Abstract
Field-collected larvae of the tussock moth, Lymantria ninayi, a major pest of exotic pines in Papua New Guinea, were found to contain a small RNA-containing virus with a diameter of 29 nm and a buoyant density of 1.32 g/ml. The RNA was single stranded, had a molecular weight of 2.8 × 106, and was polyadenylated. Virion RNA stimulated an in vitro translation system, and high-molecular-weight proteins were produced. Purified virions contained four structural proteins with molecular weights of 43,000, 38,000, 33,000, and 32,000. The virus reacted positively with antisera raised against a strain of Drosophila C virus. The properties of this virus indicate that it should be placed in the family Picornaviridae.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Moore N. F., Kearns A., Pullin J. S. Characterization of cricket paralysis virus-induced polypeptides in Drosophila cells. J Virol. 1980 Jan;33(1):1–9. doi: 10.1128/jvi.33.1.1-9.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moore N. F., Tinsley T. W. The small rna-viruses of insects. Arch Virol. 1982;72(4):229–245. doi: 10.1007/BF01315220. [DOI] [PubMed] [Google Scholar]
- Morrissey J. H. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem. 1981 Nov 1;117(2):307–310. doi: 10.1016/0003-2697(81)90783-1. [DOI] [PubMed] [Google Scholar]
- Reavy B., Moore N. F. In vitro translation of cricket paralysis virus RNA. Arch Virol. 1981;67(2):175–180. doi: 10.1007/BF01318602. [DOI] [PubMed] [Google Scholar]
- Wrigley N. G. The lattice spacing of crystalline catalase as an internal standard of length in electron microscopy. J Ultrastruct Res. 1968 Sep;24(5):454–464. doi: 10.1016/s0022-5320(68)80048-6. [DOI] [PubMed] [Google Scholar]