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. 1985 Jun;49(6):1488–1493. doi: 10.1128/aem.49.6.1488-1493.1985

Bacterial Biovolume and Biomass Estimations

Gunnar Bratbak 1
PMCID: PMC241752  PMID: 16346817

Abstract

The biomass of bacterial populations in aquatic ecosystems is often estimated by measuring bacterial biovolume and converting this into biomass in terms of carbon. A reliable conversion factor relating the measured bacterial biovolume to bacterial carbon content is essential for this approach. Based on direct measurements of bacterial cell carbon content, cell number, and biovolume, I have derived an average conversion factor of 5.6 × 10−13 g of C μm−3. This conversion factor is 3.4 to 6.6 times higher than most theoretically derived factors currently in use. Both bacterial biomass and bacterial production in aquatic ecosystems may thus have been seriously underestimated.

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Selected References

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  1. Bakken L. R., Olsen R. A. Buoyant densities and dry-matter contents of microorganisms: conversion of a measured biovolume into biomass. Appl Environ Microbiol. 1983 Apr;45(4):1188–1195. doi: 10.1128/aem.45.4.1188-1195.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bell R. T., Ahlgren G. M., Ahlgren I. Estimating Bacterioplankton Production by Measuring [H]thymidine Incorporation in a Eutrophic Swedish Lake. Appl Environ Microbiol. 1983 Jun;45(6):1709–1721. doi: 10.1128/aem.45.6.1709-1721.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bowden W. B. Comparison of two direct-count techniques for enumerating aquatic bacteria. Appl Environ Microbiol. 1977 May;33(5):1229–1232. doi: 10.1128/aem.33.5.1229-1232.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bratbak G., Dundas I. Bacterial dry matter content and biomass estimations. Appl Environ Microbiol. 1984 Oct;48(4):755–757. doi: 10.1128/aem.48.4.755-757.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fuhrman J. A., Azam F. Bacterioplankton secondary production estimates for coastal waters of british columbia, antarctica, and california. Appl Environ Microbiol. 1980 Jun;39(6):1085–1095. doi: 10.1128/aem.39.6.1085-1095.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hagström A., Larsson U., Hörstedt P., Normark S. Frequency of dividing cells, a new approach to the determination of bacterial growth rates in aquatic environments. Appl Environ Microbiol. 1979 May;37(5):805–812. doi: 10.1128/aem.37.5.805-812.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hobbie J. E., Daley R. J., Jasper S. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol. 1977 May;33(5):1225–1228. doi: 10.1128/aem.33.5.1225-1228.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Montesinos E., Esteve I., Guerrero R. Comparison between direct methods for determination of microbial cell volume: electron microscopy and electronic particle sizing. Appl Environ Microbiol. 1983 May;45(5):1651–1658. doi: 10.1128/aem.45.5.1651-1658.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Muller L. L., Jacks T. J. Rapid chemical dehydration of samples for electron microscopic examinations. J Histochem Cytochem. 1975 Feb;23(2):107–110. doi: 10.1177/23.2.1117127. [DOI] [PubMed] [Google Scholar]
  10. Pedrós-Alió C., Brock T. D. Assessing biomass and production of bacteria in eutrophic lake mendota, wisconsin. Appl Environ Microbiol. 1982 Jul;44(1):203–218. doi: 10.1128/aem.44.1.203-218.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tempest D. W., Dicks J. W., Ellwood D. C. Influence of growth condition on the concentration of potassium in Bacillus subtilis var. niger and its possible relationship to cellular ribonucleic acid, teichoic acid and teichuronic acid. Biochem J. 1968 Jan;106(1):237–243. doi: 10.1042/bj1060237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Trueba F. J., Woldringh C. L. Changes in cell diameter during the division cycle of Escherichia coli. J Bacteriol. 1980 Jun;142(3):869–878. doi: 10.1128/jb.142.3.869-878.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Watson S. W., Novitsky T. J., Quinby H. L., Valois F. W. Determination of bacterial number and biomass in the marine environment. Appl Environ Microbiol. 1977 Apr;33(4):940–946. doi: 10.1128/aem.33.4.940-946.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Woldringh C. L., de Jong M. A., van den Berg W., Koppes L. Morphological analysis of the division cycle of two Escherichia coli substrains during slow growth. J Bacteriol. 1977 Jul;131(1):270–279. doi: 10.1128/jb.131.1.270-279.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. van Veen J. A., Paul E. A. Conversion of biovolume measurements of soil organisms, grown under various moisture tensions, to biomass and their nutrient content. Appl Environ Microbiol. 1979 Apr;37(4):686–692. doi: 10.1128/aem.37.4.686-692.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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