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. 1982 Feb;43(2):462–468. doi: 10.1128/aem.43.2.462-468.1982

Methanogenesis in Big Soda Lake, Nevada: an Alkaline, Moderately Hypersaline Desert Lake

Ronald S Oremland 1, Lorraine Marsh 1, David J DesMarais 1
PMCID: PMC241849  PMID: 16345952

Abstract

Incubated sediment slurries from Big Soda Lake, Nevada, produced significant levels of CH4, and production was inhibited by 2-bromoethanesulfonic acid and by autoclaving. Methane production was stimulated by methanol, trimethylamine, and, to a lesser extent, methionine. Surprisingly, hydrogen, acetate, and formate amendments provided only slight or no stimulation of methanogenesis. Methane production by sediment slurries had a pH optimum of 9.7. A methanol-grown enrichment culture containing a small, epifluorescent coccus as the predominant organism was recovered from sediments. The enrichment grew best when FeS or autoclaved sediment particles were included in the media, had a pH optimum of 9.7, and produced 14CH4 from 14CH3OH. The methane formed by methanolgrown enrichment cultures was depleted in 13C by 72 to 77‰ relative to the methanol.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abram J. W., Nedwell D. B. Inhibition of methanogenesis by sulphate reducing bacteria competing for transferred hydrogen. Arch Microbiol. 1978 Apr 27;117(1):89–92. doi: 10.1007/BF00689356. [DOI] [PubMed] [Google Scholar]
  2. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. Methanogens: reevaluation of a unique biological group. Microbiol Rev. 1979 Jun;43(2):260–296. doi: 10.1128/mr.43.2.260-296.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brock T. D., Od'ea K. Amorphous ferrous sulfide as a reducing agent for culture of anaerobes. Appl Environ Microbiol. 1977 Feb;33(2):254–256. doi: 10.1128/aem.33.2.254-256.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cappenberg T. E. Interrelations between sulfate-reducing and methane-producing bacteria in bottom deposits of a fresh-water lake. II. Inhibition experiments. Antonie Van Leeuwenhoek. 1974;40(2):297–306. doi: 10.1007/BF00394388. [DOI] [PubMed] [Google Scholar]
  5. Cappenberg T. E., Prins R. A. Interrelations between sulfate-reducing and methane-producing bacteria in bottom deposits of a fresh-water lake. 3. Experiments with 14C-labeled substrates. Antonie Van Leeuwenhoek. 1974;40(3):457–469. doi: 10.1007/BF00399358. [DOI] [PubMed] [Google Scholar]
  6. Edwards T., McBride B. C. New method for the isolation and identification of methanogenic bacteria. Appl Microbiol. 1975 Apr;29(4):540–545. doi: 10.1128/am.29.4.540-545.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Godsy E. M. Isolation of Methanobacterium bryantii from a Deep Aquifer by Using a Novel Broth-Antibiotic Disk Method. Appl Environ Microbiol. 1980 May;39(5):1074–1075. doi: 10.1128/aem.39.5.1074-1075.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gunsalus R. P., Romesser J. A., Wolfe R. S. Preparation of coenzyme M analogues and their activity in the methyl coenzyme M reductase system of Methanobacterium thermoautotrophicum. Biochemistry. 1978 Jun 13;17(12):2374–2377. doi: 10.1021/bi00605a019. [DOI] [PubMed] [Google Scholar]
  9. Hayes A. W., King R. E., Unger P. D., Phillips T. D., Hatkin J., Bowen J. H. Aflatoxicosis in swine. J Am Vet Med Assoc. 1978 Jun 1;172(11):1295–1297. [PubMed] [Google Scholar]
  10. Hungate R. E. Hydrogen as an intermediate in the rumen fermentation. Arch Mikrobiol. 1967;59(1):158–164. doi: 10.1007/BF00406327. [DOI] [PubMed] [Google Scholar]
  11. Martens C. S., Berner R. A. Methane production in the interstitial waters of sulfate-depleted marine sediments. Science. 1974 Sep 27;185(4157):1167–1169. doi: 10.1126/science.185.4157.1167. [DOI] [PubMed] [Google Scholar]
  12. Oremland R. S. Microbial formation of ethane in anoxic estuarine sediments. Appl Environ Microbiol. 1981 Jul;42(1):122–129. doi: 10.1128/aem.42.1.122-129.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. ROSENFELD W. D., SILVERMAN S. R. Carbon isotope fractionation in bacterial production of methane. Science. 1959 Dec 11;130(3389):1658–1659. doi: 10.1126/science.130.3389.1658-a. [DOI] [PubMed] [Google Scholar]
  14. Shokes R. F., Trabant P. K., Presley B. J., Reid D. F. Anoxic, hypersaline basin in the northern gulf of Mexico. Science. 1977 Jun 24;196(4297):1443–1446. doi: 10.1126/science.196.4297.1443. [DOI] [PubMed] [Google Scholar]
  15. Smith P. H., Mah R. A. Kinetics of acetate metabolism during sludge digestion. Appl Microbiol. 1966 May;14(3):368–371. doi: 10.1128/am.14.3.368-371.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Souza K. A., Deal P. H., Mack H. M., Turnbill C. E. Growth and reproduction of microorganisms under extremely alkaline conditions. Appl Microbiol. 1974 Dec;28(6):1066–1068. doi: 10.1128/am.28.6.1066-1068.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ward D. M. Thermophilic methanogenesis in a hot-spring algal-bacterial mat (71 to 30 degrees C). Appl Environ Microbiol. 1978 Jun;35(6):1019–1026. doi: 10.1128/aem.35.6.1019-1026.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Winfrey M. R., Nelson D. R., Klevickis S. C., Zeikus J. G. Association of hydrogen metabolism with methanogenesis in Lake Mendota sediments. Appl Environ Microbiol. 1977 Feb;33(2):312–318. doi: 10.1128/aem.33.2.312-318.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Winfrey M. R., Zeikus J. G. Effect of sulfate on carbon and electron flow during microbial methanogenesis in freshwater sediments. Appl Environ Microbiol. 1977 Feb;33(2):275–281. doi: 10.1128/aem.33.2.275-281.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Woese C. R., Magrum L. J., Fox G. E. Archaebacteria. J Mol Evol. 1978 Aug 2;11(3):245–251. doi: 10.1007/BF01734485. [DOI] [PubMed] [Google Scholar]
  21. Zinder S. H., Brock T. D. Methane, carbon dioxide, and hydrogen sulfide production from the terminal methiol group of methionine by anaerobic lake sediments. Appl Environ Microbiol. 1978 Feb;35(2):344–352. doi: 10.1128/aem.35.2.344-352.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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