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. 1982 Jan;43(1):70–74. doi: 10.1128/aem.43.1.70-74.1982

Growth of Eubacterium limosum with Carbon Monoxide as the Energy Source

B R Sharak Genthner 1, M P Bryant 1
PMCID: PMC241782  PMID: 16345931

Abstract

Eubacterium limosum grew with CO as the sole source of energy and formed acetate and CO2 as the major products. The generation time on CO was 7 h. Uninhibited growth occurred in cultures containing 50% CO or less, but growth occurred at all concentrations tested (i.e., up to 75% CO). The pH optimum for growth was 7.0 to 7.2, whereas growth was poor at a pH below 6.7. CO2 stimulated growth on CO. CO was preferentially utilized when both CO and H2 were present.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andreesen J. R., Gottschalk G., Schlegel H. G. Clostridium formicoaceticum nov. spec. isolation, description and distinction from C. aceticum and C. thermoaceticum. Arch Mikrobiol. 1970;72(2):154–174. doi: 10.1007/BF00409521. [DOI] [PubMed] [Google Scholar]
  2. Daniels L., Fuchs G., Thauer R. K., Zeikus J. G. Carbon monoxide oxidation by methanogenic bacteria. J Bacteriol. 1977 Oct;132(1):118–126. doi: 10.1128/jb.132.1.118-126.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Diekert G. B., Thauer R. K. Carbon monoxide oxidation by Clostridium thermoaceticum and Clostridium formicoaceticum. J Bacteriol. 1978 Nov;136(2):597–606. doi: 10.1128/jb.136.2.597-606.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Drake H. L., Hu S. I., Wood H. G. Purification of carbon monoxide dehydrogenase, a nickel enzyme from Clostridium thermocaceticum. J Biol Chem. 1980 Aug 10;255(15):7174–7180. [PubMed] [Google Scholar]
  5. Fontaine F. E., Peterson W. H., McCoy E., Johnson M. J., Ritter G. J. A New Type of Glucose Fermentation by Clostridium thermoaceticum. J Bacteriol. 1942 Jun;43(6):701–715. doi: 10.1128/jb.43.6.701-715.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GRAY C. T., GEST H. BIOLOGICAL FORMATION OF MOLECULAR HYDROGEN. Science. 1965 Apr 9;148(3667):186–192. doi: 10.1126/science.148.3667.186. [DOI] [PubMed] [Google Scholar]
  7. Genthner B. R., Davis C. L., Bryant M. P. Features of rumen and sewage sludge strains of Eubacterium limosum, a methanol- and H2-CO2-utilizing species. Appl Environ Microbiol. 1981 Jul;42(1):12–19. doi: 10.1128/aem.42.1.12-19.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ghambeer R. K., Wood H. G., Schulman M., Ljungdahl L. Total synthesis of acetate from CO2. 3. Inhibition by alkylhalides of the synthesis from CO2, methyltetrahydrofolate, and methyl-B12 by Clostridium thermoaceticum. Arch Biochem Biophys. 1971 Apr;143(2):471–484. doi: 10.1016/0003-9861(71)90232-3. [DOI] [PubMed] [Google Scholar]
  9. PUREC L., KRASNA A. I., RITTENBERG D. The inhibition of hydrogenase by carbon monoxide and the reversal of this inhibition by light. Biochemistry. 1962 Mar;1:270–275. doi: 10.1021/bi00908a013. [DOI] [PubMed] [Google Scholar]
  10. Postgate J. Carbon monoxide as a basis for primitive life on other planets: a comment. Nature. 1970 Jun 6;226(5249):978–978. doi: 10.1038/226978a0. [DOI] [PubMed] [Google Scholar]
  11. Tanner R. S., Wolfe R. S., Ljungdahl L. G. Tetrahydrofolate enzyme levels in Acetobacterium woodii and their implication in the synthesis of acetate from CO2. J Bacteriol. 1978 May;134(2):668–670. doi: 10.1128/jb.134.2.668-670.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Thauer R. K., Fuchs G., Käufer B., Schnitker U. Carbon-monoxide oxidation in cell-free extracts of Clostridium pasteurianum. Eur J Biochem. 1974 Jun 15;45(2):343–349. doi: 10.1111/j.1432-1033.1974.tb03559.x. [DOI] [PubMed] [Google Scholar]
  13. Thauer R. K., Käufer B., Zähringer M., Jungermann K. The reaction of the iron-sulfur protein hydrogenase with carbon monoxide. Eur J Biochem. 1974 Mar 1;42(2):447–452. doi: 10.1111/j.1432-1033.1974.tb03358.x. [DOI] [PubMed] [Google Scholar]
  14. Uffen R. L. Anaerobic growth of a Rhodopseudomonas species in the dark with carbon monoxide as sole carbon and energy substrate. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3298–3302. doi: 10.1073/pnas.73.9.3298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. YAGI T. Enzymic oxidation of carbon monoxide. Biochim Biophys Acta. 1958 Oct;30(1):194–195. doi: 10.1016/0006-3002(58)90263-4. [DOI] [PubMed] [Google Scholar]

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