Abstract
Monoclonal antibodies were produced to polyhedrins from Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus (OpMNPV) and single-capsid nuclear polyhedrosis virus (OpSNPV). Although the polyhedrins are closely related, antibodies were selected which allowed differentiation between the two viruses. In an indirect enzyme-linked immunosorbent assay, purified OpMNPV and OpSNPV polyhedrins could be detected by specific monoclonal antibodies at concentrations as low as 2 and 5 ng/ml, respectively. The antibodies were also capable of identifying their homologous polyhedrin in extracts of infected insects. These antibodies would be useful for monitoring production of the viral insecticide, TM Biocontrol-1, which by license must contain only OpMNPV, and to confirm that insect mortality after aerial spraying with this insecticide is attributable to OpMNPV infection.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hohmann A. W., Faulkner P. Monoclonal antibodies to baculovirus structural proteins: determination of specificities by Western blot analysis. Virology. 1983 Mar;125(2):432–444. doi: 10.1016/0042-6822(83)90214-3. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Lane D., Koprowski H. Molecular recognition and the future of monoclonal antibodies. Nature. 1982 Mar 18;296(5854):200–202. doi: 10.1038/296200a0. [DOI] [PubMed] [Google Scholar]
- McCarthy W. H., Dobson A. J., Martyn A. L., Roberts G. Melanoma in New South Wales 1970-76: surgical therapy and survival. Aust N Z J Surg. 1982 Aug;52(4):424–430. doi: 10.1111/j.1445-2197.1982.tb06023.x. [DOI] [PubMed] [Google Scholar]
- Nowinski R. C., Lostrom M. E., Tam M. R., Stone M. R., Burnette W. N. The isolation of hybrid cell lines producing monoclonal antibodies against the p15(E) protein of ecotropic murine leukemia viruses. Virology. 1979 Feb;93(1):111–126. doi: 10.1016/0042-6822(79)90280-0. [DOI] [PubMed] [Google Scholar]
- Oakley B. R., Kirsch D. R., Morris N. R. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem. 1980 Jul 1;105(2):361–363. doi: 10.1016/0003-2697(80)90470-4. [DOI] [PubMed] [Google Scholar]
- Rohrmann G. F., Bailey T. J., Brimhall B., Becker R. R., Beaudreau G. S. Tryptic peptide analysis and NH2-terminal amino acid sequences of polyhedrins of two baculoviruses from Orgyia pseudotsugata. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4976–4980. doi: 10.1073/pnas.76.10.4976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rohrmann G. F., Beaudreau G. S. Characterization of DNA from polyhedral inclusion bodies of the nucleopolyhedrosis single-rod virus pathogenic for Orgyia pseudotsugata. Virology. 1977 Dec;83(2):474–478. doi: 10.1016/0042-6822(77)90198-2. [DOI] [PubMed] [Google Scholar]
- Rohrmann G. F. Characterization of N-polyhedrin of two baculovirus strains pathogenic for Orgyia pseudotsugata. Biochemistry. 1977 Apr 19;16(8):1631–1634. doi: 10.1021/bi00627a017. [DOI] [PubMed] [Google Scholar]
- Rohrmann G. F., McParland R. H., Martignoni M. E., Beaudreau G. S. Genetic relatedness of two nucleopolyhedrosis viruses pathogenic for Orgyia pseudotsugata. Virology. 1978 Jan;84(1):213–217. doi: 10.1016/0042-6822(78)90237-4. [DOI] [PubMed] [Google Scholar]
- Rohrmann G. F., Pearson M. N., Bailey T. J., Becker R. R., Beaudreau G. S. N-Terminal polyhedrin sequences and occluded Baculovirus evolution. J Mol Evol. 1981;17(6):329–333. doi: 10.1007/BF01734354. [DOI] [PubMed] [Google Scholar]
- Sydow G. Eine einfache und schnelle Mikromethode zur Proteinbestimmung. Acta Biol Med Ger. 1979;38(8):1141–1144. [PubMed] [Google Scholar]