Abstract
Malolactic activity from Leuconostoc oenos ML34 is tightly associated with lactic dehydrogenase. A simple and fast procedure, involving affinity chromatography on agarose-hexane-NAD (Agnad), was used to separate malolactic activity from lactic dehydrogenase and other proteins. The yield was ca. 86%, the purification was 5.2-fold, and the Km values for l-malate, NAD+ and Mn2+ were 2.8, 0.13, and 0.028 mM, respectively, at a pH optimum of 5.8.
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- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
- Brodelius P., Larsson P. O., Mosbach K. The synthesis of three AMP-analogues: N6-(6-aminohexyl)-adenosine 5'-monophosphate, N6-(6-aminohexyl)-adenosine 2',5'-bisphosphate, and N6-(6-aminohexyl)-adenosine 3',5'-bisphosphate and their application as general ligands in biospecific affinity chromatography. Eur J Biochem. 1974 Aug 15;47(1):81–89. doi: 10.1111/j.1432-1033.1974.tb03670.x. [DOI] [PubMed] [Google Scholar]
- Caspritz G., Radler F. Malolactic enzyme of Lactobacillus plantarum. Purification, properties, and distribution among bacteria. J Biol Chem. 1983 Apr 25;258(8):4907–4910. [PubMed] [Google Scholar]
- KAUFMAN S., KORKES S., DEL CAMPILLO A. Biosynthesis of dicarboxylic acids by carbon dioxide fixation. V. Further study of the "malic" enzyme of Lactobacillus arabinosus. J Biol Chem. 1951 Sep;192(1):301–312. [PubMed] [Google Scholar]
- KORKES S., DEL CAMPILLO A., OCHOA S. Biosynthesis of dicarboxylic acids by carbon dioxide fixation. IV. Isolation and properties of an adaptive "malic" enzyme from Lactobacillus arabinosus. J Biol Chem. 1950 Dec;187(2):891–905. [PubMed] [Google Scholar]
- Lonvaud-Funel A., de Saad A. M. Purification and Properties of a Malolactic Enzyme from a Strain of Leuconostoc mesenteroides Isolated from Grapes. Appl Environ Microbiol. 1982 Feb;43(2):357–361. doi: 10.1128/aem.43.2.357-361.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]