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. 1984 Oct;48(4):833–839. doi: 10.1128/aem.48.4.833-839.1984

Specificity of marine microbial surface interactions.

S H Imam, R F Bard, T R Tosteson
PMCID: PMC241623  PMID: 6508293

Abstract

The macromolecular surface components involved in intraspecific cell surface interactions of the green microalga Chlorella vulgaris and closely associated bacteria were investigated. The specific surface attachment between this alga and its associated bacteria is mediated by lectin-like macromolecules associated with the surfaces of these cells. The binding activity of these surface polymers was inhibited by specific simple sugars; this suggests the involvement of specific receptor-ligand binding sites on the interactive surfaces. Epifluorescent microscopic evaluation of bacteria-alga interactions in the presence and absence of the macromolecules that mediate these interactions showed that the glycoproteins active in these processes were specific to the microbial sources from which they were obtained. The demonstration and definition of the specificity of these interactions in mixed microbial populations may play an important role in our understanding of the dynamics of marine microbial populations in the sea.

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Selected References

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  1. Bar-Shavit Z., Ofek I., Goldman R., Mirelman D., Sharon N. Mannose residues on phagocytes as receptors for the attachment of Escherichia coli and Salmonella typhi. Biochem Biophys Res Commun. 1977 Sep 9;78(1):455–460. doi: 10.1016/0006-291x(77)91276-1. [DOI] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  3. Bubeník J., Malkovský M., Suhajová E. The latex particle adherence (LPA) assay for detection of leukocytes with adhesive surface properties. Cell Immunol. 1978 Jan;35(1):217–225. doi: 10.1016/0008-8749(78)90141-7. [DOI] [PubMed] [Google Scholar]
  4. Cone R. E., Marchalonis J. J. Cellular and humoral aspects of the influence of environmental temperature on the immune response of poikilothermic vertebrates. J Immunol. 1972 Apr;108(4):952–957. [PubMed] [Google Scholar]
  5. Costello A. H., Cisar J. O., Kolenbrander P. E., Gabriel O. Neuraminidase-dependent hamagglutination of human erythrocytes by human strains of Actinomyces viscosus and Actinomyces naeslundii. Infect Immun. 1979 Nov;26(2):563–572. doi: 10.1128/iai.26.2.563-572.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Diener E., Marchalonis J. Cellular and humoral aspects of the primary immune response of the toad, Bufo marinus. Immunology. 1970 Feb;18(2):279–293. [PMC free article] [PubMed] [Google Scholar]
  7. Friedberg E. C., Bonura T., Love J. D., McMillan S., Radany E. H., Schultz R. A. The repair of DNA damage: recent developments and new insights. J Supramol Struct Cell Biochem. 1981;16(1):91–103. doi: 10.1002/jsscb.1981.380160109. [DOI] [PubMed] [Google Scholar]
  8. Hobbie J. E., Daley R. J., Jasper S. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol. 1977 May;33(5):1225–1228. doi: 10.1128/aem.33.5.1225-1228.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nealson K. H., Platt T., Hastings J. W. Cellular control of the synthesis and activity of the bacterial luminescent system. J Bacteriol. 1970 Oct;104(1):313–322. doi: 10.1128/jb.104.1.313-322.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ofek I., Mirelman D., Sharon N. Adherence of Escherichia coli to human mucosal cells mediated by mannose receptors. Nature. 1977 Feb 17;265(5595):623–625. doi: 10.1038/265623a0. [DOI] [PubMed] [Google Scholar]
  11. Reitherman R. W., Rosen S. D., Frasier W. A., Barondes S. H. Cell surface species-specific high affinity receptors for discoidin: developmental regulation in Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3541–3545. doi: 10.1073/pnas.72.9.3541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rosen S. D., Simpson D. L., Rose J. E., Barondes S. H. Carbohydrate-binding protein from Polysphondylium pallidum implicated in intercellular adhesion. Nature. 1974 Nov 8;252(5479):128, 149-50. doi: 10.1038/252128a0. [DOI] [PubMed] [Google Scholar]
  13. Simpson D. L., Rosen S. D., Barondes S. H. Discoidin, a developmentally regulated carbohydrate-binding protein from Dictyostelium discoideum. Purification and characterization. Biochemistry. 1974 Aug 13;13(17):3487–3493. doi: 10.1021/bi00714a011. [DOI] [PubMed] [Google Scholar]
  14. Tosteson T. R., Corpe W. A. Enhancement of adhesion of the marine Chlorella vulgaris to glass. Can J Microbiol. 1975 Jul;21(7):1025–1031. doi: 10.1139/m75-152. [DOI] [PubMed] [Google Scholar]
  15. Vela G. R., Guerra C. N. On the nature of mixed cultures of Chlorella pyrenoidosa TX 71105 and various bacteria. J Gen Microbiol. 1966 Jan;42(1):123–131. doi: 10.1099/00221287-42-1-123. [DOI] [PubMed] [Google Scholar]
  16. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  17. Zimmermann R., Iturriaga R., Becker-Birck J. Simultaneous determination of the total number of aquatic bacteria and the number thereof involved in respiration. Appl Environ Microbiol. 1978 Dec;36(6):926–935. doi: 10.1128/aem.36.6.926-935.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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