Abstract
Bacterial abundance, distribution, and heterotrophic uptake in a freshwater aquifer contaminated by treated sewage were determined from analyses of groundwater and sediment-core samples. The number of free-living (unattached) bacteria in contaminated groundwater declined steadily with increasing distance from the source of sewage infiltration, from 1.94 (+/- 0.20) X 10(6) ml-1 at 0.21 km to 0.25 (+/- 0.02) X 10(6) ml-1 at 0.97 km. Bacterial abundance in groundwater sampled at 0.31 km correlated strongly with specific conductance and increased sharply from 4.0 (+/- 0.3) X 10(4) ml-1 at a depth of 6 m to 1.58 (+/- 0.12) X 10(6) ml-1 at 14 m, then declined at 20 and 31 m to 1.29 (+/- 0.12) X 10(6) and 0.96 (+/- 0.12) X 10(6) ml-1, respectively. A majority of the bacteria in contaminated and uncontaminated zones of the aquifer were bound to the surfaces of particulates, less than 60 micron in diameter. The glucose uptake rate, assayed at in situ and 5 microM concentrations, declined steadily in contaminated groundwater sampled along a transect. A preparative wet-sieving technique for use in processing core samples for bacterial enumeration is described and evaluated.
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- Harvey R. W., Young L. Y. Enrichment and association of bacteria and particulates in salt marsh surface water. Appl Environ Microbiol. 1980 Apr;39(4):894–899. doi: 10.1128/aem.39.4.894-899.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harvey R. W., Young L. Y. Enumeration of particle-bound and unattached respiring bacteria in the salt marsh environment. Appl Environ Microbiol. 1980 Jul;40(1):156–160. doi: 10.1128/aem.40.1.156-160.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hobbie J. E., Daley R. J., Jasper S. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol. 1977 May;33(5):1225–1228. doi: 10.1128/aem.33.5.1225-1228.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ladd T. I., Ventullo R. M., Wallis P. M., Costerton J. W. Heterotrophic activity and biodegradation of labile and refractory compounds by groundwater and stream microbial populations. Appl Environ Microbiol. 1982 Aug;44(2):321–329. doi: 10.1128/aem.44.2.321-329.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]