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. 1991 Jul;65(7):3882–3886. doi: 10.1128/jvi.65.7.3882-3886.1991

Identification of a strong enhancer element upstream from the pregenomic RNA start site of the duck hepatitis B virus genome.

B Crescenzo-Chaigne 1, J Pillot 1, A Lilienbaum 1, M Levrero 1, E Elfassi 1
PMCID: PMC241420  PMID: 2041096

Abstract

The genome of the duck hepatitis B virus (DHBV) contains an enhancer element. This sequence, of 192 bp, is located in the 3'-terminal coding region of the DNA polymerase gene (nucleotides 2159 to 2351), upstream from the pregenomic RNA start site. This enhancer potentiates a marked increased activity from the heterologous thymidine kinase promoter in an orientation-independent manner and at a proximal, as well as a distal, location. The DHBV enhancer activates transcription in a relatively cell-type-independent manner. Sequence homologies with the nuclear factor EF-C binding site are located in the DHBV enhancer. By using the HepG2 nuclear extracts and the DHBV enhancer as probes, a complex was observed in mobility shift assays.

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Selected References

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