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. 1984 Dec;48(6):1189–1196. doi: 10.1128/aem.48.6.1189-1196.1984

Characterization of Limulus amoebocyte lysate-reactive material from hollow-fiber dialyzers.

F C Pearson, J Bohon, W Lee, G Bruszer, M Sagona, G Jakubowski, R Dawe, D Morrison, C Dinarello
PMCID: PMC241708  PMID: 6517586

Abstract

Hollow-fiber hemodialyzers containing cellulose-based membranes have been shown to produce positive results with the Limulus amoebocyte lysate test. This study was undertaken to determine whether endotoxin was causing the reaction. Rinses from 45 parallel-plate and hollow-fiber dialyzers from eight different manufacturers were tested before and after treatment with cellulase, using three lysates and four Limulus amoebocyte lysate methods. In addition, four in vitro cellular methods--human leukocytic pyrogen, lymphocytic activating factor, peritoneal macrophage, and arginase release--were used to evaluate endotoxin activity. The substance causing the reaction was identified by chromatographic methods. Results indicate that the Limulus amoebocyte lysate reactive material is cellulose derived and not pyrogenic.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carson L. A., Petersen N. J. LAL-reactive material associated with hemodialysis membranes. Prog Clin Biol Res. 1982;93:217–230. [PubMed] [Google Scholar]
  2. Case M. J., Novitsky T. J. Comparison of several endotoxin standards with a kinetic LAL test. Prog Clin Biol Res. 1982;93:161–171. [PubMed] [Google Scholar]
  3. Cooper J. F. Acceptance of the Limulus test as an alternative pyrogen test for radiopharmaceutical and intrathecal drugs. Prog Clin Biol Res. 1979;29:345–352. [PubMed] [Google Scholar]
  4. Doe W. F., Yang S. T., Morrison D. C., Betz S. J., Henson P. M. Macrophage stimulation by bacterial lipopolysaccharides. II. Evidence for differentiation signals delivered by lipid A and by a protein rich fraction of lipopolysaccharides. J Exp Med. 1978 Aug 1;148(2):557–568. doi: 10.1084/jem.148.2.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jorgensen J. H., Alexander G. A. Rapid detection of significant bacteriuria by use of an automated Limulus amoebocyte lysate assay. J Clin Microbiol. 1982 Sep;16(3):587–589. doi: 10.1128/jcm.16.3.587-589.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kakinuma A., Asano T., Torii H., Sugino Y. Gelation of Limulus amoebocyte lysate by an antitumor (1 leads to 3)-beta-D-glucan. Biochem Biophys Res Commun. 1981 Jul 30;101(2):434–439. doi: 10.1016/0006-291x(81)91278-x. [DOI] [PubMed] [Google Scholar]
  7. Kimura H. Measurement of endotoxin. II. Comparison of reactivities measured by radioimmunoassay and with the Limulus test. Acta Med Okayama. 1976 Aug;30(4):257–270. [PubMed] [Google Scholar]
  8. Mascoli C. C., Weary M. E. Limulus amebocyte lysate (LAL) test for detecting pyrogens in parenteral injectable products and medical devices: advantages to manufacturers and regulatory officials. J Parenter Drug Assoc. 1979 Mar-Apr;33(2):81–95. [PubMed] [Google Scholar]
  9. McCullough K. Z. The use of LAL as an alternative to the CFR rabbit pyrogen test for disodium ticarcillin. Prog Clin Biol Res. 1982;93:91–100. [PubMed] [Google Scholar]
  10. Mikami T., Nagase T., Matsumoto, Suzuki S., Suzuki M. Gelatin of Limulus amoebocyte lysate by simple polysaccharides. Microbiol Immunol. 1982;26(5):403–409. doi: 10.1111/j.1348-0421.1982.tb00190.x. [DOI] [PubMed] [Google Scholar]
  11. Nandan R., Brown D. R. An improved in vitro pyrogen test: to detect picograms of endotoxin contamination in intravenous fluids using limulus amoebocyte lysate. J Lab Clin Med. 1977 Apr;89(4):910–918. [PubMed] [Google Scholar]
  12. Pearson F. C., 3rd, Weary M. E., Bohon J., Dabbah R. Relative potency of "environmental" endotoxin as measured by the Limulus amebocyte lysate test and the USP rabbit pyrogen test. Prog Clin Biol Res. 1982;93:65–77. [PubMed] [Google Scholar]
  13. Pearson F. C., 3rd, Weary M. E., Dabbah R. A corporate approach to in-process and end-product testing with the LAL assay for endotoxin. Prog Clin Biol Res. 1982;93:231–246. [PubMed] [Google Scholar]
  14. Pearson F. C., Weary M., Bohon J. Detection of Limulus amebocyte lysate reactive material in capillary flow hemodialyzers. Prog Clin Biol Res. 1982;93:247–260. [PubMed] [Google Scholar]
  15. Petersen N. J., Carson L. A., Favero M. S. Bacterial endotoxin in new and reused hemodialyzers: a potential cause of endotoxemia. Trans Am Soc Artif Intern Organs. 1981;27:155–160. [PubMed] [Google Scholar]
  16. Prior R. B., Spagna V. A. Rapid evaluation of female patients exposed to gonorrhea by use of the Limulus lysate test. J Clin Microbiol. 1982 Sep;16(3):487–489. doi: 10.1128/jcm.16.3.487-489.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rosenwasser L. J., Dinarello C. A. Ability of human leukocytic pyrogen to enhance phytohemagglutinin induced murine thymocyte proliferation. Cell Immunol. 1981 Sep 1;63(1):134–142. doi: 10.1016/0008-8749(81)90034-4. [DOI] [PubMed] [Google Scholar]
  18. Ryan J. L., Yohe W. B., Morrison D. C. Stimulation of peritoneal cell arginase by bacterial lipopolysaccharides. Am J Pathol. 1980 May;99(2):451–462. [PMC free article] [PubMed] [Google Scholar]
  19. Siegel S. E., Nachum R. Indications for the use of the Limulus lysate assay in bacterial meningitis. Prog Clin Biol Res. 1979;29:245–253. [PubMed] [Google Scholar]
  20. Suzuki M., Mikami T., Matsumoto T., Suzuki S. Gelation of Limulus lysate by synthetic dextran derivatives. Microbiol Immunol. 1977;21(8):419–425. doi: 10.1111/j.1348-0421.1977.tb00307.x. [DOI] [PubMed] [Google Scholar]
  21. Weary M., Baker B. Utilization of the limulus amebocyte lysate test for pyrogen testing large volume parenterals, administration sets, and medical devices. Bull Parenter Drug Assoc. 1977 May-Jun;31(3):127–133. [PubMed] [Google Scholar]
  22. Wildfeuer A., Heymer B., Schleifer K. H., Haferkamp O. Investigations on the specificity of the Limulus test for the detection of endotoxin. Appl Microbiol. 1974 Nov;28(5):867–871. doi: 10.1128/am.28.5.867-871.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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