Abstract
The successful use of transposon mutagenesis in a slow-growing Rhizobium permitted the isolation of auxotrophic and symbiotically defective mutants. In these mutants, it was shown that different single-copy Tn5 insertions had occurred. Mutant phenotypes indicated that the control of nitrogenase activity in vitro and in planta is different.
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- Coventry D. R., Trinick M. J., Appleby C. A. A search for a leghaemoglobin-like compound in root nodules of Trema cannabina Lour. Biochim Biophys Acta. 1976 Jan 20;420(1):105–111. doi: 10.1016/0005-2795(76)90349-4. [DOI] [PubMed] [Google Scholar]
- Pühler A., Burkardt H. J., Klipp W. Cloning of the entire region for nitrogen fixation from Klebsiella pneumoniae on a multicopy plasmid vehicle in Escherichia coli. Mol Gen Genet. 1979 Oct 2;176(1):17–24. doi: 10.1007/BF00334290. [DOI] [PubMed] [Google Scholar]
- Trinick M. J. Structure of nitrogen-fixing nodules formed by Rhizobium on roots of Parasponia andersonii Planch. Can J Microbiol. 1979 May;25(5):565–578. doi: 10.1139/m79-082. [DOI] [PubMed] [Google Scholar]