Abstract
In a previous study, transformation demonstrated that a gene governing enterotoxin A production (entA+) in Staphylococcus aureus strain S-6 was located on the chromosome between the purB110 and ilv-129 markers; in contrast, the entA+ gene of strain FRI-196E was shown not to be located in the same position. In the current study, 54 enterotoxin A-producing strains of S. aureus were examined to locate the entA+ gene. Conventional transformation procedures and a series of multiply marked derivatives of NCTC 8325 were used as recipients for chromosomal mapping. Of the 54 strains tested, 23 were found to contain the entA+ gene at the original locus between the purB110 and ilv-129 markers. Twenty-seven strains could not be analyzed either because their DNA was genetically ineffective in transforming strain 8325 (23 strains), or Pur+ Ilv+ transformants could not be recovered (four strains). Four other strains contained an entA+ gene that could not be located in any of the chromosomal linkage groups. A new insertion site for Tn551 was located within the hla+ gene involved in alpha-toxin production. It eliminated alpha-toxin production and was used to separate the entA+ gene from the hla+ marker in the purB110-ilv-129 region. This segment of the chromosome is shown to consist of the purB110, entA+, hla+, and ilv-129 markers in that order.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bergdoll M. S., Crass B. A., Reiser R. F., Robbins R. N., Davis J. P. A new staphylococcal enterotoxin, enterotoxin F, associated with toxic-shock-syndrome Staphylococcus aureus isolates. Lancet. 1981 May 9;1(8228):1017–1021. doi: 10.1016/s0140-6736(81)92186-3. [DOI] [PubMed] [Google Scholar]
- Brown D. R., Pattee P. A. Identification of a chromosomal determinant of alpha-toxin production in Staphylococcus aureus. Infect Immun. 1980 Oct;30(1):36–42. doi: 10.1128/iai.30.1.36-42.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dyer D. W., Iandolo J. J. Plasmid-chromosomal transition of genes important in staphylococcal enterotoxin B expression. Infect Immun. 1981 Aug;33(2):450–458. doi: 10.1128/iai.33.2.450-458.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pattee P. A. Distribution of Tn551 insertion sites responsible for auxotrophy on the Staphylococcus aureus chromosome. J Bacteriol. 1981 Jan;145(1):479–488. doi: 10.1128/jb.145.1.479-488.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pattee P. A. Genetic linkage of chromosomal tetracycline resistance and pigmentation to a purine auxotrophic marker and the isoleucine-valine-leucine structural genes in Staphylococcus aureus. J Bacteriol. 1976 Sep;127(3):1167–1172. doi: 10.1128/jb.127.3.1167-1172.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pattee P. A., Glatz B. A. Identification of a chromosomal determinant of enterotoxin A production in Staphylococcus aureus. Appl Environ Microbiol. 1980 Jan;39(1):186–193. doi: 10.1128/aem.39.1.186-193.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pattee P. A., Neveln D. S. Transformation analysis of three linkage groups in Staphylococcus aureus. J Bacteriol. 1975 Oct;124(1):201–211. doi: 10.1128/jb.124.1.201-211.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pattee P. A., Thompson N. E., Haubrich D., Novick R. P. Chromosomal map locations of integrated plasmids and related elements in Staphylococcus aureus. Plasmid. 1977 Nov;1(1):38–51. doi: 10.1016/0147-619x(77)90007-5. [DOI] [PubMed] [Google Scholar]
- Shafer W. M., Iandolo J. J. Staphylococcal enterotoxin A: a chromosomal gene product. Appl Environ Microbiol. 1978 Aug;36(2):389–391. doi: 10.1128/aem.36.2.389-391.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thompson N. E., Pattee P. A. Transformation in Staphylococcus aureus: role of bacteriophage and incidence of competence among strains. J Bacteriol. 1977 Feb;129(2):778–788. doi: 10.1128/jb.129.2.778-788.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]