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. 1984 Dec;48(6):1171–1175. doi: 10.1128/aem.48.6.1171-1175.1984

Production of monoclonal antibodies to staphylococcal enterotoxin A.

C Edwin, S R Tatini, R S Strobel, S K Maheswaran
PMCID: PMC241704  PMID: 6517583

Abstract

Spleen cells from mice immunized with staphylococcal enterotoxin A were successfully fused with NS-1 mouse myeloma cells. Two of the four clones studied produced monoclonal antibodies to staphylococcal enterotoxin A in growth medium which showed titers of greater than 10(6) to 10(7) when tested by the indirect enzyme-linked immunosorbent assay. These monoclonal antibodies showed reactivity with enterotoxins A and E in the enzyme-linked immunosorbent assay. However, the reactivity was higher with enterotoxin A than with enterotoxin E. Nanogram quantities of crude staphylococcus enterotoxin A from Staphylococcus aureus growth were detected by the monoclonal antibodies in electroimmunoblots via autoradiography.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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