Abstract
The Schaeffer-Fulton endospore stain was modified so that it would stain Bacillus subtilis endospores in soil smears. The modified stain differentiated among dormant spores, spores undergoing activation, and spores which had germinated but had not yet shown outgrowth. These differentiations were seen for spores in soil and for pure spore preparations in the laboratory. This stain was used to show reversible B. subtilis spore activation promoted by an Ensifer adhaerens-like indigenous bacterium in soil and by pure cultures of E. adhaerens added to spores in the laboratory. Under the specific conditions in the laboratory, spore germination did not proceed beyond the activation stage, and relatively little change occurred in the numbers of both E. adhaerens and B. subtilis. This was also true in soil, although some germination with destruction of spores and vegetative cells did occur if the soil had been nutritionally enriched by preincubation with incorporated ground alfalfa.
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- Casida L. E. Bacterial Predators of Micrococcus luteus in Soil. Appl Environ Microbiol. 1980 May;39(5):1035–1041. doi: 10.1128/aem.39.5.1035-1041.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Casida L. E., Jr Continuously variable amplitude contrast microscopy for the detection and study of microorganisms in soil. Appl Environ Microbiol. 1976 Apr;31(4):605–608. doi: 10.1128/aem.31.4.605-608.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gould G. W. Symposium on bacterial spores: IV. Germination and the problem of dormancy. J Appl Bacteriol. 1970 Mar;33(1):34–49. doi: 10.1111/j.1365-2672.1970.tb05232.x. [DOI] [PubMed] [Google Scholar]
- HERMIER J. [Germination of the Bacillus subtilis spore. I. Effect of sugars and amino acids on the initial phase of germination]. Ann Inst Pasteur (Paris) 1962 May;102:629–643. [PubMed] [Google Scholar]
- KEYNAN A., HALVORSON H. O. Calcium dipicolinic acid-induced germination of Bacillus cereus spores. J Bacteriol. 1962 Jan;83:100–105. doi: 10.1128/jb.83.1.100-105.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEVINSON H. S., HYATT M. T. The stimulation of germination and respiration of Bacillus megaterium spores by mangasese, L-alanine and heat. J Bacteriol. 1955 Oct;70(4):368–374. doi: 10.1128/jb.70.4.368-374.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Labeda D. P., Balkwill D. L., Casida L. E., Jr Soil sterilization effects on in situ indigenous microbial cells in soil. Can J Microbiol. 1975 Mar;21(3):263–269. doi: 10.1139/m75-037. [DOI] [PubMed] [Google Scholar]
- Lee W. H., Ordal Z. J. REVERSIBLE ACTIVATION FOR GERMINATION AND SUBSEQUENT CHANGES IN BACTERIAL SPORES. J Bacteriol. 1963 Jan;85(1):207–217. doi: 10.1128/jb.85.1.207-217.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lin C. C., Casida L. E. GELRITE as a Gelling Agent in Media for the Growth of Thermophilic Microorganisms. Appl Environ Microbiol. 1984 Feb;47(2):427–429. doi: 10.1128/aem.47.2.427-429.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- POWELL J. F., HUNTER J. R. Spore germination in the genus bacillus: the modification of germination requirements as a result of preheating. J Gen Microbiol. 1955 Aug;13(1):59–67. doi: 10.1099/00221287-13-1-59. [DOI] [PubMed] [Google Scholar]
- PULVERTAFT R. J. V., HAYNES J. A. Adenosine and spore germination; phase-contrast studies. J Gen Microbiol. 1951 Oct;5(4):657–663. doi: 10.1099/00221287-5-4-657. [DOI] [PubMed] [Google Scholar]