Abstract
The bioconversion of waste paper to single-cell protein at pH <1 by Scytalidium acidophilum is described. Waste paper pretreated with 72% H2SO4 at 4°C was diluted with water to a pH of <0.1 and hydrolyzed. This yielded an adequate sugar-containing substrate for the growth of the fungus. A total of 97% of the sugars (glucose, galactose, mannose, xylose, arabinose) in the hydrolysates were converted to cell biomass. Microbial contamination was not observed. Based on the sugars consumed, S. acidophilum produced higher yields in shake cultures than many other Fungi Imperfecti. In aerated cultures, productivity increased, and yields of 43 to 46% containing 44 to 47% crude protein were obtained. This compares favorably with Candida utilis, a yeast used commercially to produce single-cell protein. The chemical constituents and the essential amino acids of the fungal cells were similar to those of other fungi. The nucleic acid content was characteristic of microbes containing low levels of nucleic acid. The advantages of using S. acidophilum for single-cell protein production are discussed.
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- BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gould W. D., Fujikawa J. I., Cook F. D. A soil fungus tolerant to extreme acidity and high salt concentrations. Can J Microbiol. 1974 Jul;20(7):1023–1027. doi: 10.1139/m74-158. [DOI] [PubMed] [Google Scholar]
- Han Y. W. Microbial fermentation of rice straw: nutritive composition and in vitro digestibility of the fermentation products. Appl Microbiol. 1975 Apr;29(4):510–514. doi: 10.1128/am.29.4.510-514.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kharatyan S. G. Microbes as food for humans. Annu Rev Microbiol. 1978;32:301–327. doi: 10.1146/annurev.mi.32.100178.001505. [DOI] [PubMed] [Google Scholar]
- MCDANIEL L. E., BAILEY E. G., ZIMMERLI A. EFFECT OF OXYGEN SUPPLY RATES ON GROWTH OF ESCHERICHIA COLI. Appl Microbiol. 1965 Jan;13:109–114. doi: 10.1128/am.13.1.109-114.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sigler L., Carmichael J. W. A new acidophilic Scytalidium. Can J Microbiol. 1974 Feb;20(2):267–268. doi: 10.1139/m74-043. [DOI] [PubMed] [Google Scholar]
- Starkey R. L., Waksman S. A. Fungi Tolerant to Extreme Acidity and High Concentrations of Copper Sulfate. J Bacteriol. 1943 May;45(5):509–519. doi: 10.1128/jb.45.5.509-519.1943. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Updegraff D. M. Semimicro determination of cellulose in biological materials. Anal Biochem. 1969 Dec;32(3):420–424. doi: 10.1016/s0003-2697(69)80009-6. [DOI] [PubMed] [Google Scholar]
- Updegraff D. M. Utilization of cellulose from waste paper by Myrothecium verrucaria. Biotechnol Bioeng. 1971 Jan;13(1):77–97. doi: 10.1002/bit.260130106. [DOI] [PubMed] [Google Scholar]