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. 1984 Oct;48(4):813–817. doi: 10.1128/aem.48.4.813-817.1984

Morphological and genetic effects of benomyl on polyploid brewing yeasts: isolation of auxotrophic mutants.

C A Bilinski, A M Sills, G G Stewart
PMCID: PMC241619  PMID: 6508289

Abstract

An enrichment procedure after ethyl methanesulfonate mutagenesis and exposure to the fungicide benomyl yielded mutants auxotrophic for several amino acids from two polyploid Saccharomyces spp. Benomyl treatment was found to have a marked morphological effect on polyploid Saccharomyces cerevisiae, causing cells to adopt a characteristic doublet cell morphology in which buds are nearly as large as the parent cells. Experiments in which nuclear division was monitored in benomyl-induced doublet cells by Giemsa nuclear staining demonstrated an unusual sequence of cytological events which culminated in the formation of binucleate parental and mononucleate bud components. The frequency of formation of doublet and binucleate parent cells was found to depend on the strain employed and the benomyl concentration administered.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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