Abstract
A Bacillus subtilis strain, DSM704, was characterized by genetic means, and its production of a human intestinal sucrase inhibitor, 1-deoxynojirimycin, was described. Synthesis of this compound is detected concomitant with the detection of heat-resistant spores. The amount of 1-deoxynojirimycin produced is highly dependent on the carbon source, with growth on substrates metabolized via glycolysis giving the greatest amount of production (up to 1 mg/ml). 1-Deoxynojirimycin appears to be nonmetabolizable by the producing strain in that it cannot serve as a sole carbon or nitrogen source.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Boylan R. J., Mendelson N. H., Brooks D., Young F. E. Regulation of the bacterial cell wall: analysis of a mutant of Bacillus subtilis defective in biosynthesis of teichoic acid. J Bacteriol. 1972 Apr;110(1):281–290. doi: 10.1128/jb.110.1.281-290.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chattopadhyay S. P., Banerjee A. K. Production of valine by a Bacillus sp. Z Allg Mikrobiol. 1978;18(4):243–254. doi: 10.1002/jobm.3630180403. [DOI] [PubMed] [Google Scholar]
- Chilton M. D., McCarthy B. J. Genetic and base sequence homologies in bacillus. Genetics. 1969 Jul;62(3):697–710. [PMC free article] [PubMed] [Google Scholar]
- Hanlon G. W., Hodges N. A. Bacitracin and protease production in relation to sporulation during exponential growth of Bacillus licheniformis on poorly utilized carbon and nitrogen sources. J Bacteriol. 1981 Aug;147(2):427–431. doi: 10.1128/jb.147.2.427-431.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hanson R. S., Peterson J. A., Yousten A. A. Unique biochemical events in bacterial sporulation. Annu Rev Microbiol. 1970;24:53–90. doi: 10.1146/annurev.mi.24.100170.000413. [DOI] [PubMed] [Google Scholar]
- Hemphill H. E., Whiteley H. R. Bacteriophages of Bacillus subtilis. Bacteriol Rev. 1975 Sep;39(3):257–315. doi: 10.1128/br.39.3.257-315.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ingle M. B., Erickson R. J. Bacterial alpha-amylases. Adv Appl Microbiol. 1978;24:257–278. [PubMed] [Google Scholar]
- Ohné M., Rutberg B. Repression of sporulation in Bacillus subtilis by L-malate. J Bacteriol. 1976 Feb;125(2):453–460. doi: 10.1128/jb.125.2.453-460.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schaeffer P. Sporulation and the production of antibiotics, exoenzymes, and exotonins. Bacteriol Rev. 1969 Mar;33(1):48–71. doi: 10.1128/br.33.1.48-71.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schmidt D. D., Frommer W., Müller L., Truscheit E. Glucosidase-Inhibitoren aus Bazillen. Naturwissenschaften. 1979 Nov;66(11):584–585. doi: 10.1007/BF00368825. [DOI] [PubMed] [Google Scholar]
- Wilson G. A., Young F. E. Intergenotic transformation of the Bacillus subtilis genospecies. J Bacteriol. 1972 Sep;111(3):705–716. doi: 10.1128/jb.111.3.705-716.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- YOUNG F. E., SPIZIZEN J. Physiological and genetic factors affecting transformation of Bacillus subtilis. J Bacteriol. 1961 May;81:823–829. doi: 10.1128/jb.81.5.823-829.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yasbin R. E., Ganesan A. T., Young F. E. Bacteriophage interference in Bacillus subtilis 168. J Virol. 1974 Apr;13(4):916–921. doi: 10.1128/jvi.13.4.916-921.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yasbin R. E., Maino V. C., Young F. E. Bacteriophage resistance in Bacillus subtilis 168, W23, and interstrain transformants. J Bacteriol. 1976 Mar;125(3):1120–1126. doi: 10.1128/jb.125.3.1120-1126.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]