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. 1984 Nov;48(5):1020–1025. doi: 10.1128/aem.48.5.1020-1025.1984

Degradation of 4-Chlorobenzoic Acid by Arthrobacter sp

Trevor S Marks 1, Anthony R W Smith 1, Alan V Quirk 1,*
PMCID: PMC241668  PMID: 16346660

Abstract

A mixed population, enriched and established in a defined medium, from a sewage sludge inoculum was capable of complete mineralization of 4-chlorobenzoate. An organism, identified as Arthrobacter sp., was isolated from the consortium and shown to be capable of utilizing 4-chlorobenzoate as the sole carbon and energy source in pure culture. This organism (strain TM-1), dehalogenated 4-chlorobenzoate as the initial step in the degradative pathway. The product, 4-hydroxybenzoate, was further metabolized via protocatechuate. The ability of strain TM-1 to degrade 4-chlorobenzoate in liquid medium at 25°C was improved by the use of continuous culture and repeated sequential subculturing. Other chlorinated benzoates and the parent compound benzoate did not support growth of strain TM-1. An active cell extract was prepared and shown to dehalogenate 4-chloro-, 4-fluoro-, and 4-bromobenzoate. Dehalogenase activity had an optimum pH of 6.8 and an optimum temperature of 20°C and was inhibited by dissolved oxygen and stimulated by manganese (Mn2+). Strain improvement resulted in an increase in the specific activity of the cell extract from 0.09 to 0.85 nmol of 4-hydroxybenzoate per min per mg of protein and a decrease in the doubling time of the organism from 50 to 1.6 h.

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Selected References

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  1. Bird J. A., Cain R. B. cis-cis-Muconate, the product inducer of catechol 1,2-oxygenase in Pseudomonas aeruginosa. Biochem J. 1968 Sep;109(3):479–481. doi: 10.1042/bj1090479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dorn E., Hellwig M., Reineke W., Knackmuss H. J. Isolation and characterization of a 3-chlorobenzoate degrading pseudomonad. Arch Microbiol. 1974;99(1):61–70. doi: 10.1007/BF00696222. [DOI] [PubMed] [Google Scholar]
  3. Furukawa K., Tomizuka N., Kamibayashi A. Effect of chlorine substitution on the bacterial metabolism of various polychlorinated biphenyls. Appl Environ Microbiol. 1979 Aug;38(2):301–310. doi: 10.1128/aem.38.2.301-310.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goldman P., Milne G. W., Keister D. B. Carbon-halogen bond cleavage. 3. Studies on bacterial halidohrolases. J Biol Chem. 1968 Jan 25;243(2):428–434. [PubMed] [Google Scholar]
  5. Guroff G., Daly J. W., Jerina D. M., Renson J., Witkop B., Udenfriend S. Hydroxylation-induced migration: the NIH shift. Recent experiments reveal an unexpected and general result of enzymatic hydroxylation of aromatic compounds. Science. 1967 Sep 29;157(3796):1524–1530. doi: 10.1126/science.157.3796.1524. [DOI] [PubMed] [Google Scholar]
  6. KAUFMAN S. The enzymic conversion of 4-fluorophenylalanine to tyrosine. Biochim Biophys Acta. 1961 Aug 19;51:619–621. doi: 10.1016/0006-3002(61)90632-1. [DOI] [PubMed] [Google Scholar]
  7. Markus A., Klages U., Lingens F. Mikrobieller Abbau von 4-Chlorphenylessigsäure. Chemische Synthese von 3-Chlor-4-hydroxy-, 4-Chlor-3-hydroxy- und 4-Chlor-2-hydroxyphenylessigsäure. Hoppe Seylers Z Physiol Chem. 1982 Apr;363(4):431–437. [PubMed] [Google Scholar]
  8. Mires M. H., Alexander C. H. The prophylactic treatment tuberculosis. Del Med J. 1972 Jul;44(7):187–190. [PubMed] [Google Scholar]
  9. Reineke W., Knackmuss H. J. Construction of haloaromatics utilising bacteria. Nature. 1979 Feb 1;277(5695):385–386. doi: 10.1038/277385a0. [DOI] [PubMed] [Google Scholar]
  10. Ruisinger S., Klages U., Lingens F. Abbau der 4-chlorbenzoesäure durch eine Arthrobacter-Species. Arch Microbiol. 1976 Nov 2;110(23):253–256. doi: 10.1007/BF00690235. [DOI] [PubMed] [Google Scholar]
  11. Suflita J. M., Horowitz A., Shelton D. R., Tiedje J. M. Dehalogenation: a novel pathway for the anaerobic biodegradation of haloaromatic compounds. Science. 1982 Dec 10;218(4577):1115–1117. doi: 10.1126/science.218.4577.1115. [DOI] [PubMed] [Google Scholar]
  12. Weightman A. J., Weightman A. L., Slater J. H. Stereospecificity of 2-monochloropropionate dehalogenation by the two dehalogenases of Pseudomonas putida PP3: evidence for two different dehalogenation mechanisms. J Gen Microbiol. 1982 Aug;128(8):1755–1762. doi: 10.1099/00221287-128-8-1755. [DOI] [PubMed] [Google Scholar]

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