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. 1984 Aug;48(2):367–370. doi: 10.1128/aem.48.2.367-370.1984

Isolation of Aeromonas spp. from an unchlorinated domestic water supply.

V Burke, J Robinson, M Gracey, D Peterson, N Meyer, V Haley
PMCID: PMC241519  PMID: 6486783

Abstract

The recovery of Aeromonas spp. from the unchlorinated water supply for a Western Australian city of 21,000 people was monitored at several sampling points during a period of 1 year. Membrane filtration techniques were used to count colonies of Aeromonas spp., coliforms, and Escherichia coli in water sampled before entry to service reservoirs, during storage in service reservoirs, and in distribution systems. Aeromonas spp. were identified by subculture on blood agar with ampicillin, oxidase tests, and the use of Kaper medium and then were tested for production of enterotoxins and hemolysins. During the same period, two-thirds of all fecal specimens sent for microbiological examination were cultured on ampicillin-blood agar for Aeromonas spp. Recovery of Aeromonas spp. from water supplies at distribution points correlated with fecal isolations and continued during autumn and winter. Coliforms and E. coli were found most commonly in late summer to autumn. This pattern differs from the summer peak of Aeromonas isolations both from water and from patients with Aeromonas spp.-associated gastroenteritis in Perth, Western Australia, a city with a chlorinated domestic water supply. Of the Aeromonas strains from water, 61% were enterotoxigenic, and 64% produced hemolysins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burke V., Robinson J., Atkinson H. M., Gracey M. Biochemical characteristics of enterotoxigenic Aeromonas spp. J Clin Microbiol. 1982 Jan;15(1):48–52. doi: 10.1128/jcm.15.1.48-52.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burke V., Robinson J., Berry R. J., Gracey M. Detection of enterotoxins of Aeromonas hydrophila by a suckling-mouse test. J Med Microbiol. 1981 Nov;14(4):401–408. doi: 10.1099/00222615-14-4-401. [DOI] [PubMed] [Google Scholar]
  3. Burke V., Robinson J., Gracey M., Peterson D., Partridge K. Isolation of Aeromonas hydrophila from a metropolitan water supply: seasonal correlation with clinical isolates. Appl Environ Microbiol. 1984 Aug;48(2):361–366. doi: 10.1128/aem.48.2.361-366.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CARY S. G., BLAIR E. B. NEW TRANSPORT MEDIUM FOR SHIPMENT OF CLINICAL SPECIMENS. I. FECAL SPECIMENS. J Bacteriol. 1964 Jul;88:96–98. doi: 10.1128/jb.88.1.96-98.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cavari B. Z., Allen D. A., Colwell R. R. Effect of temperature on growth and activity of Aeromonas spp. and mixed bacterial populations in the Anacostia River. Appl Environ Microbiol. 1981 Apr;41(4):1052–1054. doi: 10.1128/aem.41.4.1052-1054.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clark J. A., Burger C. A., Sabatinos L. E. Characterization of indicator bacteria in municipal raw water, drinking water, and new main water samples. Can J Microbiol. 1982 Sep;28(9):1002–1013. doi: 10.1139/m82-150. [DOI] [PubMed] [Google Scholar]
  7. Davis W. A., 2nd, Kane J. G., Garagusi V. F. Human aeromonas infections: a review of the literature and a case report of endocarditis. Medicine (Baltimore) 1978 May;57(3):267–277. [PubMed] [Google Scholar]
  8. Fulghum D. D., Linton W. R., Taplin D. Fatal Aeromonas hydrophila infection of the skin. South Med J. 1978 Jun;71(6):739–741. doi: 10.1097/00007611-197806000-00037. [DOI] [PubMed] [Google Scholar]
  9. Gracey M., Burke V., Robinson J. Aeromonas-associated gastroenteritis. Lancet. 1982 Dec 11;2(8311):1304–1306. doi: 10.1016/s0140-6736(82)91510-0. [DOI] [PubMed] [Google Scholar]
  10. Hanson P. G., Standridge J., Jarrett F., Maki D. G. Freshwater wound infection due to Aeromonas hydrophila. JAMA. 1977 Sep 5;238(10):1053–1054. [PubMed] [Google Scholar]
  11. Hazen T. C., Fliermans C. B., Hirsch R. P., Esch G. W. Prevalence and distribution of Aeromonas hydrophila in the United States. Appl Environ Microbiol. 1978 Nov;36(5):731–738. doi: 10.1128/aem.36.5.731-738.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Joseph S. W., Daily O. P., Hunt W. S., Seidler R. J., Allen D. A., Colwell R. R. Aeromonas primary wound infection of a diver in polluted waters. J Clin Microbiol. 1979 Jul;10(1):46–49. doi: 10.1128/jcm.10.1.46-49.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kaper J. B., Lockman H., Colwell R. R., Joseph S. W. Aeromonas hydrophila: ecology and toxigenicity of isolates from an estuary. J Appl Bacteriol. 1981 Apr;50(2):359–377. doi: 10.1111/j.1365-2672.1981.tb00900.x. [DOI] [PubMed] [Google Scholar]
  14. Kaper J., Seidler R. J., Lockman H., Colwell R. R. Medium for the presumptive identification of Aeromonas hydrophila and Enterobacteriaceae. Appl Environ Microbiol. 1979 Nov;38(5):1023–1026. doi: 10.1128/aem.38.5.1023-1026.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SLANETZ L. W., BARTLEY C. H. Numbers of enterococci in water, sewage, and feces determined by the membrane filter technique with an improved medium. J Bacteriol. 1957 Nov;74(5):591–595. doi: 10.1128/jb.74.5.591-595.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schubert R. H. Das Vorkommen der Aeromonaden in oberirdischen Gewässern. Arch Hyg Bakteriol. 1967 Mar;150(7):688–708. [PubMed] [Google Scholar]
  17. Sen R., Jacobs B. Pathogenic intestinal organisms in the unfiltered water supply of Calcutta and the effect of chlorination. Indian J Med Res. 1969 Jul;57(7):1220–1227. [PubMed] [Google Scholar]
  18. Trust T. J., Chipman D. C. Clinical involvement of Aeromonas hydrophila. Can Med Assoc J. 1979 Apr 21;120(8):942–946. [PMC free article] [PubMed] [Google Scholar]
  19. Wolff R. L., Wiseman S. L., Kitchens C. S. Aeromonas hydrophila bacteremia in ambulatory immunocompromised hosts. Am J Med. 1980 Feb;68(2):238–242. doi: 10.1016/0002-9343(80)90360-5. [DOI] [PubMed] [Google Scholar]

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