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. 1985 Jun;49(6):1534–1537. doi: 10.1128/aem.49.6.1534-1537.1985

Growth of Legionella pneumophila in continuous culture.

J D Berg, J C Hoff, P V Roberts, A Matin
PMCID: PMC241762  PMID: 4015091

Abstract

A method was developed to grow Legionella pneumophila in continuous culture. A chemostat was used to simulate nutrient-limited, submaximal growth in the natural environmental and to provide a precisely controlled growth regimen. Cultures grew under forced aeration under conditions yielding up to 38% saturation of dissolved oxygen; supplemental CO2 (5%) at the same gas flow rates as ambient air had no effect on culture growth. Pleomorphism was observed during growth under all conditions. Pigment was produced only at D less than 0.03 h-1. Catalase was produced at higher growth rates but not at higher temperatures. The pathogenicity was unaffected by altering either the growth rate or the growth temperature.

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Selected References

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