Abstract
By cloning the β-galactosidase gene of Bacillus stearothermophilus IAM11001 (ATCC 8005) into Bacillus subtilis, enzyme production was enhanced 50 times. β-Galactosidase could be purified to 80% homogeneity by incubating the cell extract of B. subtilis at 70°C for 15 min, followed by centrifugation to remove the denatured proteins. Because of its heat stability and ease of production, β-galactosidase is suitable for application in industrial processes.
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- CRAVEN G. R., STEERS E., Jr, ANFINSEN C. B. PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12. J Biol Chem. 1965 Jun;240:2468–2477. [PubMed] [Google Scholar]
- Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goodman R. E., Pederson D. M. beta-Galactosidase from Bacillus stearothermophilus. Can J Microbiol. 1976 Jun;22(6):817–825. doi: 10.1139/m76-118. [DOI] [PubMed] [Google Scholar]
- Griffiths M. W., Muir D. D. Properties of a thermostable beta-galactosidase from a thermophilic Bacillus: comparison of the enzyme activity of whole cells, purified enzyme and immobilised whole cells. J Sci Food Agric. 1978 Sep;29(9):753–761. doi: 10.1002/jsfa.2740290904. [DOI] [PubMed] [Google Scholar]
- Gryczan T. J., Contente S., Dubnau D. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J Bacteriol. 1978 Apr;134(1):318–329. doi: 10.1128/jb.134.1.318-329.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hirata H., Negoro S., Okada H. Molecular basis of isozyme formation of beta-galactosidases in Bacillus stearothermophilus: isolation of two beta-galactosidase genes, bgaA and bgaB. J Bacteriol. 1984 Oct;160(1):9–14. doi: 10.1128/jb.160.1.9-14.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Tanaka T., Kawano N., Oshima T. Cloning of 3-isopropylmalate dehydrogenase gene of an extreme thermophile and partial purification of the gene product. J Biochem. 1981 Feb;89(2):677–682. doi: 10.1093/oxfordjournals.jbchem.a133245. [DOI] [PubMed] [Google Scholar]