., Jackson J. Presence of DNA in Rous sarcoma virus. Nature. 1970 Sep 5;227(5262):1023–1025. doi: 10.1038/2271023a0. [DOI] [PubMed] [Google Scholar]
  15. Marcon L., Michaels F., Hattori N., Fargnoli K., Gallo R. C., Franchini G. Dispensable role of the human immunodeficiency virus type 2 Vpx protein in viral replication. J Virol. 1991 Jul;65(7):3938–3942. doi: 10.1128/jvi.65.7.3938-3942.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mason W. S., Taylor J. M., Hull R. Retroid virus genome replication. Adv Virus Res. 1987;32:35–96. doi: 10.1016/s0065-3527(08)60474-1. [DOI] [PubMed] [Google Scholar]
  17. Moore J. P., McKeating J. A., Norton W. A., Sattentau Q. J. Direct measurement of soluble CD4 binding to human immunodeficiency virus type 1 virions: gp120 dissociation and its implications for virus-cell binding and fusion reactions and their neutralization by soluble CD4. J Virol. 1991 Mar;65(3):1133–1140. doi: 10.1128/jvi.65.3.1133-1140.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Moore J. P., McKeating J. A., Weiss R. A., Sattentau Q. J. Dissociation of gp120 from HIV-1 virions induced by soluble CD4. Science. 1990 Nov 23;250(4984):1139–1142. doi: 10.1126/science.2251501. [DOI] [PubMed] [Google Scholar]
  19. Peng C., Ho B. K., Chang T. W., Chang N. T. Role of human immunodeficiency virus type 1-specific protease in core protein maturation and viral infectivity. J Virol. 1989 Jun;63(6):2550–2556. doi: 10.1128/jvi.63.6.2550-2556.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rokutanda M., Rokutanda H., Green M., Fujinaga K., Ray R. K., Gurgo C. Formation of viral RNA-DNA hybrid molecules by the DNA polymerase of sarcoma-leukaemia viruses. Nature. 1970 Sep 5;227(5262):1026–1028. doi: 10.1038/2271026a0. [DOI] [PubMed] [Google Scholar]
  21. Rothenberg E., Baltimore D. Increased length of DNA made by virions of murine leukemia virus at limiting magnesium ion concentration. J Virol. 1977 Jan;21(1):168–178. doi: 10.1128/jvi.21.1.168-178.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rothenberg E., Baltimore D. Synthesis of long, representative DNA copies of the murine RNA tumor virus genome. J Virol. 1975 Jan;17(1):168–174. doi: 10.1128/jvi.17.1.168-174.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rothenberg E., Smotkin D., Baltimore D., Weinberg R. A. In vitro synthesis of infectious DNA of murine leukaemia virus. Nature. 1977 Sep 8;269(5624):122–126. doi: 10.1038/269122a0. [DOI] [PubMed] [Google Scholar]
  24. Ríman J., Beaudreau G. S. Viral DNA-dependent DNA polymerase and the properties of thymidine labelled material in virions of an oncogenic RNA virus. Nature. 1970 Oct 31;228(5270):427–430. doi: 10.1038/228427a0. [DOI] [PubMed] [Google Scholar]
  25. Shaw G. M., Hahn B. H., Arya S. K., Groopman J. E., Gallo R. C., Wong-Staal F. Molecular characterization of human T-cell leukemia (lymphotropic) virus type III in the acquired immune deficiency syndrome. Science. 1984 Dec 7;226(4679):1165–1171. doi: 10.1126/science.6095449. [DOI] [PubMed] [Google Scholar]
  26. Shinnick T. M., Lerner R. A., Sutcliffe J. G. Nucleotide sequence of Moloney murine leukaemia virus. Nature. 1981 Oct 15;293(5833):543–548. doi: 10.1038/293543a0. [DOI] [PubMed] [Google Scholar]
  27. Stevenson M., Haggerty S., Lamonica C. A., Meier C. M., Welch S. K., Wasiak A. J. Integration is not necessary for expression of human immunodeficiency virus type 1 protein products. J Virol. 1990 May;64(5):2421–2425. doi: 10.1128/jvi.64.5.2421-2425.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Stevenson M., Stanwick T. L., Dempsey M. P., Lamonica C. A. HIV-1 replication is controlled at the level of T cell activation and proviral integration. EMBO J. 1990 May;9(5):1551–1560. doi: 10.1002/j.1460-2075.1990.tb08274.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Temin H. M., Mizutani S. RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature. 1970 Jun 27;226(5252):1211–1213. doi: 10.1038/2261211a0. [DOI] [PubMed] [Google Scholar]
  30. Trono D., Feinberg M. B., Baltimore D. HIV-1 Gag mutants can dominantly interfere with the replication of the wild-type virus. Cell. 1989 Oct 6;59(1):113–120. doi: 10.1016/0092-8674(89)90874-x. [DOI] [PubMed] [Google Scholar]
  31. Varmus H. E., Padgett T., Heasley S., Simon G., Bishop J. M. Cellular functions are required for the synthesis and integration of avian sarcoma virus-specific DNA. Cell. 1977 Jun;11(2):307–319. doi: 10.1016/0092-8674(77)90047-2. [DOI] [PubMed] [Google Scholar]
  32. Zack J. A., Arrigo S. J., Weitsman S. R., Go A. S., Haislip A., Chen I. S. HIV-1 entry into quiescent primary lymphocytes: molecular analysis reveals a labile, latent viral structure. Cell. 1990 Apr 20;61(2):213–222. doi: 10.1016/0092-8674(90)90802-l. [DOI] [PubMed] [Google Scholar]

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